Journal: The Journal of Physiology

Article Title: Intrauterine growth restriction elevates circulating acylcarnitines and suppresses fatty acid metabolism genes in the fetal sheep heart

doi: 10.1113/jp281415

Figure Lengend Snippet: Figure 9. Protein (relative to GAPDH) expression of electron transport chain subunits in growth-restricted myocardium n = 11 control, n = 12 IUGR (NDUFB8 P = 0.786, SDHB P = 0.609, UQCRC2 P = 0.964, MTCO1 P = 0.903, ATP5A P = 0.487). Means ± SD. In F: C = Control, X = IUGR, C = Calibrator Control. Numbers adjacent to ladder represent kDa.

Article Snippet: Antibodies used Protein Dilution Catalogue number Vendor RRID or reference CD36 1:1000 133625 abcam RRID:AB_2716564 ACSL1 1:1000 177958 abcam (Li et al. 2020) CPT1a 1:500 12252 Cell Signaling RRID:AB_2797857 CPT1b 1:1000 22170 ProteinTech (Abudurexiti et al. 2020) VDAC 1:1000 12454 Cell Signaling RRID:AB_2797922 ACADL 1:1000 17526 ProteinTech (Huang et al. 2014) CS 1:1000 14309 Cell Signaling RRID:AB_2665545 OXPHOS cocktail containing: NDUFB8, SDHB, UQCRC2, MTCO1, ATP5A 1:500 110413 abcam RRID:AB_2629281 GAPDH (housekeeper protein) 1:5000 47339 Novus Biologicals RRID:AB_10010294 Alpha-tubulin (housekeeper protein) 1:5000 2125 Cell Signaling RRID:AB_2619646 Anti-rabbit HRP (secondary antibody) 1:5000 7074 Cell Signaling RRID:AB_2099233 Anti-mouse HRP (secondary antibody) 1:5000 7076 Cell Signaling RRID:AB_330924 In brief, 20–30 μg of left ventricular myocardium using RIPA lysis buffer (MilliporeSigma) with a Complete Mini Protease 227 Inhibitor tablet (MilliporeSigma) and phosphatase inhibitor cocktails I and II (MilliporeSigma).

Techniques: Expressing, Control

Journal: bioRxiv

Article Title: Asiatic acid improves mitochondrial function, activates antioxidant response in the mouse brain and improves cognitive function in beta-amyloid overexpressing mice

doi: 10.1101/2024.02.21.581270

Figure Lengend Snippet: A) All four mitochondrial expressions (Mt-ND1, Mt-CYB, Mt-CO1 and Mt ATP6) in cortical synaptosomes showed statistically significant linear trends with increased AA concentration. B) All three antioxidant gene expressions (NRF2, HMOX1 and GCLC) in cortical synaptosomes showed statistically significant linear trends with increased AA concentration.

Article Snippet: Relative gene expression was determined using TaqMan Gene Expression Master Mix (Invitrogen) and commercially available TaqMan primers (Invitrogen) for synaptophysin (Mm00436850_m1), post-synaptic density protein 95 (PSD95; Mm00492193_m1), NRF2 (Mm00477784_m1), glutamate-cysteine ligase catalytic subunit (GCLC; Mm00802655_m1), heme oxygenase 1 (HMOX1; Mm00516005_m1), mitochondrially encoded NADH:Ubiquinone Oxidoreductase Core Subunit 1(Mt-ND1; Hs02596873_s1), mitochondrially Encoded Cytochrome B (Mt-CYB; Hs02596867_s1), Mitochondrially Encoded Cytochrome C Oxidase I (Mt-CO1; Hs02596864_g1), Mitochondrially Encoded ATP Synthase Membrane Subunit 6 (Mt-ATP6; Hs02596862_g1) and glyceraldehyde-3phosphate dehydrogenase (GAPDH; Hs02758991_g1).

Techniques: Concentration Assay

Journal: bioRxiv

Article Title: Asiatic acid improves mitochondrial function, activates antioxidant response in the mouse brain and improves cognitive function in beta-amyloid overexpressing mice

doi: 10.1101/2024.02.21.581270

Figure Lengend Snippet: ETC gene expression in cortical synaptosomes was quantified in female (A-D) and male (E-H) mice. AA treatment increased expression of Mt-CYB (B), Mt-CO1 (C) and Mt-ATP6 (D) in female 5xFAD mice. In male mice AA treatment only significantly increase expression of Mt-CO1 (G). *p<0.05, **p<0.01

Article Snippet: Relative gene expression was determined using TaqMan Gene Expression Master Mix (Invitrogen) and commercially available TaqMan primers (Invitrogen) for synaptophysin (Mm00436850_m1), post-synaptic density protein 95 (PSD95; Mm00492193_m1), NRF2 (Mm00477784_m1), glutamate-cysteine ligase catalytic subunit (GCLC; Mm00802655_m1), heme oxygenase 1 (HMOX1; Mm00516005_m1), mitochondrially encoded NADH:Ubiquinone Oxidoreductase Core Subunit 1(Mt-ND1; Hs02596873_s1), mitochondrially Encoded Cytochrome B (Mt-CYB; Hs02596867_s1), Mitochondrially Encoded Cytochrome C Oxidase I (Mt-CO1; Hs02596864_g1), Mitochondrially Encoded ATP Synthase Membrane Subunit 6 (Mt-ATP6; Hs02596862_g1) and glyceraldehyde-3phosphate dehydrogenase (GAPDH; Hs02758991_g1).

Techniques: Gene Expression, Expressing