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Image Search Results
Journal: Acta biomaterialia
Article Title: Use of hydrogel scaffolds to develop an in vitro 3D culture model of human intestinal epithelium.
doi: 10.1016/j.actbio.2017.08.035
Figure Lengend Snippet: Fig. 7. Immunohistochemistry staining (brown) of MUC2 and MUC5AC; A: monolayer and IgG as a negative control. HT29-MTX cells layered on or suspended within B: alginate, C: L-pNIPAM, and D: L-pNIPAM-co-DMAc hydrogels under static or dynamic culture conditions at a cell density of 2 106 cells/ml following 21 days. Cell nuclei were stained with haematoxylin (blue). Yellow arrows indicate positively stained cells. Scale bar = 100 mm. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
Article Snippet: Immunohistochemistrywas performed to investigate: brush border differentiation using CD10 antibody (1:100 rabbit polyclonal, enzyme antigen retrieval) (Abcam, Cambridge, UK); Zonulin 1 (ZO-1) protein expression which is a tight junction protein expressed by enterocytes using ZO-1 antibody (1:50, enzyme antigen retrieval) (Abcam, Cambridge, UK); enterocyte differentiation markers: alkaline phosphatase (ALP) antibody (1:200 rabbit polyclonal, heat antigen retrieval) (Abcam, Cambridge, UK), dipeptidyl peptidase IV (DPP IV) antibody (1:50mousemonoclonal, enzyme antigen retrieval) (Abcam, Cambridge, UK); and sucraseisomaltase antibody (SI) (1:50, mouse monoclonal antibody, heat antigen retrieval) (Santa Cruz, Heidelberg, Germany); HT29-MTX differentiation was assessed using
Techniques: Immunohistochemistry, Staining, Negative Control
Journal: Frontiers in Pharmacology
Article Title: Guishaozichuan granules can attenuate asthma in rats via the MUC5AC/EGFR signaling pathway
doi: 10.3389/fphar.2022.1011751
Figure Lengend Snippet: Effects of GSZC granules on lung MUC5AC (A) , EGFR (B) mRNA expression in rats suffering from asthma. Values are the mean ± SD, n = 6 for each group. ▲▲ p < .01 versus the normal group, ** p < .01 versus the model group.
Article Snippet:
Techniques: Expressing
Journal: Frontiers in Pharmacology
Article Title: Guishaozichuan granules can attenuate asthma in rats via the MUC5AC/EGFR signaling pathway
doi: 10.3389/fphar.2022.1011751
Figure Lengend Snippet: Effects of GSZC granules on lung MUC5AC (A) , EGFR (B) protein expression in rats suffering from asthma. Values are the mean ± SD, n = 6 for each group. ▲▲ p < .01 versus the normal group, * p < .05, ** p < .01 versus the model group.
Article Snippet:
Techniques: Expressing