Journal: Lipids in Health and Disease

Article Title: Mevalonate inhibits acid sphingomyelinase activity, increases sphingomyelin levels and inhibits cell proliferation of HepG2 and Caco-2 cells

doi: 10.1186/s12944-015-0137-8

Figure Lengend Snippet: Cell proliferation after mevalonate treatment. Both HepG2 and Caco-2 cells were incubated with mevalonate for 48 h. The cell proliferation was determined by a kit from Trevigen, which was based on MTT reagent. Results are means ± SD from triple cultured wells in three separate experiments and expressed as percentage of the control. *** P < 0.005 compared with control

Article Snippet: The MTT Cell Proliferation Assay (MTT-CPA) kit was purchased from Trevigen (Gaithersburg, USA).

Techniques: Incubation, Cell Culture, Control

Journal: Journal of Clinical Medicine

Article Title: Characterization of the Effects of a Humanin Fragment Peptide (HNF 14 ) in Age-Related Macular Degeneration

doi: 10.3390/jcm15051686

Figure Lengend Snippet: Effects of HNF 14 on cellular metabolic activity in AMD cybrids. Cellular metabolic activity was assessed using an MTT assay in normal and age-related macular degeneration (AMD) transmitochondrial cybrid cells treated with HNF 14 (3.2 µM) for 48 h. Individual data points represent biological replicates, and horizontal bars indicate the mean ± SD ( n = 5 biological replicates). Data were normalized to untreated normal cybrids. Statistical significance is indicated as * p < 0.05, ** p < 0.01, *** p < 0.001; ns, not significant.

Article Snippet: AMD and normal cybrids plated in 96-well tissue culture plates were treated with MTT solution (Catalog number: 30006, Biotium, Fremont, CA, USA) at the 48 h time point.

Techniques: Activity Assay, MTT Assay