Journal: Antibiotics

Article Title: Heterogeneity of Biofilm Formation Among Staphylococcus aureus and Coagulase-Negative Staphylococcus Species in Clinically Relevant Intravenous Fat Emulsions

doi: 10.3390/antibiotics14050484

Figure Lengend Snippet: Biofilm formation of select Staphylococcus aureus isolates in varying concentrations of SO-IFE, MO-IFE, and FO-IFE. Representative isolates of four categories of S. aureus : methicillin-susceptible S. aureus (MSSA), methicillin-resistant S. aureus (MRSA) clade USA 100, MRSA clade USA 300, and vancomycin-resistant S. aureus (VRSA) were cultivated as biofilms for 24 h in 0.5× tryptic soy broth (TSB) supplemented with serial dilutions of soybean oil intravenous fat emulsion (SO-IFE), mixed-oil intravenous fat emulsion (MO-IFE), or fish oil intravenous fat emulsion (FO-IFE). Data expressed as mean ± standard deviation. N = 2 replicates. *, p < 0.05; **, p < 0.01; ***, p < 0.001 using two-way ANOVA and Dunnett’s multiple comparison post-test to SO-IFE at each concentration.

Article Snippet: This included 5 isolates each of methicillin-susceptible S. aureus (MSSA), methicillin-resistant S. aureus (MRSA) clade USA100, MRSA clade USA300, vancomycin-resistant S. aureus (VRSA), S. capitis , S. epidermidis , S. haemolyticus , S. hominis , and S. lugdunensis that were obtained from BEI Resources or the American Type Culture Collection.

Techniques: Emulsion, Standard Deviation, Comparison, Concentration Assay

Journal: Antibiotics

Article Title: Heterogeneity of Biofilm Formation Among Staphylococcus aureus and Coagulase-Negative Staphylococcus Species in Clinically Relevant Intravenous Fat Emulsions

doi: 10.3390/antibiotics14050484

Figure Lengend Snippet: Biofilm formation of an expanded panel of Staphylococcus aureus isolates in clinically relevant concentrations of SO-IFE, MO-IFE, and FO-IFE. Laboratory or clinical isolates of S. aureus categorized as methicillin-susceptible S. aureus (MSSA), methicillin-resistant S. aureus (MRSA) clade USA 100, MRSA clade USA 300, and vancomycin-resistant S. aureus (VRSA) were cultivated as biofilms for 24 h in 0.5× tryptic soy broth supplemented with 5% soybean oil intravenous fat emulsion (SO-IFE), mixed-oil intravenous fat emulsion (MO-IFE), or fish oil intravenous fat emulsion (FO-IFE). Data expressed as mean ± standard deviation. N = 2 replicates. *, p < 0.05; **, p < 0.01; ***, p < 0.001 using two-way ANOVA and Dunnett’s multiple comparison post-test.

Article Snippet: This included 5 isolates each of methicillin-susceptible S. aureus (MSSA), methicillin-resistant S. aureus (MRSA) clade USA100, MRSA clade USA300, vancomycin-resistant S. aureus (VRSA), S. capitis , S. epidermidis , S. haemolyticus , S. hominis , and S. lugdunensis that were obtained from BEI Resources or the American Type Culture Collection.

Techniques: Emulsion, Standard Deviation, Comparison

Journal: Antibiotics

Article Title: Heterogeneity of Biofilm Formation Among Staphylococcus aureus and Coagulase-Negative Staphylococcus Species in Clinically Relevant Intravenous Fat Emulsions

doi: 10.3390/antibiotics14050484

Figure Lengend Snippet: Impact of IFEs on Staphylococcus aureus planktonic growth. Planktonic cultures of laboratory or clinical isolates of S. aureus categorized as methicillin-susceptible S. aureus (MSSA), methicillin-resistant S. aureus (MRSA) clade USA 100, MRSA clade USA 300, and vancomycin-resistant S. aureus (VRSA) were grown in 0.5× tryptic soy broth (TSB) supplemented with 10% SO-IFE (soybean oil IFE), MO-IFE (mixed-oil IFE), or 5% FO-IFE (fish oil IFE) and subsequently enumerated by drop plate method. Data expressed as mean and standard deviation. N = 2 replicates. *, p < 0.05; **, p < 0.01; ***, p < 0.001 using a two-way ANOVA and Dunnett’s multiple comparison post-test.

Article Snippet: This included 5 isolates each of methicillin-susceptible S. aureus (MSSA), methicillin-resistant S. aureus (MRSA) clade USA100, MRSA clade USA300, vancomycin-resistant S. aureus (VRSA), S. capitis , S. epidermidis , S. haemolyticus , S. hominis , and S. lugdunensis that were obtained from BEI Resources or the American Type Culture Collection.

Techniques: Standard Deviation, Comparison

Journal: Frontiers in Microbiology

Article Title: Laboratory-Based and Point-of-Care Testing for MSSA/MRSA Detection in the Age of Whole Genome Sequencing

doi: 10.3389/fmicb.2018.01437

Figure Lengend Snippet: Global review of future and commercial PCR tests for meticillin-resistant and -susceptible strains of Staphylococcus aureus .

Article Snippet: Atlas Genetics, United Kingdom , In development , Mixed technology linking NAT and immunology for MRSA, Dual MRSA/MSSA , 0.5 h.

Techniques: In Situ Hybridization, Western Blot, Amplification, Multiplex Assay, Real-time Polymerase Chain Reaction, Reverse Transcription Polymerase Chain Reaction, Binding Assay, DNA Hybridization, Hybridization, Nucleic Acid-based Assay, DNA Extraction, Sequencing

Journal: Annals of Clinical Microbiology and Antimicrobials

Article Title: Molecular characterization of methicillin-resistant Staphylococcus aureus among insulin-dependent diabetic individuals in Brazil

doi: 10.1186/s12941-020-00401-y

Figure Lengend Snippet: Dendrogram of the PFGE- SmaI and PFGE- ApaI profiles of MRSA isolated from insulin-dependent diabetic individuals generated by Dice analysis/UPGMA (BioNumerics, Applied Maths) and their molecular characterization by SCC mec typing and MLST. a. Isolates showing > 80% similarity ( clusters A and B) after digestion with SmaI . b. Band pattern of strain 735 N obtained by digestion with ApaI . N, nasal mucosa; O, oropharyngeal mucosa; S, susceptible; R, resistant. * International clones used as controls

Article Snippet: Fig. 3 Dendrogram of the PFGE- ApaI profiles of MSSA isolates generated by Dice analysis/UPGMA (BioNumerics, Applied Maths) and sequence types obtained by MLST.

Techniques: Isolation, Generated, Clone Assay

Journal: Annals of Clinical Microbiology and Antimicrobials

Article Title: Molecular characterization of methicillin-resistant Staphylococcus aureus among insulin-dependent diabetic individuals in Brazil

doi: 10.1186/s12941-020-00401-y

Figure Lengend Snippet: Dendrogram of the PFGE- ApaI profiles of MSSA isolates generated by Dice analysis/UPGMA (BioNumerics, Applied Maths) and sequence types obtained by MLST. Clustering of isolates digested with ApaI that were analyzed by MLST. All isolates except for 735 N were susceptible to methicillin (MSSA). N, nasal mucosa; O, oropharyngeal mucosa; arc C, carbamate kinase; aro E, shikimate dehydrogenase; glp F, glycerol kinase; gmk , guanylate kinase; pta , phosphate acetyltransferase; tpi , triosephosphate isomerase; yqi L, acetyl coenzyme A; ST, sequence type. Isolate 76 N was identified as ST398 in a previous study from our group. Isolate 700O was sent to the curator of the MLST database ( https://pubmlst.org/ ) for identification of the new ST. This isolate was identified as ST 6133

Article Snippet: Fig. 3 Dendrogram of the PFGE- ApaI profiles of MSSA isolates generated by Dice analysis/UPGMA (BioNumerics, Applied Maths) and sequence types obtained by MLST.

Techniques: Generated, Sequencing

Journal: BMC Complementary and Alternative Medicine

Article Title: Anti-microbial activities of pomegranate rind extracts: enhancement by cupric sulphate against clinical isolates of S. aureus , MRSA and PVL positive CA-MSSA

doi: 10.1186/1472-6882-9-23

Figure Lengend Snippet: Antibiotic resistances profile for the S. aureus tested.

Article Snippet: The MRSA and MSSA isolates were collected from the Royal Marsden hospital (London, UK) and the PVL positive CA-MSSA isolates were collected from the Devon and Exeter hospital (UK).

Techniques: