ms2 aptamers Search Results


genes for reca-2a-mcherry and sgrna containing ms2-binding aptamers (sgrna-ms2)  (GenScript corporation)
90
GenScript corporation genes for reca-2a-mcherry and sgrna containing ms2-binding aptamers (sgrna-ms2)
( A ) Schematic of Cas9- or Cas9/RecA-mediated HDR gene editing. Cas9-mediated HDR required sgRNA, spCas9, and ssDNA donors (dot line frame). Cas9/RecA-mediated HDR required sgRNA, spCas9, RecA, and ssDNA donors (solid line frame). RecA was enriched near sgRNA via the <t>MS2-coated</t> protein. ( B ) Experimental scheme for gene editing by NHEJ or HDR in the BFP HEK293FT line. ( C ) Representative FACS analysis of HEK293FT cells from the control (Cas9 without sgRNA group). ( D ) Representative FACS analysis of HEK293FT cells treated in the Cas9 group, with Cas9, sgRNA, and ssDNA donor. ( E ) Representative FACS analysis of HEK293FT cells treated in the Cas9/RecA group, with Cas9, sgRNA, ssDNA donor, and RecA. ( F ) Quantification results of Cas9-mediated HDR and NHEJ efficiencies and Cas9/RecA-mediated HDR and NHEJ efficiencies. n = 7. * P < 0.05 and ** P < 0.01, paired Student’s t test. n.s., no significance.
Genes For Reca 2a Mcherry And Sgrna Containing Ms2 Binding Aptamers (Sgrna Ms2), supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/genes for reca-2a-mcherry and sgrna containing ms2-binding aptamers (sgrna-ms2)/product/GenScript corporation
Average 90 stars, based on 1 article reviews
genes for reca-2a-mcherry and sgrna containing ms2-binding aptamers (sgrna-ms2) - by Bioz Stars, 2026-06
90/100 stars
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( A ) Schematic of Cas9- or Cas9/RecA-mediated HDR gene editing. Cas9-mediated HDR required sgRNA, spCas9, and ssDNA donors (dot line frame). Cas9/RecA-mediated HDR required sgRNA, spCas9, RecA, and ssDNA donors (solid line frame). RecA was enriched near sgRNA via the MS2-coated protein. ( B ) Experimental scheme for gene editing by NHEJ or HDR in the BFP HEK293FT line. ( C ) Representative FACS analysis of HEK293FT cells from the control (Cas9 without sgRNA group). ( D ) Representative FACS analysis of HEK293FT cells treated in the Cas9 group, with Cas9, sgRNA, and ssDNA donor. ( E ) Representative FACS analysis of HEK293FT cells treated in the Cas9/RecA group, with Cas9, sgRNA, ssDNA donor, and RecA. ( F ) Quantification results of Cas9-mediated HDR and NHEJ efficiencies and Cas9/RecA-mediated HDR and NHEJ efficiencies. n = 7. * P < 0.05 and ** P < 0.01, paired Student’s t test. n.s., no significance.

Journal: Science Advances

Article Title: In vivo genome editing rescues photoreceptor degeneration via a Cas9/RecA-mediated homology-directed repair pathway

doi: 10.1126/sciadv.aav3335

Figure Lengend Snippet: ( A ) Schematic of Cas9- or Cas9/RecA-mediated HDR gene editing. Cas9-mediated HDR required sgRNA, spCas9, and ssDNA donors (dot line frame). Cas9/RecA-mediated HDR required sgRNA, spCas9, RecA, and ssDNA donors (solid line frame). RecA was enriched near sgRNA via the MS2-coated protein. ( B ) Experimental scheme for gene editing by NHEJ or HDR in the BFP HEK293FT line. ( C ) Representative FACS analysis of HEK293FT cells from the control (Cas9 without sgRNA group). ( D ) Representative FACS analysis of HEK293FT cells treated in the Cas9 group, with Cas9, sgRNA, and ssDNA donor. ( E ) Representative FACS analysis of HEK293FT cells treated in the Cas9/RecA group, with Cas9, sgRNA, ssDNA donor, and RecA. ( F ) Quantification results of Cas9-mediated HDR and NHEJ efficiencies and Cas9/RecA-mediated HDR and NHEJ efficiencies. n = 7. * P < 0.05 and ** P < 0.01, paired Student’s t test. n.s., no significance.

Article Snippet: Genes for RecA-2a-mCherry and sgRNA containing MS2-binding aptamers (sgRNA-ms2) were synthesized in GenScript.

Techniques: Control