mrna assay Search Results


nebnext poly a mrna magnetic isolation module  (New England Biolabs)
99
New England Biolabs nebnext poly a mrna magnetic isolation module
Nebnext Poly A Mrna Magnetic Isolation Module, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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magnospheretm ultrapure mrna purification kit  (TaKaRa)
94
TaKaRa magnospheretm ultrapure mrna purification kit
Magnospheretm Ultrapure Mrna Purification Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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2 o methyltransferase  (New England Biolabs)
96
New England Biolabs 2 o methyltransferase
2 O Methyltransferase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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smart seq mrna kit  (TaKaRa)
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TaKaRa smart seq mrna kit
Smart Seq Mrna Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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igf2bp1  (Proteintech)
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Proteintech igf2bp1
Igf2bp1, supplied by Proteintech, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cpsf7  (Novus Biologicals)
90
Novus Biologicals cpsf7
Novel complexes copurifying with ICP4 (spectral counts).
Cpsf7, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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turbocapture 96 mrna kit  (Qiagen)
95
Qiagen turbocapture 96 mrna kit
Novel complexes copurifying with ICP4 (spectral counts).
Turbocapture 96 Mrna Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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truseq stranded mrna sample prep kit  (Illumina Inc)
99
Illumina Inc truseq stranded mrna sample prep kit
ACSL3 loss confers resistance to palmitic acid–induced cell death. (A) Left, ACSL3 protein by western blot; right, viability of ACSL3-KO and NC HLF cells after 72 hours PA. (B) Left, INSIG1 protein by western blot; right, viability of INSIG1-KO and NC HLF cells after 72 hours PA. (C, D) Primary human hepatocytes (isolated from humanized mice) transfected with ACSL3 siRNA; ACSL3 <t>mRNA</t> by RT-qPCR normalized to ACTB (C), and PA-induced changes in viability and caspase-3/7 activity (D). (E) Real-time imaging (IncuCyte) of confluence in ACSL3-KO and NC HLF cells during day 1–6 PA exposure. (F) Viability of HLF cells treated with PA with or without the pan-ACSL inhibitor triacsin. (G) Flow-cytometric quantification of Annexin V and propidium iodide-positive cells after 24 hours PA in ACSL3-KO versus NC HLF cells. (H) ACSL3 and GRP78 mRNA by RT-qPCR normalized to ACTB following PA exposure. Abbreviations: ACSL3, acyl-CoA synthetase long-chain family member 3; KO, knockout; NC, negative control; PA, palmitic acid; siRNA, small interfering RNA; WT, wild type.
Truseq Stranded Mrna Sample Prep Kit, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tianseq mrna  (tiangen biotech co)
93
tiangen biotech co tianseq mrna
ACSL3 loss confers resistance to palmitic acid–induced cell death. (A) Left, ACSL3 protein by western blot; right, viability of ACSL3-KO and NC HLF cells after 72 hours PA. (B) Left, INSIG1 protein by western blot; right, viability of INSIG1-KO and NC HLF cells after 72 hours PA. (C, D) Primary human hepatocytes (isolated from humanized mice) transfected with ACSL3 siRNA; ACSL3 <t>mRNA</t> by RT-qPCR normalized to ACTB (C), and PA-induced changes in viability and caspase-3/7 activity (D). (E) Real-time imaging (IncuCyte) of confluence in ACSL3-KO and NC HLF cells during day 1–6 PA exposure. (F) Viability of HLF cells treated with PA with or without the pan-ACSL inhibitor triacsin. (G) Flow-cytometric quantification of Annexin V and propidium iodide-positive cells after 24 hours PA in ACSL3-KO versus NC HLF cells. (H) ACSL3 and GRP78 mRNA by RT-qPCR normalized to ACTB following PA exposure. Abbreviations: ACSL3, acyl-CoA synthetase long-chain family member 3; KO, knockout; NC, negative control; PA, palmitic acid; siRNA, small interfering RNA; WT, wild type.
Tianseq Mrna, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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reafinitytm miltenyi biotec  (Miltenyi Biotec)
93
Miltenyi Biotec reafinitytm miltenyi biotec
ACSL3 loss confers resistance to palmitic acid–induced cell death. (A) Left, ACSL3 protein by western blot; right, viability of ACSL3-KO and NC HLF cells after 72 hours PA. (B) Left, INSIG1 protein by western blot; right, viability of INSIG1-KO and NC HLF cells after 72 hours PA. (C, D) Primary human hepatocytes (isolated from humanized mice) transfected with ACSL3 siRNA; ACSL3 <t>mRNA</t> by RT-qPCR normalized to ACTB (C), and PA-induced changes in viability and caspase-3/7 activity (D). (E) Real-time imaging (IncuCyte) of confluence in ACSL3-KO and NC HLF cells during day 1–6 PA exposure. (F) Viability of HLF cells treated with PA with or without the pan-ACSL inhibitor triacsin. (G) Flow-cytometric quantification of Annexin V and propidium iodide-positive cells after 24 hours PA in ACSL3-KO versus NC HLF cells. (H) ACSL3 and GRP78 mRNA by RT-qPCR normalized to ACTB following PA exposure. Abbreviations: ACSL3, acyl-CoA synthetase long-chain family member 3; KO, knockout; NC, negative control; PA, palmitic acid; siRNA, small interfering RNA; WT, wild type.
Reafinitytm Miltenyi Biotec, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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flp recombinase mrna  (Miltenyi Biotec)
93
Miltenyi Biotec flp recombinase mrna
ACSL3 loss confers resistance to palmitic acid–induced cell death. (A) Left, ACSL3 protein by western blot; right, viability of ACSL3-KO and NC HLF cells after 72 hours PA. (B) Left, INSIG1 protein by western blot; right, viability of INSIG1-KO and NC HLF cells after 72 hours PA. (C, D) Primary human hepatocytes (isolated from humanized mice) transfected with ACSL3 siRNA; ACSL3 <t>mRNA</t> by RT-qPCR normalized to ACTB (C), and PA-induced changes in viability and caspase-3/7 activity (D). (E) Real-time imaging (IncuCyte) of confluence in ACSL3-KO and NC HLF cells during day 1–6 PA exposure. (F) Viability of HLF cells treated with PA with or without the pan-ACSL inhibitor triacsin. (G) Flow-cytometric quantification of Annexin V and propidium iodide-positive cells after 24 hours PA in ACSL3-KO versus NC HLF cells. (H) ACSL3 and GRP78 mRNA by RT-qPCR normalized to ACTB following PA exposure. Abbreviations: ACSL3, acyl-CoA synthetase long-chain family member 3; KO, knockout; NC, negative control; PA, palmitic acid; siRNA, small interfering RNA; WT, wild type.
Flp Recombinase Mrna, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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multimacs mrna isolation kit  (Miltenyi Biotec)
94
Miltenyi Biotec multimacs mrna isolation kit
ACSL3 loss confers resistance to palmitic acid–induced cell death. (A) Left, ACSL3 protein by western blot; right, viability of ACSL3-KO and NC HLF cells after 72 hours PA. (B) Left, INSIG1 protein by western blot; right, viability of INSIG1-KO and NC HLF cells after 72 hours PA. (C, D) Primary human hepatocytes (isolated from humanized mice) transfected with ACSL3 siRNA; ACSL3 <t>mRNA</t> by RT-qPCR normalized to ACTB (C), and PA-induced changes in viability and caspase-3/7 activity (D). (E) Real-time imaging (IncuCyte) of confluence in ACSL3-KO and NC HLF cells during day 1–6 PA exposure. (F) Viability of HLF cells treated with PA with or without the pan-ACSL inhibitor triacsin. (G) Flow-cytometric quantification of Annexin V and propidium iodide-positive cells after 24 hours PA in ACSL3-KO versus NC HLF cells. (H) ACSL3 and GRP78 mRNA by RT-qPCR normalized to ACTB following PA exposure. Abbreviations: ACSL3, acyl-CoA synthetase long-chain family member 3; KO, knockout; NC, negative control; PA, palmitic acid; siRNA, small interfering RNA; WT, wild type.
Multimacs Mrna Isolation Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Novel complexes copurifying with ICP4 (spectral counts).

Journal: PLoS ONE

Article Title: Temporal Association of Herpes Simplex Virus ICP4 with Cellular Complexes Functioning at Multiple Steps in PolII Transcription

doi: 10.1371/journal.pone.0078242

Figure Lengend Snippet: Novel complexes copurifying with ICP4 (spectral counts).

Article Snippet: The antibodies used to probe the membranes were; N15, polyclonal rabbit serum, for ICP4 (1:500), Trap220/Med 1 (sc-8998-X), Med6 (sc-9434), Med 4 (NBP1-84977; Novus Biologicals), Med23 (550429; BD Bio), CDK8 (sc-1521), Med12 (NB100-2357; Novus Biologicals), Med 13 ((NB100-60642; Novus Biologicals), Med 26 (sc-166614), TAF1 (sc735), TBP (233-R Covance), p62 (sc292-X), p80/XPB (sc-20696-x), CPSF2 (sc-165983), CPSF7 (NB100-61600; Novus Biologicals), Brahma (sc-6450), CHD3 (ABE86; Milipore), RUVBL1 (06-1299; Milipore), RUVBL2 (06-1300; Milipore) at the manufacturers recommended dilutions.

Techniques:

Western blot analysis of nuclear extracts (NE) or affinity purified (PD) samples collected at 6 hpi using either wtICP4 (KOS) or TAP-wtICP4 (TAP) expressing viruses. Antibodies directed against (A) p80 and p62 of TFIIH, (B) CPSF2 and CPSF7 of the cleavage and polyadenylation complex and (C) Brahma of Swi/Snf, CHD3 of the Nurd complex, and RuvBL1 and RuvBL2 of the Ino80 complex were used.

Journal: PLoS ONE

Article Title: Temporal Association of Herpes Simplex Virus ICP4 with Cellular Complexes Functioning at Multiple Steps in PolII Transcription

doi: 10.1371/journal.pone.0078242

Figure Lengend Snippet: Western blot analysis of nuclear extracts (NE) or affinity purified (PD) samples collected at 6 hpi using either wtICP4 (KOS) or TAP-wtICP4 (TAP) expressing viruses. Antibodies directed against (A) p80 and p62 of TFIIH, (B) CPSF2 and CPSF7 of the cleavage and polyadenylation complex and (C) Brahma of Swi/Snf, CHD3 of the Nurd complex, and RuvBL1 and RuvBL2 of the Ino80 complex were used.

Article Snippet: The antibodies used to probe the membranes were; N15, polyclonal rabbit serum, for ICP4 (1:500), Trap220/Med 1 (sc-8998-X), Med6 (sc-9434), Med 4 (NBP1-84977; Novus Biologicals), Med23 (550429; BD Bio), CDK8 (sc-1521), Med12 (NB100-2357; Novus Biologicals), Med 13 ((NB100-60642; Novus Biologicals), Med 26 (sc-166614), TAF1 (sc735), TBP (233-R Covance), p62 (sc292-X), p80/XPB (sc-20696-x), CPSF2 (sc-165983), CPSF7 (NB100-61600; Novus Biologicals), Brahma (sc-6450), CHD3 (ABE86; Milipore), RUVBL1 (06-1299; Milipore), RUVBL2 (06-1300; Milipore) at the manufacturers recommended dilutions.

Techniques: Western Blot, Affinity Purification, Expressing

ACSL3 loss confers resistance to palmitic acid–induced cell death. (A) Left, ACSL3 protein by western blot; right, viability of ACSL3-KO and NC HLF cells after 72 hours PA. (B) Left, INSIG1 protein by western blot; right, viability of INSIG1-KO and NC HLF cells after 72 hours PA. (C, D) Primary human hepatocytes (isolated from humanized mice) transfected with ACSL3 siRNA; ACSL3 mRNA by RT-qPCR normalized to ACTB (C), and PA-induced changes in viability and caspase-3/7 activity (D). (E) Real-time imaging (IncuCyte) of confluence in ACSL3-KO and NC HLF cells during day 1–6 PA exposure. (F) Viability of HLF cells treated with PA with or without the pan-ACSL inhibitor triacsin. (G) Flow-cytometric quantification of Annexin V and propidium iodide-positive cells after 24 hours PA in ACSL3-KO versus NC HLF cells. (H) ACSL3 and GRP78 mRNA by RT-qPCR normalized to ACTB following PA exposure. Abbreviations: ACSL3, acyl-CoA synthetase long-chain family member 3; KO, knockout; NC, negative control; PA, palmitic acid; siRNA, small interfering RNA; WT, wild type.

Journal: Hepatology Communications

Article Title: Genome-wide CRISPR screen identifies ACSL3 as a regulator of lipotoxicity and progression of MASLD

doi: 10.1097/HC9.0000000000000884

Figure Lengend Snippet: ACSL3 loss confers resistance to palmitic acid–induced cell death. (A) Left, ACSL3 protein by western blot; right, viability of ACSL3-KO and NC HLF cells after 72 hours PA. (B) Left, INSIG1 protein by western blot; right, viability of INSIG1-KO and NC HLF cells after 72 hours PA. (C, D) Primary human hepatocytes (isolated from humanized mice) transfected with ACSL3 siRNA; ACSL3 mRNA by RT-qPCR normalized to ACTB (C), and PA-induced changes in viability and caspase-3/7 activity (D). (E) Real-time imaging (IncuCyte) of confluence in ACSL3-KO and NC HLF cells during day 1–6 PA exposure. (F) Viability of HLF cells treated with PA with or without the pan-ACSL inhibitor triacsin. (G) Flow-cytometric quantification of Annexin V and propidium iodide-positive cells after 24 hours PA in ACSL3-KO versus NC HLF cells. (H) ACSL3 and GRP78 mRNA by RT-qPCR normalized to ACTB following PA exposure. Abbreviations: ACSL3, acyl-CoA synthetase long-chain family member 3; KO, knockout; NC, negative control; PA, palmitic acid; siRNA, small interfering RNA; WT, wild type.

Article Snippet: Library preparation was performed in the Genomics Core (Institute of Microbiology) using the TruSeq Stranded mRNA Sample Prep Kit (Illumina) on an Apollo Library Prep System (Takara).

Techniques: Western Blot, Isolation, Transfection, Quantitative RT-PCR, Activity Assay, Imaging, Knock-Out, Negative Control, Small Interfering RNA

ACSL3 deficiency abrogates PA-induced activation of lipogenic programs without enhancing β-oxidation. (A–C) mRNA levels by RT-qPCR normalized to ACTB: CPT1, CPT2, and ACOX (A); SCD, ChREBP, and SREBP1 (B); PPARG and LXRA (C). Abbreviations: KO, knockout; WT, wild type.

Journal: Hepatology Communications

Article Title: Genome-wide CRISPR screen identifies ACSL3 as a regulator of lipotoxicity and progression of MASLD

doi: 10.1097/HC9.0000000000000884

Figure Lengend Snippet: ACSL3 deficiency abrogates PA-induced activation of lipogenic programs without enhancing β-oxidation. (A–C) mRNA levels by RT-qPCR normalized to ACTB: CPT1, CPT2, and ACOX (A); SCD, ChREBP, and SREBP1 (B); PPARG and LXRA (C). Abbreviations: KO, knockout; WT, wild type.

Article Snippet: Library preparation was performed in the Genomics Core (Institute of Microbiology) using the TruSeq Stranded mRNA Sample Prep Kit (Illumina) on an Apollo Library Prep System (Takara).

Techniques: Activation Assay, Quantitative RT-PCR, Knock-Out