Journal: Analytical Chemistry

Article Title: Reducing the Inner Filter Effect in Microplates by Increasing Absorbance? Linear Fluorescence in Highly Concentrated Fluorophore Solutions in the Presence of an Added Absorber

doi: 10.1021/acs.analchem.3c01295

Figure Lengend Snippet: Normalized uncorrected fluorescence intensity values, F 0,norm , recorded at 10 different z -positions and plotted as a function of scaled QS concentration, c norm . Top: L 1 titration in UV–vis–transparent (T) microplate. Bottom: H 1 titration in non-transparent (NT) microplate.

Article Snippet: Samples for each concentration series were prepared using the Tecan Spark M10 multimode microplate reader titrator module (Tecan, Austria).

Techniques: Fluorescence, Concentration Assay, Titration

Journal: Analytical Chemistry

Article Title: Reducing the Inner Filter Effect in Microplates by Increasing Absorbance? Linear Fluorescence in Highly Concentrated Fluorophore Solutions in the Presence of an Added Absorber

doi: 10.1021/acs.analchem.3c01295

Figure Lengend Snippet: Overview of the different IFE corrections for low fluorophore concentrations, corresponding to the results in Table (T microplates) and Table S4 (NT microplates). The values of F AD , F Z , F N , F A(best) , and F A correspond to AddAbs correction, ZINFE correction, NINFE correction, best Lakowicz correction from the whole set of z -positions, and Lakowicz correction obtained at the same z -position as F AD , respectively. Top: Normalized IFE-corrected fluorescence data for L 8 titration in T microplate. Normalized uncorrected fluorescence F 0 (L titration) is shown for comparison, and the values of F N are omitted for clarity due to the high similarity with the F Z values. Bottom: 1 – R 2 values obtained for the different types of IFE corrections in T and NT microplates. Lower 1 – R 2 values indicate better linearity.

Article Snippet: Samples for each concentration series were prepared using the Tecan Spark M10 multimode microplate reader titrator module (Tecan, Austria).

Techniques: Fluorescence, Titration, Comparison

Journal: Analytical Chemistry

Article Title: Reducing the Inner Filter Effect in Microplates by Increasing Absorbance? Linear Fluorescence in Highly Concentrated Fluorophore Solutions in the Presence of an Added Absorber

doi: 10.1021/acs.analchem.3c01295

Figure Lengend Snippet: 3D representation of the obtained 1 – R 2 results for the titrations (L 1 –L 11 ) in the T microplate. For clarity, titration L 12 was removed from the plot due to the very high concentration of added PD ( A ex,PD,1cm = 811.7). The dependent variable 1 – R 2 is a function of two independent variables: the z -position and the absorbance of the added PD. Deep purple zones indicate a region of very linear IFE corrections.

Article Snippet: Samples for each concentration series were prepared using the Tecan Spark M10 multimode microplate reader titrator module (Tecan, Austria).

Techniques: Titration, Concentration Assay