Journal: International Journal of Molecular Sciences

Article Title: Colonic Mucosal Immune Activation in Mice with Ovalbumin-Induced Allergic Airway Disease: Association between Allergic Airway Disease and Irritable Bowel Syndrome

doi: 10.3390/ijms23010181

Figure Lengend Snippet: The results of cytokine ELISA assay. T-helper 1 (Th1) (IL-1β, interferon [IFN]-γ, and tumor necrosis factor [TNF]-α), Th2 (IL-4, IL-5 and IL-10), and proinflammatory cytokine (IL-6) levels were measured using ELISA with a multiplex protein assay kit. * p < 0.05, ** p = 0.064.

Article Snippet: A multiplex protein assay kit (Bio-Rad, Hercules, CA, USA) was used to measure the mucosal levels of Th1 (IFN-γ, IL-1β and TNF-α), Th2 (IL-4, IL-5 and IL-10), and proinflammatory cytokines (IL-6).

Techniques: Enzyme-linked Immunosorbent Assay, Multiplex Assay

Journal: Cell

Article Title: The Parkinson’s disease protein alpha-synuclein is a modulator of Processing-bodies and mRNA stability

doi: 10.1016/j.cell.2022.05.008

Figure Lengend Snippet: Key resources table

Article Snippet: BCA Protein Assay Kit , Pierce , 23225.

Techniques: Western Blot, Virus, Recombinant, Protease Inhibitor, Lysis, Magnetic Beads, Membrane, Transfection, Expressing, Bicinchoninic Acid Protein Assay, Silver Staining, In Situ, Sample Prep, Luciferase, Reporter Assay, Multiplex sample analysis, Biomarker Discovery, Marker, Generated, Software, Mass Spectrometry, Imaging

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Identification of Tengfu Jiangya Tablet Target Biomarkers with Quantitative Proteomic Technique

doi: 10.1155/2017/7594805

Figure Lengend Snippet: Bioinformatics analysis of differentially expressed proteins. GO analysis results showed cellular component (a), molecular function (b), and biological process (c). STRING analysis indicated visualization of protein-protein interactions for 20 candidate proteins (d). The notes for different color lines were in the bottom of (d). In addition, 5 candidate biomarkers verified by ELISA and Western Blot were marked with ∗ .

Article Snippet: The concentration of bradykinin, which was the downstream product of KNG1, was also detected with ELISA kit (Cusabio Biotech, Wuhan, Hubei, China).

Techniques: Protein-Protein interactions, Enzyme-linked Immunosorbent Assay, Western Blot

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Identification of Tengfu Jiangya Tablet Target Biomarkers with Quantitative Proteomic Technique

doi: 10.1155/2017/7594805

Figure Lengend Snippet: The molecular function, biological process, and expression levels of 20 candidate serum proteins quantified by iTRAQ technique.

Article Snippet: The concentration of bradykinin, which was the downstream product of KNG1, was also detected with ELISA kit (Cusabio Biotech, Wuhan, Hubei, China).

Techniques: Expressing, Multiplex sample analysis, Binding Assay, Chemotaxis Assay, Activity Assay, Antioxidant Activity Assay, Protein Binding, Transduction, Activation Assay

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Identification of Tengfu Jiangya Tablet Target Biomarkers with Quantitative Proteomic Technique

doi: 10.1155/2017/7594805

Figure Lengend Snippet: Validation of Kininogen-1, Keratin 1, Myeloperoxidase, Retinol binding protein 4, Serum amyloid A protein, and bradykinin in serum. (a) Levels of these candidate biomarkers and downstream substance were measured by ELISA in serum of EH patients ( n = 30) and TJT treated patients ( n = 30). p values were calculated with ANOVA test ( ∗ p = 0.000). (b) With Western Blot results, the expression of Kininogen 1 and Retinol binding protein 4 was significantly differentiated between two groups. Transferrin was applied as loading control.

Article Snippet: The concentration of bradykinin, which was the downstream product of KNG1, was also detected with ELISA kit (Cusabio Biotech, Wuhan, Hubei, China).

Techniques: Biomarker Discovery, Binding Assay, Enzyme-linked Immunosorbent Assay, Western Blot, Expressing, Control

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Identification of Tengfu Jiangya Tablet Target Biomarkers with Quantitative Proteomic Technique

doi: 10.1155/2017/7594805

Figure Lengend Snippet: This map directly summarized the pathway of 5 target proteins (green ovals). The arrowheads represented variation trends of target protein after TJT treatment. KNG1, KRT1, and MPO were involved in bradykinin production; meanwhile MPO and RBP4 played vital role in vascular oxidative and inflammatory injury. SAA was an acute-phase marker for vascular injury.

Article Snippet: The concentration of bradykinin, which was the downstream product of KNG1, was also detected with ELISA kit (Cusabio Biotech, Wuhan, Hubei, China).

Techniques: Marker

Journal: Genomics, Proteomics & Bioinformatics

Article Title: Integrative Proteomic Analysis of Multiple Posttranslational Modifications in Inflammatory Response

doi: 10.1016/j.gpb.2020.11.004

Figure Lengend Snippet: Degradative and non-degradative ubiquitination both exist in the LPS-stimulated ubiquitinome A. WCP ratios of five selected inflammatory factors. B. The relative protein and mRNA levels (fold change related to 0 h) for six selected genes in RAW264.7 cells with LPS stimulation. C. Abundance of ubiquitin lysine sites quantified in the Ub of LPS-stimulated macrophages. In (A–C), dada are represented as mean ± SEM ( n = 3). D. Comparison of the Log 2 M/L values of the ubiquitination sites from the LPS-stimulated Ub of cells treated with or without MG132 for 2 h. Sites exhibiting Log 2 M/L ≥ 1 in untreated and MG132-treated cells were considered dramatically affected by MG132 (highlighted in red). E. Comparison of the Log 2 M/L values of ubiquitination sites from the LPS-stimulated WCP of cells without MG132 treatment and the Ub of cells treated with MG132. Sites showing the same changes in the two proteome datasets were predicted to be non-degradative ubiquitination sites (highlighted in cyan), while the remaining sites were degradative ubiquitination sites (highlighted in red). F. Proteins in the TLR4 pathway illustrate the classification in (E).

Article Snippet: MG-132 (Catalog No. S2619, Selleck, Houston, TX), PR-619 (Catalog No. S7130, Selleck), trypsin (Catalog No. BELT001, Beierli, Tianjin, China), PTMScan Ubiquitin Remnant Motif Kit (Catalog No. 5562, Cell Signaling Technology, Bossdun, MA), PTMScan Acetyl-Lysine Motif Kit (Catalog No. 3416, Cell Signaling Technology), PTMScan Phospho-Tyrosine Rabbit mAb Kit (Catalog No. 8803, Cell Signaling Technology), High-Select Fe-NTA Phosphopeptide Enrichment Kit (Catalog No. A32992, ThermoFisher Scientific, Waltham, MA), High-Select TiO 2 Phosphopeptide Enrichment Kit (Catalog No. A32993, ThermoFisher Scientific), Protease and Phosphatase Inhibitor Cocktail (Catalog No. P1049, Beyotime, Shanghai, China), DMEM for SILAC (Catalog No. 88364, Gibco, Waltham, MA), Dialyzed Fetal Bovine Serum (Catalog No. 30067334, Gibco), L-lysine- 13 C 6 - 15 N 2 (Catalog No. 88209, Gibco), L-arginine- 13 C 6 - 15 N 4 (Catalog No. 89990, Gibco), L-lysine-4,4,5,5-D4 (Catalog No. 88438, Gibco), L-arginine- 13 C 6 (Catalog No. 88433, Gibco), and RPMI 1640 medium for SILAC (Catalog No. 88365, Gibco).

Techniques: Ubiquitin Proteomics, Comparison