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MitoTracker, but not mitochondria, transfers rapidly to neurons from astrocytes (A) Mitochondria are dual labeled with outer mitochondrial membrane targeted GFP (GFP-OMM) and the MitoTracker dye. (B) Schematic diagram outlining the protocol for live co-culture experiments. Astrocytes are additionally labeled with CTDR (CellTracker Deep red) and neurons/neuronal mitochondria are identified by mTagBFP2 <t>and</t> <t>mito-mTagBFP2</t> expression, respectively. (C) Timelapse images of a dual-labeled astrocyte (central) immediately following co-culture with neurons. ( n = 3 biological repeats, 4–5 positions of interest per repeat). (D) An orthogonal view of co-cultures following timelapse acquisition (>30 min). (E) Representative image of dual-labeled astrocytes co-cultured with mito-mTagBFP2 expressing neurons for >30 min. The yellow dashed line represents the astrocyte boundary. Insert highlights that MitoTracker but not GFP-OMM labels neuronal mitochondria. See also .
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MitoTracker, but not mitochondria, transfers rapidly to neurons from astrocytes (A) Mitochondria are dual labeled with outer mitochondrial membrane targeted GFP (GFP-OMM) and the MitoTracker dye. (B) Schematic diagram outlining the protocol for live co-culture experiments. Astrocytes are additionally labeled with CTDR (CellTracker Deep red) and neurons/neuronal mitochondria are identified by mTagBFP2 <t>and</t> <t>mito-mTagBFP2</t> expression, respectively. (C) Timelapse images of a dual-labeled astrocyte (central) immediately following co-culture with neurons. ( n = 3 biological repeats, 4–5 positions of interest per repeat). (D) An orthogonal view of co-cultures following timelapse acquisition (>30 min). (E) Representative image of dual-labeled astrocytes co-cultured with mito-mTagBFP2 expressing neurons for >30 min. The yellow dashed line represents the astrocyte boundary. Insert highlights that MitoTracker but not GFP-OMM labels neuronal mitochondria. See also .
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MitoTracker, but not mitochondria, transfers rapidly to neurons from astrocytes (A) Mitochondria are dual labeled with outer mitochondrial membrane targeted GFP (GFP-OMM) and the MitoTracker dye. (B) Schematic diagram outlining the protocol for live co-culture experiments. Astrocytes are additionally labeled with CTDR (CellTracker Deep red) and neurons/neuronal mitochondria are identified by mTagBFP2 <t>and</t> <t>mito-mTagBFP2</t> expression, respectively. (C) Timelapse images of a dual-labeled astrocyte (central) immediately following co-culture with neurons. ( n = 3 biological repeats, 4–5 positions of interest per repeat). (D) An orthogonal view of co-cultures following timelapse acquisition (>30 min). (E) Representative image of dual-labeled astrocytes co-cultured with mito-mTagBFP2 expressing neurons for >30 min. The yellow dashed line represents the astrocyte boundary. Insert highlights that MitoTracker but not GFP-OMM labels neuronal mitochondria. See also .
Mtagbfp2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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MitoTracker, but not mitochondria, transfers rapidly to neurons from astrocytes (A) Mitochondria are dual labeled with outer mitochondrial membrane targeted GFP (GFP-OMM) and the MitoTracker dye. (B) Schematic diagram outlining the protocol for live co-culture experiments. Astrocytes are additionally labeled with CTDR (CellTracker Deep red) and neurons/neuronal mitochondria are identified by mTagBFP2 <t>and</t> <t>mito-mTagBFP2</t> expression, respectively. (C) Timelapse images of a dual-labeled astrocyte (central) immediately following co-culture with neurons. ( n = 3 biological repeats, 4–5 positions of interest per repeat). (D) An orthogonal view of co-cultures following timelapse acquisition (>30 min). (E) Representative image of dual-labeled astrocytes co-cultured with mito-mTagBFP2 expressing neurons for >30 min. The yellow dashed line represents the astrocyte boundary. Insert highlights that MitoTracker but not GFP-OMM labels neuronal mitochondria. See also .
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MitoTracker, but not mitochondria, transfers rapidly to neurons from astrocytes (A) Mitochondria are dual labeled with outer mitochondrial membrane targeted GFP (GFP-OMM) and the MitoTracker dye. (B) Schematic diagram outlining the protocol for live co-culture experiments. Astrocytes are additionally labeled with CTDR (CellTracker Deep red) and neurons/neuronal mitochondria are identified by mTagBFP2 <t>and</t> <t>mito-mTagBFP2</t> expression, respectively. (C) Timelapse images of a dual-labeled astrocyte (central) immediately following co-culture with neurons. ( n = 3 biological repeats, 4–5 positions of interest per repeat). (D) An orthogonal view of co-cultures following timelapse acquisition (>30 min). (E) Representative image of dual-labeled astrocytes co-cultured with mito-mTagBFP2 expressing neurons for >30 min. The yellow dashed line represents the astrocyte boundary. Insert highlights that MitoTracker but not GFP-OMM labels neuronal mitochondria. See also .
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MitoTracker, but not mitochondria, transfers rapidly to neurons from astrocytes (A) Mitochondria are dual labeled with outer mitochondrial membrane targeted GFP (GFP-OMM) and the MitoTracker dye. (B) Schematic diagram outlining the protocol for live co-culture experiments. Astrocytes are additionally labeled with CTDR (CellTracker Deep red) and neurons/neuronal mitochondria are identified by mTagBFP2 <t>and</t> <t>mito-mTagBFP2</t> expression, respectively. (C) Timelapse images of a dual-labeled astrocyte (central) immediately following co-culture with neurons. ( n = 3 biological repeats, 4–5 positions of interest per repeat). (D) An orthogonal view of co-cultures following timelapse acquisition (>30 min). (E) Representative image of dual-labeled astrocytes co-cultured with mito-mTagBFP2 expressing neurons for >30 min. The yellow dashed line represents the astrocyte boundary. Insert highlights that MitoTracker but not GFP-OMM labels neuronal mitochondria. See also .
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MitoTracker, but not mitochondria, transfers rapidly to neurons from astrocytes (A) Mitochondria are dual labeled with outer mitochondrial membrane targeted GFP (GFP-OMM) and the MitoTracker dye. (B) Schematic diagram outlining the protocol for live co-culture experiments. Astrocytes are additionally labeled with CTDR (CellTracker Deep red) and neurons/neuronal mitochondria are identified by mTagBFP2 <t>and</t> <t>mito-mTagBFP2</t> expression, respectively. (C) Timelapse images of a dual-labeled astrocyte (central) immediately following co-culture with neurons. ( n = 3 biological repeats, 4–5 positions of interest per repeat). (D) An orthogonal view of co-cultures following timelapse acquisition (>30 min). (E) Representative image of dual-labeled astrocytes co-cultured with mito-mTagBFP2 expressing neurons for >30 min. The yellow dashed line represents the astrocyte boundary. Insert highlights that MitoTracker but not GFP-OMM labels neuronal mitochondria. See also .
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MitoTracker, but not mitochondria, transfers rapidly to neurons from astrocytes (A) Mitochondria are dual labeled with outer mitochondrial membrane targeted GFP (GFP-OMM) and the MitoTracker dye. (B) Schematic diagram outlining the protocol for live co-culture experiments. Astrocytes are additionally labeled with CTDR (CellTracker Deep red) and neurons/neuronal mitochondria are identified by mTagBFP2 <t>and</t> <t>mito-mTagBFP2</t> expression, respectively. (C) Timelapse images of a dual-labeled astrocyte (central) immediately following co-culture with neurons. ( n = 3 biological repeats, 4–5 positions of interest per repeat). (D) An orthogonal view of co-cultures following timelapse acquisition (>30 min). (E) Representative image of dual-labeled astrocytes co-cultured with mito-mTagBFP2 expressing neurons for >30 min. The yellow dashed line represents the astrocyte boundary. Insert highlights that MitoTracker but not GFP-OMM labels neuronal mitochondria. See also .
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Image Search Results


MitoTracker, but not mitochondria, transfers rapidly to neurons from astrocytes (A) Mitochondria are dual labeled with outer mitochondrial membrane targeted GFP (GFP-OMM) and the MitoTracker dye. (B) Schematic diagram outlining the protocol for live co-culture experiments. Astrocytes are additionally labeled with CTDR (CellTracker Deep red) and neurons/neuronal mitochondria are identified by mTagBFP2 and mito-mTagBFP2 expression, respectively. (C) Timelapse images of a dual-labeled astrocyte (central) immediately following co-culture with neurons. ( n = 3 biological repeats, 4–5 positions of interest per repeat). (D) An orthogonal view of co-cultures following timelapse acquisition (>30 min). (E) Representative image of dual-labeled astrocytes co-cultured with mito-mTagBFP2 expressing neurons for >30 min. The yellow dashed line represents the astrocyte boundary. Insert highlights that MitoTracker but not GFP-OMM labels neuronal mitochondria. See also .

Journal: Cell Reports Methods

Article Title: MitoTracker transfers from astrocytes to neurons independently of mitochondria

doi: 10.1016/j.crmeth.2026.101338

Figure Lengend Snippet: MitoTracker, but not mitochondria, transfers rapidly to neurons from astrocytes (A) Mitochondria are dual labeled with outer mitochondrial membrane targeted GFP (GFP-OMM) and the MitoTracker dye. (B) Schematic diagram outlining the protocol for live co-culture experiments. Astrocytes are additionally labeled with CTDR (CellTracker Deep red) and neurons/neuronal mitochondria are identified by mTagBFP2 and mito-mTagBFP2 expression, respectively. (C) Timelapse images of a dual-labeled astrocyte (central) immediately following co-culture with neurons. ( n = 3 biological repeats, 4–5 positions of interest per repeat). (D) An orthogonal view of co-cultures following timelapse acquisition (>30 min). (E) Representative image of dual-labeled astrocytes co-cultured with mito-mTagBFP2 expressing neurons for >30 min. The yellow dashed line represents the astrocyte boundary. Insert highlights that MitoTracker but not GFP-OMM labels neuronal mitochondria. See also .

Article Snippet: pCAG mito-mTagBFP2 , Franck Polleux , Addgene plasmid #105011; RRID:Addgene_105011.

Techniques: Labeling, Membrane, Co-Culture Assay, Expressing, Cell Culture