Journal:

Article Title: Differential Tissue-Specific Regulation of Antiviral CD8 + T-Cell Immune Responses during Chronic Viral Infection

doi: 10.1128/JVI.78.7.3578-3600.2004

Figure Lengend Snippet: Virus-specific CD8+ T cells in nonlymphoid tissues experience functional exhaustion with a hierarchical loss of IL-2, TNF-α, and IFN-γ during chronic infections. C57BL/6 mice were infected with 102 (A) or with 2 × 106 (B) PFU of LCMV-Docile, and lymphocytes were isolated from the spleen and liver at the indicated times. The total numbers of virus- or GP133-41 or NP396-404 epitope-specific CD8+ T cells producing IFN-γ (•), TNF-α (▴), or IL-2 (▾) after short-term culturing with virus-infected DC2.4 cells or peptide, respectively, are shown. For a comparison, the numbers of total (sum of GP133-41 and NP396-404) (left panels), GP133-41 (middle panels), or NP396-404 (right panels) peptide-specific CD8+ T cells were determined by staining with H-2Db tetramers (▪). Data shown are means ± SEM of log10 virus-specific T cells per tissue for 5 to 10 mice.

Article Snippet: All stimulation reactions were performed for 6 h at 37°C in the presence of 10 U of murine interleukin-2 (IL-2)/well and 1 μg of brefeldin A (BD-Pharmingen)/well.

Techniques: Functional Assay, Infection, Isolation, Staining

Journal:

Article Title: Differential Tissue-Specific Regulation of Antiviral CD8 + T-Cell Immune Responses during Chronic Viral Infection

doi: 10.1128/JVI.78.7.3578-3600.2004

Figure Lengend Snippet: Phenotypic analysis of virus-specific CD8+ T cells from the spleen and liver during acute versus persistent infections. Lymphocytes isolated from the spleens (A) or livers (B) of C57BL/6 mice infected with 102 PFU (red histograms) or 2 × 106 PFU (open, thickly lined histograms) of LCMV-Docile at the indicated times after infection were triple stained with anti-CD8α, an antibody specific for CD11a (LFA-1), CD25 (IL-2-Rα), CD122 (IL-2-Rβ), CD44, 1B11 (CD43), CD69, CD62L, or Ly-6C, and the Db/GP133-41 tetramer. As a control, cells from uninfected mice were double stained for CD8α and the activation markers listed above (green histograms). Histograms are gated on cells that were positive for CD8α and Db/GP133-41 tetramer. These results are representative of three separate experiments.

Article Snippet: All stimulation reactions were performed for 6 h at 37°C in the presence of 10 U of murine interleukin-2 (IL-2)/well and 1 μg of brefeldin A (BD-Pharmingen)/well.

Techniques: Isolation, Infection, Staining, Activation Assay