Journal: PLoS ONE

Article Title: Pharmacological Blockade of Cannabinoid CB 1 Receptors in Diet-Induced Obesity Regulates Mitochondrial Dihydrolipoamide Dehydrogenase in Muscle

doi: 10.1371/journal.pone.0145244

Figure Lengend Snippet: Primer References for TaqMan® Gene Expression Assays (ThermoFisher).

Article Snippet: Eno3 , Rn01464911_m1 , 77.

Techniques: Gene Expression, Amplification

Journal: PLoS ONE

Article Title: Pharmacological Blockade of Cannabinoid CB 1 Receptors in Diet-Induced Obesity Regulates Mitochondrial Dihydrolipoamide Dehydrogenase in Muscle

doi: 10.1371/journal.pone.0145244

Figure Lengend Snippet: Proteins Identified using 2D Electrophoresis and MALDI-TOF/LC-ESI MS Analyses.

Article Snippet: Eno3 , Rn01464911_m1 , 77.

Techniques: Two-Dimensional Gel Electrophoresis

Journal: PLoS ONE

Article Title: Pharmacological Blockade of Cannabinoid CB 1 Receptors in Diet-Induced Obesity Regulates Mitochondrial Dihydrolipoamide Dehydrogenase in Muscle

doi: 10.1371/journal.pone.0145244

Figure Lengend Snippet: AM251 effects on the protein expressions of GPI, TPI, Eno3, PKM1, LDHa and Glo1 in the abdominal muscle of STD, HFD and HCD-fed rats ( A-F ). Representative 2D polyacrylamide gels showing the intensity of the identified spots ( G ). Bonferroni’s test ( n = 6): * P <0.05, ** P <0.01, *** P <0.001 vs . STD-vehicle; # P <0.05, ## P <0.01, ### P <0.001 vs . HFD-vehicle; $ P <0.05, $ $ $ P <0.001 vs . HCD-vehicle.

Article Snippet: Eno3 , Rn01464911_m1 , 77.

Techniques:

Journal: PLoS ONE

Article Title: Pharmacological Blockade of Cannabinoid CB 1 Receptors in Diet-Induced Obesity Regulates Mitochondrial Dihydrolipoamide Dehydrogenase in Muscle

doi: 10.1371/journal.pone.0145244

Figure Lengend Snippet: AM251 effects on the gene expressions of Gpi , Tpi1 , Eno3 , Pkm , Ldha and Glo1 in the abdominal muscle of STD, HFD and HCD-fed rats. Bonferroni’s test ( n = 8): * P <0.05, *** P <0.001 vs . STD-vehicle; $ P <0.05 vs . HCD-vehicle.

Article Snippet: Eno3 , Rn01464911_m1 , 77.

Techniques:

Journal: PLoS ONE

Article Title: Pharmacological Blockade of Cannabinoid CB 1 Receptors in Diet-Induced Obesity Regulates Mitochondrial Dihydrolipoamide Dehydrogenase in Muscle

doi: 10.1371/journal.pone.0145244

Figure Lengend Snippet: Gene ( A ) and protein ( B ) expressions of GPI, TPI, Eno3, PKM1, LDHa and Glo1 in the abdominal muscle of wild-type and CB 1 -/- mice. C) Representative immunoblots. Student’s t test ( n = 6): * P <0.05 vs . WT.

Article Snippet: Eno3 , Rn01464911_m1 , 77.

Techniques: Western Blot

Journal: Cell Reports Medicine

Article Title: Non-orthosteric inhibition of enolase 1 impedes growth of triple-negative breast cancer

doi: 10.1016/j.xcrm.2025.102451

Figure Lengend Snippet: SU212 targets ENO1 (A) Chemical structure of podophyllotoxin (parental compound) and SU212. (B–D) SU212 binds to ENO1 and ENO3. Target identification using CETSA: Differential profiling of SU212 on the thermal proteome profile of MDA-MB-231 cells. Cells were treated with DMSO or SU212 (0.5 μM) for 1.5 h and then lysed, and an equal quantity of soluble protein was labeled with a tandem mass tag, followed by liquid chromatography-tandem mass spectrometry analysis. (B) Heatmap representation of the thermal stability of 1,074 soluble proteins in cancer cells treated with vehicle-DMSO (left) and SU212 (right). (C) A scatterplot of melting temperature (T m ) calculated after SU212 and vehicle treatment. Proteins that passed the significant value thresholds ( p < 0.01, R2 > 0.8) and identification criteria are highlighted in orange. (D) Melting curves for ENO1/ENO3 with and without SU212 treatment depict the change in T m . (E) Representative sensorgrams for ENO1/3-SU212 interaction. His-tagged ENO1 and ENO3 proteins were immobilized on a Ni-NTA sensor, and SU212 (10 μM) was tested for physical interaction using BLI. (F) SU212 physically interacts with ENO1 protein. The BLI sensorgrams were obtained using His tag-ENO1-loaded Octet NTA biosensors and SU212 (1, 5, 10 μM). (G) SU212 treatment inhibits ENO1 expression. Immunoblotting: TNBC cells were treated with vehicle only (DMSO) and SU212 (0.1, 0.25, 0.5 μM) for 6 h. SDS-PAGE and western blot analysis were performed for the ENO1 and ENO3 proteins. Membranes were stripped and re-probed with anti-beta-actin antibody to ensure equal protein loading. (H) SU212 treatment leads to degradation of the ENO1 protein. Immunoblotting: MDA-MB-231 cells were treated with combinations of SU212, CHX, MG132, 3MA, and NH4Cl, as depicted in the figure, for 6 h. SDS-PAGE and western blot analysis were performed for the ENO1 protein. Membranes were stripped and re-probed with anti-GAPDH antibody to ensure equal protein loading. (I) SU212 treatment induces apoptotic cell death in MDA-MB-231 cells. Data are shown as the mean ± SD ( n = 5). Numbers indicate a p value compared with the vehicle control and analyzed using two-way ANOVA. (J) Enolase enzyme activity assay. PC, positive control. Data are shown as the mean ± SD ( n = 3). Numbers indicate a p value that is different compared with vehicle control, analyzed using Student’s t test. ns, not significant.

Article Snippet: During the loading step, recombinant His Tag-Enolases were immobilized on NTA Biosensors using a kinetic buffer (1X PBS) with a final reagent volume of 50 μL, which contained 50 μg/mL of ENO1 (#11554-H07E−100, Sino Biological) and ENO3 (#14270-H07E, Sino Biological), all placed in a black 384-well microplate.

Techniques: Drug discovery, Labeling, Liquid Chromatography, Mass Spectrometry, Expressing, Western Blot, SDS Page, Control, Enzyme Activity Assay, Positive Control

Journal: Stem Cell Research & Therapy

Article Title: Pax7 as molecular switch regulating early and advanced stages of myogenic mouse ESC differentiation in teratomas

doi: 10.1186/s13287-020-01742-3

Figure Lengend Snippet: Selected gene expression in teratomas obtained from Pax7+/+ and Pax7−/− ESCs. a Expression of mRNAs encoding pluripotency marker ( Nanog ), marker of primitive streak—mesodermal cell marker ( Brachyury ), and markers of myogenic cells ( Myod1 , MyoG ) (for each genotype n = 7). b Expression of mRNAs encoding embryonic myoblast markers ( Pax3 , Nfatc4 ) (for each genotype n = 7). c Expression of mRNAs encoding fetal myoblast markers ( Nfix , Eno3 , Mck , Mef2a , Itga7 ) (for each genotype n = 5 to n = 9). Expression was related to the levels observed in 13.5 d.p.c. mouse embryo (E13.5; n = 3), and normalized to mRNA encoding β-actin ( Actb ). d – g Western blot analysis of Pax3, MyoD, myogenin, and Mck in teratomas obtained from three Pax7+/+ ESC lines and three Pax7−/− ESC lines (for each genotype n = 3). Hsp90 level was used as a loading control. Graph represents optical density of Pax3, MyoD, Myogenin, and Mck bands compared to density of corresponding Hsp90 bands (optical density of Hsp90 was accounted as 100%, OdU optical density, arbitrary units). White bar—values for Pax7+/+ teratomas, gray bar—values for Pax7−/− teratomas. Data characterized by non-normal distribution ( c , Eno3 ) was normalized. Data are presented as mean ± SD. Stars represent results of Student’s unpaired two-tailed t test: * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001

Article Snippet: mRNA was isolated from teratomas, using mirVana Isolation Kit (Thermo Fisher Scientific). mRNA analysis reverse transcription was performed using 1 μg total RNA and RevertAid First Strand cDNA Synthesis Kit (Thermo Fisher Scientific) according to the manufacturer’s instruction. qPCR was performed using specific TaqMan® probes: Mm02019550_s1 ( Nanog ), Mm00435493_m1 ( Pax3 ), Mm00435125_m1 ( Myf5 ), Mm00440387_m1 ( Myod1 ), Mm00446194_m1 ( MyoG ), Mm00483191_m1 ( Cdh15 ), Mm01318252_m1 ( T encoding Brachyury), Mm00477791_m1 ( Nfix ), Mm00468267_m1 ( Eno3 ), Mm01321487_m1 ( Mck ), Mm00434400_m1 ( Itga7 ), Mm01318991_m1 ( Mef2a ), Mm00452375_m1 ( Nfatc4 ), Mm01332463_m1 ( Myh3 ), Mm01332564_m1 ( Myh2 ), Mm01319006_g1 ( Myh7 ), Mm01332541_m1 ( Myh4 ), Mm01226102_m1 ( Lama1 ), Mm00493080_m1 ( Lamb2 ), Mm01193660_m1 ( Lama4 ), Mm01222010_g1 ( Lama5 ), Mm01248771_m1 ( RbFox3 ), Mm00446859_m1 ( Otx2 ), Mm01205647_g1 ( Actb ), and the TaqMan Gene Expression Master Mix (Thermo Fisher Scientific).

Techniques: Gene Expression, Expressing, Marker, Western Blot, Control, Two Tailed Test

Journal: PLoS ONE

Article Title: Transcriptome, Methylome and Genomic Variations Analysis of Ectopic Thyroid Glands

doi: 10.1371/journal.pone.0013420

Figure Lengend Snippet: Validated genes (n = 19) with convergent induced (n = 16) and repressed (n = 3) expression in ectopic thyroid tissue (i.e. independent of the activation state and dependent on the localization of the thyroid tissue).

Article Snippet: ENO3 , 2027 , −1,99 , −0,86 , −1,15 , Hs00266551_m1.

Techniques: Expressing, Activation Assay

Journal: eLife

Article Title: Screening identifies small molecules that enhance the maturation of human pluripotent stem cell-derived myotubes

doi: 10.7554/eLife.47970

Figure Lengend Snippet:

Article Snippet: Following are the taqman probes used in this study, MYH1 (Hs00428600_m1), MYH2 (Hs00430042_m1), MYH3 (Hs01074230_m1), MYH7 (Hs01110632_m1), MYH8 (Hs00267293_m1), MYOG (Hs01072232_m1), MYOD1 (Hs02330075_g1), ACTB (Hs99999903_m1), GAPDH (Hs99999905_m1), SLN (Hs00161903_m1), CAPN3 (Hs01115989_m1), ATP2A1 (Hs01115989_m1), ENO3 (Hs01093275_m1), MYF6 (Hs00231165_m1), CKM (Hs00176490_m1), KLF4 (Hs01034973_g1), TNNT3 (Hs00952980_m1), CDH11 (Hs00901479_m1), EYA2 (Hs00193347_m1), FST (Hs01121165_g1), CEBPB (Hs00270923_s1) , CEBPD (Hs00270931_s1), FKBP5 (Hs01561006_m1), NOTCH2 (Hs01050702_m1) , HES1 (Hs00172878_m1), JAG1 (Hs01070032_m1) , COL1A1 (Hs00164004_m1), ID3 (Hs00954037_g1) , SERPINE1 (Hs00167155_m1), PPARGC1A (Hs00173304_m1) , RGS2 (Hs01009070_g1), NR4A1 (Hs00374226_m1).

Techniques: Control, Recombinant, Imaging, Proliferation Assay, Sequencing

Journal: bioRxiv

Article Title: Proteogenomic single cell analysis of skeletal muscle myocytes

doi: 10.1101/2020.01.23.916791

Figure Lengend Snippet: a) HPA images (top row) are mosaic for DCAF11 and ENO3 and negative for PVALB staining. Follow up staining validated the DCAF11 and ENO3 staining while suggesting a subtle mosaicism of PVALB in humans. In mice, ENO3 and PVALB are clearly mosaic, while DCAF11 is not. b) RNA-ISH demonstrates co-expression of CYB5R1 and ENO3 in a mosaic pattern. c) Only Eno3 was observed (in a mosaic pattern) in mouse muscle by RNA-ISH.

Article Snippet: Antibodies were obtained for WDR23/DCAF11 (bs-8388R, Bioss Antibodies), PVALB (A2781, Abclonal), and ENO3 (ARP48203_T100, Aviva Systems Biology) that were reported to cross react to both human and mouse.

Techniques: Staining, Expressing