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Image Search Results
Journal: Scientific Reports
Article Title: Combined knockdown of CD151 and MMP9 may inhibit the malignant biological behaviours of triple-negative breast cancer through the GSK-3β/β-catenin-related pathway
doi: 10.1038/s41598-024-71533-w
Figure Lengend Snippet: We used flow cytometry, transwell, CCK-8, and scratch wound healing assays to determine how each treatment affected TNBC cells. ( A , B ) The scratch wound healing assay was used to evaluate the migratory capacity of each cell group. ( C ) The CCK-8 assay was used to measure the ability of each cell group to proliferate. ( D , E , F ) Transwell assays were used to measure the invasion and migration potential of each cell group. ( G , H ) A flow cytometry assay was used to determine which cell groups underwent apoptosis. * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001. Note: The above experiments were performed for the shCD151 group with the shNC group as the control, the siMMP9 group with the siNC group as the control, and the shCD151 + siMMP9 group with the shCD151 group and siMMP9 group as controls.
Article Snippet: The following cells and reagents were used: the human triple-negative breast cancer cell lines MDA-MB-231 and MDA-MB-436 (Wuhan Pricella Biotechnology Co., Ltd); MDA-MB-231 cell-specific medium (L15, DMEM) (Procell Life Science & Technology); MDA-MB-436 cell-specific medium (DMEM); Lipo 8000TM transfection reagent (Beyotime, Shanghai, China); CD151 knockdown lentivirus and negative control (Obio Technology); MMP9 small interfering RNA and negative control (Obio Technology); reverse transcription kit; fluorescent quantitative PCR kit (Suzhou Novoprotein Scientific); CD151 antibody (Affinity Biosciences); CD151 antibody (Proteintech Group); MMP9 antibody (Proteintech Group); MMP9 antibody (Affinity Biosciences); p-GSK-3β antibody (Wanleibio); β-catenin antibody (Wanleibio); CCK-8 reagents (Beyotime Biosciences); and an
Techniques: Flow Cytometry, CCK-8 Assay, Wound Healing Assay, Migration, Control