Journal: Frontiers in Microbiology

Article Title: Inhibitory Effects of Lipopeptides and Glycolipids on C. albicans–Staphylococcus spp. Dual-Species Biofilms

doi: 10.3389/fmicb.2020.545654

Figure Lengend Snippet: Inhibition percentages of dual-species C. albicans – S. aureus and C. albicans – S. epidermidis biofilm formation on polystyrene in co-incubation assays. The anti-biofilm activity of BSs in soluble form was detected by CV (biofilm biomass) and MTT assays (biofilm metabolic activity).

Article Snippet: Briefly, biofilms were washed twice and, then, dipped in 0.5 ml of MTT working solution [0.075% MTT (Scharlab, Barcelona, ES) solution supplemented with 0.1% glucose (Biolife, Monza, IT) and 10 μM menadione (Sigma–Aldrich, St. Louis, MO, United States)].

Techniques: Inhibition, Activity Assay

Journal: Frontiers in Microbiology

Article Title: Inhibitory Effects of Lipopeptides and Glycolipids on C. albicans–Staphylococcus spp. Dual-Species Biofilms

doi: 10.3389/fmicb.2020.545654

Figure Lengend Snippet: Inhibition percentages of dual-species C. albicans–S. aureus and C. albicans–S. epidermidis biofilm formation on BSs treated silicone discs by CV (biofilm biomass) and MTT assays (biofilm metabolic activity).

Article Snippet: Briefly, biofilms were washed twice and, then, dipped in 0.5 ml of MTT working solution [0.075% MTT (Scharlab, Barcelona, ES) solution supplemented with 0.1% glucose (Biolife, Monza, IT) and 10 μM menadione (Sigma–Aldrich, St. Louis, MO, United States)].

Techniques: Inhibition, Activity Assay

Journal: Frontiers in Pediatrics

Article Title: Acellular foreskin dermal matrix is efficient in supporting the growth of urothelial cells derived from hypospadias patients

doi: 10.3389/fped.2025.1628435

Figure Lengend Snippet: Evaluation of cytotoxicity of foreskin ADM on primary urothelium-derived cells. MTT assay results showing the viability of primary urothelium-derived cells cultured in media conditioned with various concentrations of foreskin ADM soaking solution for 24 or 48 h. One-way ANOVA was used to assess statistical significance. “ns” denotes no significant difference.

Article Snippet: Cells were seeded into 96-well plates at a density of 1 × 104 cells per well (200 μl) and cultured for 48 h. Afterward, 10 μl MTT working solution (5 mg/ml, Yeasen) was added to each well, and the plates were incubated at 37°C for 4 h. Then, 150 μl DMSO was added to each well, followed by 10 min of agitation at 37°C.

Techniques: Derivative Assay, MTT Assay, Cell Culture