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Image Search Results
Journal: Journal of Cancer
Article Title: MUC15 is an independent prognostic factor that promotes metastases of MYCN non-amplified neuroblastoma.
doi: 10.7150/jca.89360
Figure Lengend Snippet: Figure 2. MUC15 is associated with poor prognosis in MYCN-NA NB from GEO datasets. A. The expression level of MUC15 across different INSS stages of NB (GSE45547 and GSE49710). B. Comparison of MUC15 expression between high-risk and non-high-risk NB cohorts (GSE49710). C. Differences in EFS and OS were evaluated
Article Snippet: The sections were then incubated overnight at 4°C with
Techniques: Expressing, Comparison
Journal: Journal of Cancer
Article Title: MUC15 is an independent prognostic factor that promotes metastases of MYCN non-amplified neuroblastoma.
doi: 10.7150/jca.89360
Figure Lengend Snippet: Figure 3. MUC15 is highly expressed in MYCN-NA NB. A. Expression of MUC15 in tissues with different stages. Blue and green fluorescence represent DAPI (nucleus) and MUC15, respectively. The histogram showed the relative fluorescence area (n=10). B. RT-PCR showed the MUC15 expression in patients with different stages (I+II, n=15; Ⅲ+Ⅳ, n=20). C. Western blotting revealed the ratio of MUC15 to internal reference protein in three different neuroblastoma cell lines. *p < 0.05, **p < 0.01.
Article Snippet: The sections were then incubated overnight at 4°C with
Techniques: Expressing, Fluorescence, Reverse Transcription Polymerase Chain Reaction, Western Blot
Journal: Journal of Cancer
Article Title: MUC15 is an independent prognostic factor that promotes metastases of MYCN non-amplified neuroblastoma.
doi: 10.7150/jca.89360
Figure Lengend Snippet: Figure 4. MUC15 promotes NB cell migration. A. Left: Transwell images of migration for NB cells randomly selected from each treatment group. Right: The bar chart showed the statistics of migration results of SK-N-AS and SK-N-SH. B. GESA analysis of migration-related pathways in the MYCN-NA subgroup of GSE45547. C. Western blotting showed increased expressions of three phosphorylated FAKs exposing to MUC15 overexpression and exogeneous MUC15 (20ng/ml) in SK-N-SH. D. Cytoskeletal protein staining in SK-N-AS. The green fluorescence for phalloidin combined with filamentous actin (F-actin) and excited with 488nm photoexcitation. The blue staining is DAPI for nuclear staining and MERGE as an overlapping image of two channels. SH#1&2 MUC15, knockdown of MUC15; OE-MUC15, overexpression of MUC15; MUC15, exogeneous MUC15 protein (20ng/ml). *p < 0.05, **p < 0.01, ****P<0.0001.
Article Snippet: The sections were then incubated overnight at 4°C with
Techniques: Migration, Western Blot, Over Expression, Staining, Fluorescence, Knockdown
Journal: Journal of Cancer
Article Title: MUC15 is an independent prognostic factor that promotes metastases of MYCN non-amplified neuroblastoma.
doi: 10.7150/jca.89360
Figure Lengend Snippet: Figure 5. MUC15 facilitates migration via MYCT1. A. Transcriptome analysis of MUC15 overexpression and control groups. B. MYCT1 expression situation after overexpression of MUC15 by RT-PCR in SK-N-SH (n=6). C. The content of MYCT1 after MUC15 overexpression in SK-N-SH was detected by western blotting. D. The correlation analysis between MUC15 and MYCT1 in GSE45547 and GSE49710. OE-MUC15, overexpression of MUC15; MUC15, exogeneous MUC15 protein (20ng/ml). *p < 0.05.
Article Snippet: The sections were then incubated overnight at 4°C with
Techniques: Migration, Over Expression, Control, Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot
Journal: Journal of Cancer
Article Title: MUC15 is an independent prognostic factor that promotes metastases of MYCN non-amplified neuroblastoma.
doi: 10.7150/jca.89360
Figure Lengend Snippet: Figure 6. MYCT1 rescues the MUC15-induced migration of NB. A. Transwell images of migration for NB cells were randomly selected from each group treated as indicated. B. The statistics analysis of SK-N-AS and SK-N-SH in A. C. Western blotting showed the expressions of three FAK phosphorylation in SK-N-SH treated as indicated. OE-MUC15, overexpression of MUC15; OE-(MUC15+MYCT1), overexpression of MUC15 and MYCT1; MUC15, exogeneous MUC15 protein (20ng/ml); MUC15+OE-MYCT1, exogeneous MUC15 protein (20ng/ml) and overexpression of MYCT1; OE-MYCT1, overexpression of MYCT1. ****P<0.0001.
Article Snippet: The sections were then incubated overnight at 4°C with
Techniques: Migration, Western Blot, Phospho-proteomics, Over Expression