Journal: Cell reports

Article Title: Inhibiting G6PD by quercetin promotes degradation of EGFR T790M mutation.

doi: 10.1016/j.celrep.2023.113417

Figure Lengend Snippet: Figure 3. Quercetin induces M790 oxidation for EGFRT790M degradation through impairing NADPH-dependent MsrA activity (A) FLAG-tagged EGFRT790M immunoprecipitated from HEK293T cells was analyzed by Matrix-Assisted Laser Desorption Ionization Tandem Time-of-Flight Mass Spectrometry (MALDI-TOF/TOF MS). M790 residue was identified to be oxidized. See also Table S3. (B) H820 and H1975 cells were transfected with or without G6PD siRNA for 48 h. (C) H820 and H1975 cells overexpressing escalating FLAG-G6PD were treated with or without 20 mM Que for 48 h. (D) H820 and H1975 cells overexpressed with or without FLAG-ME1 or FLAG-IDH1 were treated with or without 20 mM Que for 48 h. (E) H820 and H1975 cells were treated with or without 20 mM Que or 20 mM NADPH supplementation for 48 h. (F) H820 and H1975 cells with or without MsrA depletion were treated with or without 20 mM Que for 48 h. (G) H820 and H1975 cells with or without MsrA depletion were treated with or without 20 mM NADPH supplementation for 24 h. (H–J) H820 and H1975 cells incubated with or without Ox4 were treated with or without 20 mM Que for 24 h (H) or depleted with or without siRNAs targeting G6PD (I) or MsrA (J). ReACT assay was performed to detect the levels of methionine oxidation in EGFR. (B–H) Immunoprecipitation and immunoblotting analyses were performed with the indicated antibodies. See also Figure S4.

Article Snippet: G6PD siRNA, ME1 siRNA and MsrA siRNA were from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

Techniques: Activity Assay, Immunoprecipitation, Mass Spectrometry, Residue, Transfection, Incubation, Western Blot

Journal: International Journal of Molecular Sciences

Article Title: The Interaction of Selective A1 and A2A Adenosine Receptor Antagonists with Magnesium and Zinc Ions in Mice: Behavioural, Biochemical and Molecular Studies

doi: 10.3390/ijms22041840

Figure Lengend Snippet: Effect of co-administration of DPCPX and istradefylline with Mg 2+ and Zn 2+ on gene expression ( A ) Adora1 , ( B ) Slc6a15 , ( C ) Comt , ( D ) Ogg1 , ( E ) MsrA , and ( F ) Nrf2 in mice prefrontal cortex. DPCPX, Mg 2+ and saline were administered i.p. 30 min, whereas istradefylline p.o. and Zn 2+ i.p. 60 min prior decapitation. The data are presented as the means ± SEM. Each experimental group consisted of 10 animals. ( A ) * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 vs. NaCl-treated group; ^^^^ p < 0.0001 vs. DPCPX-treated group; ### p < 0.001 vs. istradefylline-treated group; ++++ p < 0.0001 vs. Mg 2+ -treated group; &&&& p < 0.0001 vs. Zn 2+ -treated group (two-way ANOVA followed by Bonferroni’s post hoc test). ( B ) * p < 0.05, **** p < 0.0001 vs. NaCl-treated group; #### p < 0.0001 vs. istradefylline-treated group; &&&& p < 0.0001 vs. Zn 2+ -treated group (two-way ANOVA followed by Bonferroni’s post hoc test). ( C ) * p < 0.05, ** p < 0.01, **** p < 0.0001 vs. NaCl-treated group; ^^^ p < 0.001, ^^^^ p < 0.0001 vs. DPCPX-treated group; # p < 0.05, ## p < 0.01 vs. istradefylline-treated group; ++++ p < 0.0001 vs. Mg 2+ -treated group; &&& p < 0.001, &&&& p < 0.0001 vs. Zn 2+ -treated group (two-way ANOVA followed by Bonferroni’s post hoc test). ( D ) *** p < 0.001, **** p < 0.0001 vs. NaCl-treated group; ^^^^ p < 0.0001 vs. DPCPX-treated group; # p < 0.05, ### p < 0.001 vs. istradefylline-treated group; + p < 0.05 vs. Mg 2+ -treated group (two-way ANOVA followed by Bonferroni’s post hoc test). ( E ) * p < 0.05, ** p < 0.01 vs. NaCl-treated group; #### p < 0.0001 vs. istradefylline-treated group; + p < 0.05, +++ p < 0.001 vs. Mg 2+ -treated group; && p < 0.01 vs. Zn 2+ -treated group (two-way ANOVA followed by Bonferroni’s post hoc test). ( F ) *** p < 0.001, **** p < 0.0001 vs. NaCl-treated group; ^^^^ p < 0.0001 vs. DPCPX- treated group; # p < 0.05 vs. istradefylline-treated group; ++++ p < 0.0001 vs. Mg 2+ -treated group; &&& p < 0.001, &&&& p < 0.0001 vs. Zn 2+ -treated group (two-way ANOVA followed by Bonferroni’s post hoc test).

Article Snippet: Msra , Methionine sulfoxide reductase A , NM_001253712.1 NM_001253714.1 NM_001253716.1 NM_026322.4 , Mm00452737_m1 , 69.

Techniques: Gene Expression, Saline

Journal: International Journal of Molecular Sciences

Article Title: The Interaction of Selective A1 and A2A Adenosine Receptor Antagonists with Magnesium and Zinc Ions in Mice: Behavioural, Biochemical and Molecular Studies

doi: 10.3390/ijms22041840

Figure Lengend Snippet: Gene symbols, gene names, GenBank reference sequence accession numbers, assay IDs and amplicon lengths (bp).

Article Snippet: Msra , Methionine sulfoxide reductase A , NM_001253712.1 NM_001253714.1 NM_001253716.1 NM_026322.4 , Mm00452737_m1 , 69.

Techniques: Sequencing, Amplification, Derivative Assay, Binding Assay

Journal: Lipids in Health and Disease

Article Title: Tetradecylthiopropionic acid induces hepatic mitochondrial dysfunction and steatosis, accompanied by increased plasma homocysteine in mice

doi: 10.1186/s12944-016-0192-9

Figure Lengend Snippet: Hepatic gene expression levels a

Article Snippet: Real-time PCR was performed with Sarstedt 384 well multiply-PCR Plates (Sarstedt Inc., Newton, NC, USA) on the following genes, using probes and primers from Applied Biosystems: Chka (Mm00442759_m1), Chkb (Mm04213225_s1), Chdh (Mm00549261_m1), Bhmt (Mm04210521_g1), Dmgdh (Mm00512022_m1), Sardh (Mm00454657_m1), Shmt1 (Mm00486110_m1), Shmt2 (Mm00659512_g1), Gnmt (Mm00494688_m1), Mtr (Mm01340053_m1), Mtrr _(Mm00549978_m1), Msra (Mm00452738_m1), Msrb2 (Mm00512937_m1), Cbs (Mm00460654_m1), Cth (Mm00461247_m1), Ppargc1a (Mm00447183), Pparg (Mm00440945), Ppara (Mm00440939), CD36 / Fat (Mm00432403), Fabp1 (Mm00444340), Lipe (Mm00495359), Acly (Mm01302282_m1), Slc25a20 (Mm00451571_m1), Lipc (Mm00433975), Apob (Mm01545156_m1), IL1β (Mm01336189_m1), Sod1 (Mm01344233_m1), Sod2 (Mm01313000_m1), Tnfα (Mm00443258_m1) and Pcyt1a (Mm00447774_m1).

Techniques: Gene Expression, Control, Binding Assay, Antioxidant Activity Assay