multiplex pcr Search Results


green multiplex master mix  (New England Biolabs)
97
New England Biolabs green multiplex master mix
Green Multiplex Master Mix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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time assay rt qpcr  (Bio-Rad)
94
Bio-Rad time assay rt qpcr
Time Assay Rt Qpcr, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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multiplex pcr kit  (Qiagen)
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Qiagen multiplex pcr kit
Multiplex Pcr Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pcr multiplex kit  (Qiagen)
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Qiagen pcr multiplex kit
Pcr Multiplex Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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quantitect multiplex pcr kit  (Qiagen)
95
Qiagen quantitect multiplex pcr kit
Quantitect Multiplex Pcr Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hotstartaq dna polymerase  (Qiagen)
94
Qiagen hotstartaq dna polymerase
Optimization of FTA ® card processing for direct PCR amplification. FTA ® card discs inoculated with reference strain 7213384-1 at OD 600 = 0.01 producing serovar 19-specific product are shown for different optimization conditions. (A) PCR amplification from FTA ® cards using different polymerases: A = <t>HotStarTaq</t> ® Plus <t>Polymerase,</t> B = HotStarTaq ® Polymerase, and C = DreamTaq Polymerase. (B) The number of washes in “deionized water” required to achieve amplification: 0–4 = no wash to 4 washes, respectively. (C) Length of incubation with deionized water per wash: 1 = 5 min, 2 = 10 min, and 3 = 15 min. (D) Altering the volume of water used to wash in a single step for 5 min: 1 = 100 μl, 2 = 200 μl, 3 = 300 μl, 4 = 400 μl, and 5 = 500 μl. (E) Various PCR reaction volumes: 1 = 30 μl, 2 = 40 μl, and 3 = 50 μl. (F) Limit of detection is shown for amplification of the serovar 19-specific product from discs inoculated with strain 7213384-1. Lanes labelled 1–5 are for OD 600 = 0.01 and four subsequent 10-fold dilutions, respectively, of bacterial culture on discs prepared either with the Whatman FTA ® protocol (left hand lanes) or our developed wash protocol (right hand lanes). For all gels, lane M = 100 bp Plus <t>DNA</t> Ladder (GeneRuler, Thermo Fisher Scientific).
Hotstartaq Dna Polymerase, supplied by Qiagen, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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kapa2g fast multiplex pcr kit 2x  (Roche)
95
Roche kapa2g fast multiplex pcr kit 2x
Optimization of FTA ® card processing for direct PCR amplification. FTA ® card discs inoculated with reference strain 7213384-1 at OD 600 = 0.01 producing serovar 19-specific product are shown for different optimization conditions. (A) PCR amplification from FTA ® cards using different polymerases: A = <t>HotStarTaq</t> ® Plus <t>Polymerase,</t> B = HotStarTaq ® Polymerase, and C = DreamTaq Polymerase. (B) The number of washes in “deionized water” required to achieve amplification: 0–4 = no wash to 4 washes, respectively. (C) Length of incubation with deionized water per wash: 1 = 5 min, 2 = 10 min, and 3 = 15 min. (D) Altering the volume of water used to wash in a single step for 5 min: 1 = 100 μl, 2 = 200 μl, 3 = 300 μl, 4 = 400 μl, and 5 = 500 μl. (E) Various PCR reaction volumes: 1 = 30 μl, 2 = 40 μl, and 3 = 50 μl. (F) Limit of detection is shown for amplification of the serovar 19-specific product from discs inoculated with strain 7213384-1. Lanes labelled 1–5 are for OD 600 = 0.01 and four subsequent 10-fold dilutions, respectively, of bacterial culture on discs prepared either with the Whatman FTA ® protocol (left hand lanes) or our developed wash protocol (right hand lanes). For all gels, lane M = 100 bp Plus <t>DNA</t> Ladder (GeneRuler, Thermo Fisher Scientific).
Kapa2g Fast Multiplex Pcr Kit 2x, supplied by Roche, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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kapa2g fast multiplex pcr kit 2x - by Bioz Stars, 2026-07
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quantinova multiplex rt pcr kit  (Qiagen)
94
Qiagen quantinova multiplex rt pcr kit
Optimization of FTA ® card processing for direct PCR amplification. FTA ® card discs inoculated with reference strain 7213384-1 at OD 600 = 0.01 producing serovar 19-specific product are shown for different optimization conditions. (A) PCR amplification from FTA ® cards using different polymerases: A = <t>HotStarTaq</t> ® Plus <t>Polymerase,</t> B = HotStarTaq ® Polymerase, and C = DreamTaq Polymerase. (B) The number of washes in “deionized water” required to achieve amplification: 0–4 = no wash to 4 washes, respectively. (C) Length of incubation with deionized water per wash: 1 = 5 min, 2 = 10 min, and 3 = 15 min. (D) Altering the volume of water used to wash in a single step for 5 min: 1 = 100 μl, 2 = 200 μl, 3 = 300 μl, 4 = 400 μl, and 5 = 500 μl. (E) Various PCR reaction volumes: 1 = 30 μl, 2 = 40 μl, and 3 = 50 μl. (F) Limit of detection is shown for amplification of the serovar 19-specific product from discs inoculated with strain 7213384-1. Lanes labelled 1–5 are for OD 600 = 0.01 and four subsequent 10-fold dilutions, respectively, of bacterial culture on discs prepared either with the Whatman FTA ® protocol (left hand lanes) or our developed wash protocol (right hand lanes). For all gels, lane M = 100 bp Plus <t>DNA</t> Ladder (GeneRuler, Thermo Fisher Scientific).
Quantinova Multiplex Rt Pcr Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ucp multiplex pcr kit  (Qiagen)
93
Qiagen ucp multiplex pcr kit
Optimization of FTA ® card processing for direct PCR amplification. FTA ® card discs inoculated with reference strain 7213384-1 at OD 600 = 0.01 producing serovar 19-specific product are shown for different optimization conditions. (A) PCR amplification from FTA ® cards using different polymerases: A = <t>HotStarTaq</t> ® Plus <t>Polymerase,</t> B = HotStarTaq ® Polymerase, and C = DreamTaq Polymerase. (B) The number of washes in “deionized water” required to achieve amplification: 0–4 = no wash to 4 washes, respectively. (C) Length of incubation with deionized water per wash: 1 = 5 min, 2 = 10 min, and 3 = 15 min. (D) Altering the volume of water used to wash in a single step for 5 min: 1 = 100 μl, 2 = 200 μl, 3 = 300 μl, 4 = 400 μl, and 5 = 500 μl. (E) Various PCR reaction volumes: 1 = 30 μl, 2 = 40 μl, and 3 = 50 μl. (F) Limit of detection is shown for amplification of the serovar 19-specific product from discs inoculated with strain 7213384-1. Lanes labelled 1–5 are for OD 600 = 0.01 and four subsequent 10-fold dilutions, respectively, of bacterial culture on discs prepared either with the Whatman FTA ® protocol (left hand lanes) or our developed wash protocol (right hand lanes). For all gels, lane M = 100 bp Plus <t>DNA</t> Ladder (GeneRuler, Thermo Fisher Scientific).
Ucp Multiplex Pcr Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/multiplex+pcr/pmc08677670-57-8-12?v=Qiagen
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ucp multiplex pcr kit - by Bioz Stars, 2026-07
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reverse transcriptase kits  (New England Biolabs)
94
New England Biolabs reverse transcriptase kits
Optimization of FTA ® card processing for direct PCR amplification. FTA ® card discs inoculated with reference strain 7213384-1 at OD 600 = 0.01 producing serovar 19-specific product are shown for different optimization conditions. (A) PCR amplification from FTA ® cards using different polymerases: A = <t>HotStarTaq</t> ® Plus <t>Polymerase,</t> B = HotStarTaq ® Polymerase, and C = DreamTaq Polymerase. (B) The number of washes in “deionized water” required to achieve amplification: 0–4 = no wash to 4 washes, respectively. (C) Length of incubation with deionized water per wash: 1 = 5 min, 2 = 10 min, and 3 = 15 min. (D) Altering the volume of water used to wash in a single step for 5 min: 1 = 100 μl, 2 = 200 μl, 3 = 300 μl, 4 = 400 μl, and 5 = 500 μl. (E) Various PCR reaction volumes: 1 = 30 μl, 2 = 40 μl, and 3 = 50 μl. (F) Limit of detection is shown for amplification of the serovar 19-specific product from discs inoculated with strain 7213384-1. Lanes labelled 1–5 are for OD 600 = 0.01 and four subsequent 10-fold dilutions, respectively, of bacterial culture on discs prepared either with the Whatman FTA ® protocol (left hand lanes) or our developed wash protocol (right hand lanes). For all gels, lane M = 100 bp Plus <t>DNA</t> Ladder (GeneRuler, Thermo Fisher Scientific).
Reverse Transcriptase Kits, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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reverse transcriptase kits - by Bioz Stars, 2026-07
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multiplex pcr 5x master mix kit  (New England Biolabs)
97
New England Biolabs multiplex pcr 5x master mix kit
Optimization of FTA ® card processing for direct PCR amplification. FTA ® card discs inoculated with reference strain 7213384-1 at OD 600 = 0.01 producing serovar 19-specific product are shown for different optimization conditions. (A) PCR amplification from FTA ® cards using different polymerases: A = <t>HotStarTaq</t> ® Plus <t>Polymerase,</t> B = HotStarTaq ® Polymerase, and C = DreamTaq Polymerase. (B) The number of washes in “deionized water” required to achieve amplification: 0–4 = no wash to 4 washes, respectively. (C) Length of incubation with deionized water per wash: 1 = 5 min, 2 = 10 min, and 3 = 15 min. (D) Altering the volume of water used to wash in a single step for 5 min: 1 = 100 μl, 2 = 200 μl, 3 = 300 μl, 4 = 400 μl, and 5 = 500 μl. (E) Various PCR reaction volumes: 1 = 30 μl, 2 = 40 μl, and 3 = 50 μl. (F) Limit of detection is shown for amplification of the serovar 19-specific product from discs inoculated with strain 7213384-1. Lanes labelled 1–5 are for OD 600 = 0.01 and four subsequent 10-fold dilutions, respectively, of bacterial culture on discs prepared either with the Whatman FTA ® protocol (left hand lanes) or our developed wash protocol (right hand lanes). For all gels, lane M = 100 bp Plus <t>DNA</t> Ladder (GeneRuler, Thermo Fisher Scientific).
Multiplex Pcr 5x Master Mix Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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multiplex pcr 5x master mix kit - by Bioz Stars, 2026-07
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one step rt pcr kits  (New England Biolabs)
94
New England Biolabs one step rt pcr kits
Optimization of FTA ® card processing for direct PCR amplification. FTA ® card discs inoculated with reference strain 7213384-1 at OD 600 = 0.01 producing serovar 19-specific product are shown for different optimization conditions. (A) PCR amplification from FTA ® cards using different polymerases: A = <t>HotStarTaq</t> ® Plus <t>Polymerase,</t> B = HotStarTaq ® Polymerase, and C = DreamTaq Polymerase. (B) The number of washes in “deionized water” required to achieve amplification: 0–4 = no wash to 4 washes, respectively. (C) Length of incubation with deionized water per wash: 1 = 5 min, 2 = 10 min, and 3 = 15 min. (D) Altering the volume of water used to wash in a single step for 5 min: 1 = 100 μl, 2 = 200 μl, 3 = 300 μl, 4 = 400 μl, and 5 = 500 μl. (E) Various PCR reaction volumes: 1 = 30 μl, 2 = 40 μl, and 3 = 50 μl. (F) Limit of detection is shown for amplification of the serovar 19-specific product from discs inoculated with strain 7213384-1. Lanes labelled 1–5 are for OD 600 = 0.01 and four subsequent 10-fold dilutions, respectively, of bacterial culture on discs prepared either with the Whatman FTA ® protocol (left hand lanes) or our developed wash protocol (right hand lanes). For all gels, lane M = 100 bp Plus <t>DNA</t> Ladder (GeneRuler, Thermo Fisher Scientific).
One Step Rt Pcr Kits, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Optimization of FTA ® card processing for direct PCR amplification. FTA ® card discs inoculated with reference strain 7213384-1 at OD 600 = 0.01 producing serovar 19-specific product are shown for different optimization conditions. (A) PCR amplification from FTA ® cards using different polymerases: A = HotStarTaq ® Plus Polymerase, B = HotStarTaq ® Polymerase, and C = DreamTaq Polymerase. (B) The number of washes in “deionized water” required to achieve amplification: 0–4 = no wash to 4 washes, respectively. (C) Length of incubation with deionized water per wash: 1 = 5 min, 2 = 10 min, and 3 = 15 min. (D) Altering the volume of water used to wash in a single step for 5 min: 1 = 100 μl, 2 = 200 μl, 3 = 300 μl, 4 = 400 μl, and 5 = 500 μl. (E) Various PCR reaction volumes: 1 = 30 μl, 2 = 40 μl, and 3 = 50 μl. (F) Limit of detection is shown for amplification of the serovar 19-specific product from discs inoculated with strain 7213384-1. Lanes labelled 1–5 are for OD 600 = 0.01 and four subsequent 10-fold dilutions, respectively, of bacterial culture on discs prepared either with the Whatman FTA ® protocol (left hand lanes) or our developed wash protocol (right hand lanes). For all gels, lane M = 100 bp Plus DNA Ladder (GeneRuler, Thermo Fisher Scientific).

Journal: Frontiers in Veterinary Science

Article Title: Rapid Detection and Typing of Actinobacillus pleuropneumoniae Serovars Directly From Clinical Samples: Combining FTA ® Card Technology With Multiplex PCR

doi: 10.3389/fvets.2021.728660

Figure Lengend Snippet: Optimization of FTA ® card processing for direct PCR amplification. FTA ® card discs inoculated with reference strain 7213384-1 at OD 600 = 0.01 producing serovar 19-specific product are shown for different optimization conditions. (A) PCR amplification from FTA ® cards using different polymerases: A = HotStarTaq ® Plus Polymerase, B = HotStarTaq ® Polymerase, and C = DreamTaq Polymerase. (B) The number of washes in “deionized water” required to achieve amplification: 0–4 = no wash to 4 washes, respectively. (C) Length of incubation with deionized water per wash: 1 = 5 min, 2 = 10 min, and 3 = 15 min. (D) Altering the volume of water used to wash in a single step for 5 min: 1 = 100 μl, 2 = 200 μl, 3 = 300 μl, 4 = 400 μl, and 5 = 500 μl. (E) Various PCR reaction volumes: 1 = 30 μl, 2 = 40 μl, and 3 = 50 μl. (F) Limit of detection is shown for amplification of the serovar 19-specific product from discs inoculated with strain 7213384-1. Lanes labelled 1–5 are for OD 600 = 0.01 and four subsequent 10-fold dilutions, respectively, of bacterial culture on discs prepared either with the Whatman FTA ® protocol (left hand lanes) or our developed wash protocol (right hand lanes). For all gels, lane M = 100 bp Plus DNA Ladder (GeneRuler, Thermo Fisher Scientific).

Article Snippet: For optimization of FTA ® card processing conditions, single-plex PCR reactions were prepared with either a PCR kit containing HotStarTaq DNA Polymerase (Qiagen Ltd., Cat #206152) or DreamTaq HotStart DNA Polymerase (Thermo Fisher UK, Loughborough, UK, Cat #K9011) in 50 μl total reaction volumes (prepared according to manufacturers' protocols), with serovar 19-specific primers ( ) at a final concentration of 0.2 μM of each, and one prepared 3-mm sample disc per tube.

Techniques: Amplification, Incubation