Journal: Emerging Infectious Diseases

Article Title: Molecular Evidence of Pneumocystis Transmission in Pediatric Transplant Unit

doi: 10.3201/eid1102.040820

Figure Lengend Snippet: Pneumocystis jirovecii genotyping by PCR-SSCP of 4 genomic regions*

Article Snippet: Proceeding on the assumption that PCP in the 4 children resulted from patient-to-patient transmission, we investigated the genotypes of P. jirovecii with the multitarget single-strand conformation polymorphism (SSCP) method.

Techniques:

Journal: ACS Chemical Neuroscience

Article Title: Scaffold Hybridization Strategy Leads to the Discovery of Dopamine D 3 Receptor-Selective or Multitarget Bitopic Ligands Potentially Useful for Central Nervous System Disorders

doi: 10.1021/acschemneuro.1c00368

Figure Lengend Snippet: Affinity Values ( K i ) a of 1–11 at Human D 2 -like Receptor Subtypes and of 1–3 , 6 , 9 , Clozapine, and Olanzapine at Human D 1 R, 5-HT 1A R, 5-HT 2A R, and 5-HT 2C R

Article Snippet: Compounds 6 and 9 also display negligible D 1 R affinity but significantly lower K i values at the serotoninergic 5-HT 1A R, 5-HT 2A R, and 5-HT 2C R subtypes and, therefore, are characterized by a more balanced 5-HT/D 2 -like multitarget profile.

Techniques: Clone Assay

Journal: ACS Chemical Neuroscience

Article Title: Scaffold Hybridization Strategy Leads to the Discovery of Dopamine D 3 Receptor-Selective or Multitarget Bitopic Ligands Potentially Useful for Central Nervous System Disorders

doi: 10.1021/acschemneuro.1c00368

Figure Lengend Snippet: Potency (EC 50 or IC 50 ) a and Efficacy (% Stimulation or % Inhibition) b Values of 3 , 6 , and 9 at D 1 R-D 4 R, 5-HT 1A R, 5-HT 2A R, and 5-HT 2C R

Article Snippet: Compounds 6 and 9 also display negligible D 1 R affinity but significantly lower K i values at the serotoninergic 5-HT 1A R, 5-HT 2A R, and 5-HT 2C R subtypes and, therefore, are characterized by a more balanced 5-HT/D 2 -like multitarget profile.

Techniques: Inhibition, Functional Assay, cAMP Assay, Binding Assay

Journal: Scientific Reports

Article Title: THC-induced behavioral stereotypy in zebrafish as a model of psychosis-like behavior

doi: 10.1038/s41598-021-95016-4

Figure Lengend Snippet: Co-administration of Tetrahydrocannabinol (THC) and GABA A receptor antagonist pentylenetetrazol (PTZ) affects the THC-induced behavioral stereotypy in adult zebrafish. ( A ) At 1.5 mM PTZ with 1 μM THC the THC-induced Repetition Index (RI), calculated as a mean ± SEM, was not statistically different from the controls (EtOH) (ns p = 0.1578). ( B ) 1.5 mM PTZ and 1 mM PTZ with 1 μM THC caused significant increases in swimming velocity compared to controls (EtOH) (* p < 0.05). Controls EtOH (0.0015%) (n = 19), 1 μM THC (n = 23), 1.5 mM PTZ (n = 12), 0.2 mM PTZ + 1 μM THC (n = 6), 1 mM PTZ + 1 μM THC (n = 5), 1.5 mM PTZ + 1 μM THC (n = 12) and 2 mM PTZ + 1 μM THC (n = 6). Mean RI values of 0.2 mM PTZ (n = 6) and 2 mM PTZ (n = 6), were similar to 1.5 mM PTZ. Values without a letter in common are statistically different to the control condition ( p < 0.05, Kruskal–Wallis and Dunn's multiple comparisons test).

Article Snippet: Despite the multitude of CB 1 R pathways for THC to exert its effects on glutamate, GABA and downstream DA signaling, reports of THC as a multitarget ligand may better explain the non-CB 1 R mediated THC behavioral stereotypy .

Techniques: Control

Journal: Scientific Reports

Article Title: THC-induced behavioral stereotypy in zebrafish as a model of psychosis-like behavior

doi: 10.1038/s41598-021-95016-4

Figure Lengend Snippet: Potential mechanisms underlying the associations between Cannabis consumption and risks of psychosis/schizophrenia-like symptoms, with the current study’s pharmacological approaches. Δ9-tetrahydrocannabinol (THC) reduces N-methyl-D-aspartate (NMDA) release via NMDA receptor antagonism. THC also inhibits γ‐aminobutyric acid (GABA) release from interneurons in the prefrontal cortex, potentiating dopamine release. Both NMDA receptor hypoactivity and heightened dopaminergic signaling are hallmarks of schizophrenia. Pentylenetetrazol (PTZ).

Article Snippet: Despite the multitude of CB 1 R pathways for THC to exert its effects on glutamate, GABA and downstream DA signaling, reports of THC as a multitarget ligand may better explain the non-CB 1 R mediated THC behavioral stereotypy .

Techniques: Cannabis