mscv Search Results


addgene plasmid  (Addgene inc)
94
Addgene inc addgene plasmid
Addgene Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ires gfp sequence  (Addgene inc)
93
Addgene inc ires gfp sequence
Ires Gfp Sequence, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rack1  (Addgene inc)
93
Addgene inc rack1
Fig. 4 | AIM2 promotes Treg cells in vitro and restrains AKT–mTOR via the <t>RACK1–PP2A</t> complex. a, b, RT–PCR (a) and flow cytometry (b) of FOXP3 in wild-type and Aim2−/− CD4+ T cells activated with the indicated amounts (a) or 2 ng ml−1 (b) of TGFβ for 4 days; n = 5 experiments in a and n = 4 experiments in b. c, Flow cytometry of IFNγ+ or IL-17A+ CD4+ T cells of indicated genotypes, 4 days after differentiation under indicated polarizing conditions; n = 4 experiments. cTH17, classic TH17; pTH17, pathogenic TH17. d–g, Wild-type and Aim2−/− CD4+ T cells were stimulated as in b. d, Enrichment scores of indicated gene sets, based on RNA-seq datasets. e, f, ECAR (e) and OCR (f) levels during glycolysis, and OCR levels during fatty acid oxidation (g), by Seahorse analysis; n = 10 experiments for e; n = 5 experiments for f; and n = 3 experiments for g. h, Immunoblotting of indicated proteins in wild-type and Aim2−/− CD4+ T cells stimulated as in b with indicated treatment for 24 h. Representative of three
Rack1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rack1/product/Addgene inc
Average 93 stars, based on 1 article reviews
rack1 - by Bioz Stars, 2026-04
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paav ef1α inteinc crec plasmids  (Addgene inc)
93
Addgene inc paav ef1α inteinc crec plasmids
Fig. 4 | AIM2 promotes Treg cells in vitro and restrains AKT–mTOR via the <t>RACK1–PP2A</t> complex. a, b, RT–PCR (a) and flow cytometry (b) of FOXP3 in wild-type and Aim2−/− CD4+ T cells activated with the indicated amounts (a) or 2 ng ml−1 (b) of TGFβ for 4 days; n = 5 experiments in a and n = 4 experiments in b. c, Flow cytometry of IFNγ+ or IL-17A+ CD4+ T cells of indicated genotypes, 4 days after differentiation under indicated polarizing conditions; n = 4 experiments. cTH17, classic TH17; pTH17, pathogenic TH17. d–g, Wild-type and Aim2−/− CD4+ T cells were stimulated as in b. d, Enrichment scores of indicated gene sets, based on RNA-seq datasets. e, f, ECAR (e) and OCR (f) levels during glycolysis, and OCR levels during fatty acid oxidation (g), by Seahorse analysis; n = 10 experiments for e; n = 5 experiments for f; and n = 3 experiments for g. h, Immunoblotting of indicated proteins in wild-type and Aim2−/− CD4+ T cells stimulated as in b with indicated treatment for 24 h. Representative of three
Paav Ef1α Inteinc Crec Plasmids, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti trem2 antibody novus biologicals  (Addgene inc)
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Addgene inc anti trem2 antibody novus biologicals
Fig. 4 | AIM2 promotes Treg cells in vitro and restrains AKT–mTOR via the <t>RACK1–PP2A</t> complex. a, b, RT–PCR (a) and flow cytometry (b) of FOXP3 in wild-type and Aim2−/− CD4+ T cells activated with the indicated amounts (a) or 2 ng ml−1 (b) of TGFβ for 4 days; n = 5 experiments in a and n = 4 experiments in b. c, Flow cytometry of IFNγ+ or IL-17A+ CD4+ T cells of indicated genotypes, 4 days after differentiation under indicated polarizing conditions; n = 4 experiments. cTH17, classic TH17; pTH17, pathogenic TH17. d–g, Wild-type and Aim2−/− CD4+ T cells were stimulated as in b. d, Enrichment scores of indicated gene sets, based on RNA-seq datasets. e, f, ECAR (e) and OCR (f) levels during glycolysis, and OCR levels during fatty acid oxidation (g), by Seahorse analysis; n = 10 experiments for e; n = 5 experiments for f; and n = 3 experiments for g. h, Immunoblotting of indicated proteins in wild-type and Aim2−/− CD4+ T cells stimulated as in b with indicated treatment for 24 h. Representative of three
Anti Trem2 Antibody Novus Biologicals, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dr phil sharp  (Addgene inc)
93
Addgene inc dr phil sharp
Fig. 4 | AIM2 promotes Treg cells in vitro and restrains AKT–mTOR via the <t>RACK1–PP2A</t> complex. a, b, RT–PCR (a) and flow cytometry (b) of FOXP3 in wild-type and Aim2−/− CD4+ T cells activated with the indicated amounts (a) or 2 ng ml−1 (b) of TGFβ for 4 days; n = 5 experiments in a and n = 4 experiments in b. c, Flow cytometry of IFNγ+ or IL-17A+ CD4+ T cells of indicated genotypes, 4 days after differentiation under indicated polarizing conditions; n = 4 experiments. cTH17, classic TH17; pTH17, pathogenic TH17. d–g, Wild-type and Aim2−/− CD4+ T cells were stimulated as in b. d, Enrichment scores of indicated gene sets, based on RNA-seq datasets. e, f, ECAR (e) and OCR (f) levels during glycolysis, and OCR levels during fatty acid oxidation (g), by Seahorse analysis; n = 10 experiments for e; n = 5 experiments for f; and n = 3 experiments for g. h, Immunoblotting of indicated proteins in wild-type and Aim2−/− CD4+ T cells stimulated as in b with indicated treatment for 24 h. Representative of three
Dr Phil Sharp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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plasmid constructs  (Addgene inc)
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Addgene inc plasmid constructs
Fig. 4 | AIM2 promotes Treg cells in vitro and restrains AKT–mTOR via the <t>RACK1–PP2A</t> complex. a, b, RT–PCR (a) and flow cytometry (b) of FOXP3 in wild-type and Aim2−/− CD4+ T cells activated with the indicated amounts (a) or 2 ng ml−1 (b) of TGFβ for 4 days; n = 5 experiments in a and n = 4 experiments in b. c, Flow cytometry of IFNγ+ or IL-17A+ CD4+ T cells of indicated genotypes, 4 days after differentiation under indicated polarizing conditions; n = 4 experiments. cTH17, classic TH17; pTH17, pathogenic TH17. d–g, Wild-type and Aim2−/− CD4+ T cells were stimulated as in b. d, Enrichment scores of indicated gene sets, based on RNA-seq datasets. e, f, ECAR (e) and OCR (f) levels during glycolysis, and OCR levels during fatty acid oxidation (g), by Seahorse analysis; n = 10 experiments for e; n = 5 experiments for f; and n = 3 experiments for g. h, Immunoblotting of indicated proteins in wild-type and Aim2−/− CD4+ T cells stimulated as in b with indicated treatment for 24 h. Representative of three
Plasmid Constructs, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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retroviral construct mscv  (Addgene inc)
94
Addgene inc retroviral construct mscv
KEY RESOURCES TABLE
Retroviral Construct Mscv, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mscv luciferase pgk hygro plasmid  (Addgene inc)
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Addgene inc mscv luciferase pgk hygro plasmid
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Mscv Luciferase Pgk Hygro Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dr scott lowe  (Addgene inc)
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Addgene inc dr scott lowe
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Dr Scott Lowe, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hpv16 e7  (Addgene inc)
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Hpv16 E7, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human nsd3 short cdna  (Addgene inc)
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Human Nsd3 Short Cdna, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Fig. 4 | AIM2 promotes Treg cells in vitro and restrains AKT–mTOR via the RACK1–PP2A complex. a, b, RT–PCR (a) and flow cytometry (b) of FOXP3 in wild-type and Aim2−/− CD4+ T cells activated with the indicated amounts (a) or 2 ng ml−1 (b) of TGFβ for 4 days; n = 5 experiments in a and n = 4 experiments in b. c, Flow cytometry of IFNγ+ or IL-17A+ CD4+ T cells of indicated genotypes, 4 days after differentiation under indicated polarizing conditions; n = 4 experiments. cTH17, classic TH17; pTH17, pathogenic TH17. d–g, Wild-type and Aim2−/− CD4+ T cells were stimulated as in b. d, Enrichment scores of indicated gene sets, based on RNA-seq datasets. e, f, ECAR (e) and OCR (f) levels during glycolysis, and OCR levels during fatty acid oxidation (g), by Seahorse analysis; n = 10 experiments for e; n = 5 experiments for f; and n = 3 experiments for g. h, Immunoblotting of indicated proteins in wild-type and Aim2−/− CD4+ T cells stimulated as in b with indicated treatment for 24 h. Representative of three

Journal: Nature

Article Title: AIM2 in regulatory T cells restrains autoimmune diseases.

doi: 10.1038/s41586-021-03231-w

Figure Lengend Snippet: Fig. 4 | AIM2 promotes Treg cells in vitro and restrains AKT–mTOR via the RACK1–PP2A complex. a, b, RT–PCR (a) and flow cytometry (b) of FOXP3 in wild-type and Aim2−/− CD4+ T cells activated with the indicated amounts (a) or 2 ng ml−1 (b) of TGFβ for 4 days; n = 5 experiments in a and n = 4 experiments in b. c, Flow cytometry of IFNγ+ or IL-17A+ CD4+ T cells of indicated genotypes, 4 days after differentiation under indicated polarizing conditions; n = 4 experiments. cTH17, classic TH17; pTH17, pathogenic TH17. d–g, Wild-type and Aim2−/− CD4+ T cells were stimulated as in b. d, Enrichment scores of indicated gene sets, based on RNA-seq datasets. e, f, ECAR (e) and OCR (f) levels during glycolysis, and OCR levels during fatty acid oxidation (g), by Seahorse analysis; n = 10 experiments for e; n = 5 experiments for f; and n = 3 experiments for g. h, Immunoblotting of indicated proteins in wild-type and Aim2−/− CD4+ T cells stimulated as in b with indicated treatment for 24 h. Representative of three

Article Snippet: Ectopic expression of PP2A and RACK1 in Treg cells To generate the retrovirus expressing PP2A and RACK1, we first cloned PP2A (OriGene Technologies, MR204384L4) and RACK1 (OriGene Technologies, MR204575L3) into retroviral vectors MSCV-IRES-Thy1.1 (MIT, Addgene 17442) and MSCV-IRES-GFP (MIG, Addgene 20672) respectively, and generated MIT-PP2A and MIG-RACK1 retrovirus in 293T cells by transient transfection.

Techniques: In Vitro, Reverse Transcription Polymerase Chain Reaction, Flow Cytometry, RNA Sequencing, Western Blot

KEY RESOURCES TABLE

Journal: Cell reports

Article Title: A PRMT5-RNF168-SMURF2 Axis Controls H2AX Proteostasis

doi: 10.1016/j.celrep.2019.08.031

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Cell lines with exogenous overexpression of H2AX, PRMT5, RNF168 and SMURF2 were obtained using retroviral construct MSCV (Addgene, plasmid #24828).

Techniques: Ubiquitin Proteomics, Virus, Recombinant, Cell Culture, Stem Cell Culture, Protease Inhibitor, Mutagenesis, CCK-8 Assay, RNA Extraction, SYBR Green Assay, Plasmid Preparation, shRNA, Software, Microscopy, Membrane