Journal: Journal of Inflammation Research

Article Title: Mesenchymal Stem Cells Ameliorate DSS-Induced Experimental Colitis by Modulating the Gut Microbiota and MUC-1 Pathway

doi: 10.2147/jir.s402592

Figure Lengend Snippet: Figure 7 Ferroptosis-related genes play a role in DSS-induced colitis and MSCs reverse the process of ferroptosis. (A) The qPCR detection of MUC-1 gene in colitis tissues. (B) The immunohistochemistry images of NC, DSS+PBS, and DSS+MSC groups in colon tissues (scale bar: 100µm). (C) The CCK-8 analysis of IEC-6 cell viability. The different concentration (10, 5, and 2.5µM) of GO-203 (MUC-1 inhibitor) was used to measure the cell viability at 48 h, GO-203 10, 5, and 2.5µM vs control. (D) The proliferation level of IEC-6 cells in the presence of GO-203 (5µM) at 24 h and 48 h measured by CCK-8 assay. (E) The cell viability of IEC-6 cells in the presence of GO-203 and co-culture with MSCs. (F) The Western blot analysis of MUC-1 expression after transfected with overexpressing MUC-1 lentivirus in IEC-6 cells. (G) The proliferation level of IEC-6 cells transfected with overexpressing MUC-1 lentivirus in 24 h, 48 h, and 72 h. (H) The IEC-6 cell viability after treated with erastin and RSL3 with the comparison of control and overexpressed MUC-1 group. (I) qRT-PCR analysis of MUC-1, SLC7A11, and GPX4 after treated with GO-203 (5µM) for 48 h in IEC-6 cells. (J) qRT-PCR analysis of SLC7A11 and GPX4 after overexpressed MUC-1 in IEC-6 cells. (K) qRT-PCR analysis of SLC7A11 and GPX4 in the presence of erastin in the control and overexpressed MUC-1 group. (L) qRT-PCR analysis of SLC7A11, GPX4, and ACSL4 in the presence of RSL3 in the control and overexpressed MUC-1 group. Data are presented as mean ± SD. ***P < 0.001, **P < 0.01, *P < 0.05. Abbreviation: ns, no significance.

Article Snippet: The MUC-1 gene inhibitor GO-203 (MCE) was added to IEC-6 cells with different concentrations (10, 5, and 2.5μM).

Techniques: Immunohistochemistry, CCK-8 Assay, Concentration Assay, Control, Co-Culture Assay, Western Blot, Expressing, Transfection, Comparison, Quantitative RT-PCR

Journal: Breast cancer research and treatment

Article Title: Estrogen switches pure mucinous breast cancer to invasive lobular carcinoma with mucinous features

doi: 10.1007/s10549-012-2377-x

Figure Lengend Snippet: BCK4 cells are tumorigenic; estrogen causes a histologic switch. Images of whole tumors, grown in placebo (left MBCPure) or estrogen (E2, right MBCMixed) supplemented mice, stained with mucicarmine. Arrow denotes invasive breast carcinoma (IBC) component and arrowheads mucinous regions (MUC). Red arrow denotes stroma bisecting the IBC and MUC regions of the MBCMixed tumor

Article Snippet: Paraffin sections were stained for mucin using EZ Mucicarmine dye (Anatech Ltd.) and EZ Green (Anatech Ltd.) to counterstain nuclei according to the manufacturer’s instructions.

Techniques: Staining

Journal: Breast cancer research and treatment

Article Title: Estrogen switches pure mucinous breast cancer to invasive lobular carcinoma with mucinous features

doi: 10.1007/s10549-012-2377-x

Figure Lengend Snippet: Estrogen induces regions of mucinous and invasive breast carcinoma; bisecting region is composed of human tumor cells. ×20 images of a H&E stain and b mucicarmine stain of MBCPure tumor from placebo supplemented mouse (yellow and black arrows designate intracellular and extracellular mucin, respectively); c H&E and d mucicarmine stains of MBCMixed tumor from E2 supplemented mouse (black arrow mucinous region; asterisk invasive breast carcinoma (IBC) region; plus region bisecting mucinous and IBC; red arrow cell containing intracytoplasmic mucin); e ×10 ZsGreen stain of bisecting tumor region of E2 supplemented mouse and f ×40 ZsGreen image of bisecting tumor region

Article Snippet: Paraffin sections were stained for mucin using EZ Mucicarmine dye (Anatech Ltd.) and EZ Green (Anatech Ltd.) to counterstain nuclei according to the manufacturer’s instructions.

Techniques: Staining