Journal: Korean Journal for Food Science of Animal Resources

Article Title: Lactobacillus plantarum DR7 Reduces Cholesterol via Phosphorylation of AMPK That Down-regulated the mRNA Expression of HMG-CoA Reductase

doi: 10.5851/kosfa.2018.38.2.350

Figure Lengend Snippet: Serum-free Dulbecco's modified Eagle's medium (DMEM) used contained 1 mM of cholesterol and 0.03% of ox-bile. Results are expressed as mean; error bars (SEM); n=3. Statistical analysis was performed using one-way ANOVA with Duncan’s post hoc comparisons. Means labelled with different letters are significantly different ( p <0.05). Blank MRS: sterile de Mann, Rogosa, Sharpe (MRS) broth at a final concentration of 50% (v/v) in serum-free DMEM; 30242, Lactobacillus reuteri NCIMB30242; DR9, Lactobacillus fermentum DR9; DR7, Lactobacillus plantarum DR7; 8513d, Lactobacillus reuteri 8513d, Shirota: Lactobacillus casei Shirota.

Article Snippet: All stock cultures were preserved in 20% glycerol (-20°C), activated in sterile de Mann, Rogosa, Sharpe (MRS) broth (Hi-media, Mumbai, India) for three successive times using 10% (v/v) inoculums and incubated at 37°C for 24 h prior to use.

Techniques: Modification, Sterility, Concentration Assay

Journal: Korean Journal for Food Science of Animal Resources

Article Title: Lactobacillus plantarum DR7 Reduces Cholesterol via Phosphorylation of AMPK That Down-regulated the mRNA Expression of HMG-CoA Reductase

doi: 10.5851/kosfa.2018.38.2.350

Figure Lengend Snippet: Results are expressed as mean; error bars (SEM); n=3. Statistical analysis was performed using one-way ANOVA with Duncan’s post hoc comparisons. Means labelled with different letters are significantly different ( p <0.05). Non-treated: HepG2 cultured in serum-free Dulbecco's modified Eagle's medium (DMEM) without any treatment; blank MRS: HepG2 cultured in sterile de Mann, Rogosa, Sharpe (MRS) broth at a final concentration of 50% (v/v) in serum-free DMEM.

Article Snippet: All stock cultures were preserved in 20% glycerol (-20°C), activated in sterile de Mann, Rogosa, Sharpe (MRS) broth (Hi-media, Mumbai, India) for three successive times using 10% (v/v) inoculums and incubated at 37°C for 24 h prior to use.

Techniques: Cell Culture, Modification, Sterility, Concentration Assay

Journal: Korean Journal for Food Science of Animal Resources

Article Title: Lactobacillus plantarum DR7 Reduces Cholesterol via Phosphorylation of AMPK That Down-regulated the mRNA Expression of HMG-CoA Reductase

doi: 10.5851/kosfa.2018.38.2.350

Figure Lengend Snippet: Results are expressed as mean; error bars (SEM); n=3. Statistical analysis was performed using one-way ANOVA with Duncan’s post hoc comparisons. Means labelled with different letters are significantly different ( p <0.05). AICAR (AMPK activator) and Compound C (AMPK inhibitor) were used as positive and negative control, respectively. Non-treated: HepG2 cultured in serum-free Dulbecco's modified Eagle's medium (DMEM) without any treatment; blank MRS: HepG2 cultured in sterile de Mann, Rogosa, Sharpe (MRS) broth at a final concentration of 30% (v/v) in serum-free DMEM; CFS-DR7, HepG2 cultured in CFS from L. plantarum DR7 at a final concentration of 30% (v/v) in serum-free DMEM.

Article Snippet: All stock cultures were preserved in 20% glycerol (-20°C), activated in sterile de Mann, Rogosa, Sharpe (MRS) broth (Hi-media, Mumbai, India) for three successive times using 10% (v/v) inoculums and incubated at 37°C for 24 h prior to use.

Techniques: Negative Control, Cell Culture, Modification, Sterility, Concentration Assay

Journal: Korean Journal for Food Science of Animal Resources

Article Title: Lactobacillus plantarum DR7 Reduces Cholesterol via Phosphorylation of AMPK That Down-regulated the mRNA Expression of HMG-CoA Reductase

doi: 10.5851/kosfa.2018.38.2.350

Figure Lengend Snippet: Results are expressed as mean; error bars (SEM); n=3. Statistical analysis was performed using one-way ANOVA with Duncan’s post hoc comparisons. Means labelled with different letters are significantly different ( p <0.05). AICAR (AMPK activator) and Compound C (AMPK inhibitor) were used as positive and negative control, respectively. Non-treated; HepG2 cultured in serum-free Dulbecco's modified Eagle's medium (DMEM) without any treatment; blank MRS: HepG2 cultured in sterile de Mann, Rogosa, Sharpe (MRS) broth at a final concentration of 30% (v/v) in serum-free DMEM; CFS-DR7: HepG2 cultured in CFS from L. plantarum DR7 at a final concentration of 30% (v/v) in serum-free DMEM.

Article Snippet: All stock cultures were preserved in 20% glycerol (-20°C), activated in sterile de Mann, Rogosa, Sharpe (MRS) broth (Hi-media, Mumbai, India) for three successive times using 10% (v/v) inoculums and incubated at 37°C for 24 h prior to use.

Techniques: Negative Control, Cell Culture, Modification, Sterility, Concentration Assay

Journal: Korean Journal for Food Science of Animal Resources

Article Title: Lactobacillus plantarum DR7 Reduces Cholesterol via Phosphorylation of AMPK That Down-regulated the mRNA Expression of HMG-CoA Reductase

doi: 10.5851/kosfa.2018.38.2.350

Figure Lengend Snippet: Results are expressed as mean; error bars (SEM); n=3. Statistical analysis was performed using one-way ANOVA with Duncan’s post hoc comparisons. Means labelled with different letters are significantly different ( p <0.05). AICAR (AMPK activator) and Compound C (AMPK inhibitor) were used as positive and negative control, respectively. Non-treated: HepG2 cultured in serum-free Dulbecco's modified Eagle's medium (DMEM) without any treatment; blank MRS, HepG2 cultured in sterile de Mann, Rogosa, Sharpe (MRS) broth at a final concentration of 30% (v/v) in serum-free DMEM; CFS-DR7, HepG2 cultured in CFS from Lactobacillus plantarum DR7 at a final concentration of 30% (v/v) in serum-free DMEM.

Article Snippet: All stock cultures were preserved in 20% glycerol (-20°C), activated in sterile de Mann, Rogosa, Sharpe (MRS) broth (Hi-media, Mumbai, India) for three successive times using 10% (v/v) inoculums and incubated at 37°C for 24 h prior to use.

Techniques: Negative Control, Cell Culture, Modification, Sterility, Concentration Assay