Journal: npj Viruses

Article Title: SARS-CoV-2 crossreactive B-cells outnumber seasonal coronavirus spike-specific clones at the end of the COVID-19 pandemic

doi: 10.1038/s44298-026-00185-6

Figure Lengend Snippet: a Serum IgG and b IgA 50% endpoint antibody titers towards prefusion stabilized spike (S-trimer), the S1 and S2 subdomains of spike and the receptor binding domain (RBD) of the ancestral SARS-CoV-2 strain (Anc.) and delta, omicron BA.1 and BA.5 variants and the seasonal human coronaviruses (sHCoVs) 229E, NL63, HKU1 and OC43 were determined using protein microarray from healthy individuals sampled before (blue) or at the end of the pandemic (red). Mean titers are indicated on top and the number of seroconverted individuals is indicated below the graph. The lower limit of detection (LLoD) is indicated by a dotted line. c The frequency of reactive IgG and d IgA B-cells was determined by B-cell profiling for 8 donors sampled pre- (blue) and 8 donors sampled end-pandemic (red). Center line, median; boxes, upper and lower quartiles; significance of titer differences and B-cell frequencies for sHCoVs were calculated using a Mann-Whitney test and shown as * p < 0.05 or ** p < 0.01.

Article Snippet: The OC43 strain VR-1558 (ATCC, CCL-244) was propagated in MRC-5 cells (ATCC, CCL-171).

Techniques: Binding Assay, Microarray, MANN-WHITNEY

Journal: npj Viruses

Article Title: SARS-CoV-2 crossreactive B-cells outnumber seasonal coronavirus spike-specific clones at the end of the COVID-19 pandemic

doi: 10.1038/s44298-026-00185-6

Figure Lengend Snippet: a Type specific and cross-reactive clones targeting SARS-CoV-2 and alpha-CoV or SARS-CoV-2 and beta-CoV pre- and end-pandemic are enumerated and plotted in Venn diagrams for S-trimer, S1, and S2. b The fraction of clones that are SARS-CoV-2-specific (red), sHCoV-specific (blue), or cross-reactive (green) from pooled pre- and pooled end-pandemic donors is plotted. c Proportion of beta-CoV clones, including SARS-CoV-2, that are reactive towards S1 (orange) or S2 (dark red) pre- and end-pandemic. d The level of reactivity of SARS-CoV-2, 229E and NL63 S1-reactive (left panel) as well as SARS-CoV-2, HKU1 and OC43 S2-reactive IgG clones (right panel) detected in end-pandemic donors is indicated as the mean fluorescent intensity (MFI; range: 1,000-65,350). Cross-reactivity of clones is indicated by a connecting line.

Article Snippet: The OC43 strain VR-1558 (ATCC, CCL-244) was propagated in MRC-5 cells (ATCC, CCL-171).

Techniques: Clone Assay

Journal: npj Viruses

Article Title: SARS-CoV-2 crossreactive B-cells outnumber seasonal coronavirus spike-specific clones at the end of the COVID-19 pandemic

doi: 10.1038/s44298-026-00185-6

Figure Lengend Snippet: a Serum OC43 neutralization potential was determined using 50% focus reduction neutralization tests (FRNT50) and compared between pre- (blue) and end-pandemic (red) donors. Statistical significance was determined by Mann–Whitney U -test. b Linear regression analysis between serum OC43 neutralization titers and OC43 IgG binding titers (pre-pandemic, blue) or OC43 and SARS-CoV-2 IgG binding titers (end-pandemic, red). The regression line and 95% confidence interval are plotted for significant correlations ( p < 0.05). c The OC43 neutralization titers of sera depleted of OC43 S1-specific antibodies (left panel) (Wilcoxon paired t -test), and sera depleted for OC43 S2- or SARS-CoV-2 S2-specific antibodies (right panel), normalized to mock-depleted sera are shown. Statistical significance was determined between the original titers by one-way ANOVA with Geisser-Greenhouse correction. d IgG B-cell culture supernatants that were OC43 S1-specific, S2-specific, or SARS-CoV-2/OC43 S2-cross-reactive were tested in a OC43 focus reduction assay and compared with non-reactive controls (unpaired Student’s t-test). Significant differences were plotted as * p < 0.05, ** p < 0.01 and *** p < 0.001.

Article Snippet: The OC43 strain VR-1558 (ATCC, CCL-244) was propagated in MRC-5 cells (ATCC, CCL-171).

Techniques: Neutralization, MANN-WHITNEY, Binding Assay, Cell Culture

Journal: Frontiers in Microbiology

Article Title: Transcription factors Asg1p and Hal9p regulate pH homeostasis in Candida glabrata

doi: 10.3389/fmicb.2015.00843

Figure Lengend Snippet: Strains and plasmids used in this study .

Article Snippet: C. glabrata ATCC 55/ GFP , C. glabrata ATCC 55 (pY13- GFP ) , This study.

Techniques: Mutagenesis

Journal: Frontiers in Microbiology

Article Title: Transcription factors Asg1p and Hal9p regulate pH homeostasis in Candida glabrata

doi: 10.3389/fmicb.2015.00843

Figure Lengend Snippet: Growth assays in different YNB media. (A) Deletion of CgASG1 has no effect on the utilization of non-fermentative carbon sources in C. glabrata . (B) CgAsg1p plays a role under acid-stress conditions. (C) CgHAL9 serves an important role in cell growth under hypersaline conditions in C. glabrata . (D) CgHal9p plays a role under acid-stress conditions. Logarithmic-phase cells of each C. glabrata strain were adjusted to 2 × 10 7 cells/mL, and then 4 μL of serial tenfold dilutions were spotted onto the corresponding YNB media, as indicated. Pictures were taken after 4 days of growth at 30°C.

Article Snippet: C. glabrata ATCC 55/ GFP , C. glabrata ATCC 55 (pY13- GFP ) , This study.

Techniques:

Journal: Frontiers in Microbiology

Article Title: Transcription factors Asg1p and Hal9p regulate pH homeostasis in Candida glabrata

doi: 10.3389/fmicb.2015.00843

Figure Lengend Snippet: Intracellular pH of wild-type, mutant and complemented strains of C. glabrata as below ^a .

Article Snippet: C. glabrata ATCC 55/ GFP , C. glabrata ATCC 55 (pY13- GFP ) , This study.

Techniques: Mutagenesis

Journal: Frontiers in Microbiology

Article Title: Transcription factors Asg1p and Hal9p regulate pH homeostasis in Candida glabrata

doi: 10.3389/fmicb.2015.00843

Figure Lengend Snippet: Quantitative reverse-transcribed PCR (qRT-PCR) validation of transcriptional profiles in an acidic environment . Logarithmic-phase C. glabrata cells were incubated in YNB or YNB-pH 2.0 media for 2 h. qRT-PCR analyses of the indicated genes was performed as described in the Materials and Methods Section. The means and standard deviations for three independent experiments are shown. C. glabrata strains: wild-type, Cgasg1 Δ and Cghal9 Δ strains. ( * P < 0.05 compared to the corresponding wild-type control, as determined by t -test).

Article Snippet: C. glabrata ATCC 55/ GFP , C. glabrata ATCC 55 (pY13- GFP ) , This study.

Techniques: Reverse Transcription, Quantitative RT-PCR, Biomarker Discovery, Incubation, Control

Journal: Stroke

Article Title: Neuronal CD200 Signaling Is Protective in the Acute Phase of Ischemic Stroke.

doi: 10.1161/STROKEAHA.120.032374

Figure Lengend Snippet: Figure 1. Effects of neuronal CD200 signaling on stroke outcomes and neuronal apoptosis at 3 days after stroke. (A) Representative images of brain slices stained with cresyl violet (CV) and quantification of brain infarct volumes, NDS, and corner test scores in TMX vs. VEH mice. (B) TUNEL labelled cell apoptosis in peri-infarct area of TMX and VEH treated mice and the quantification of percent of TUNEL positive cells in ipsilateral hemispheres Each dot represents the average of % apoptotic cells from eight 20× fields/animal in the peri-infarct area. N=3 for sham and 6–7 for stroke per group; *P < 0.05.

Article Snippet: Determination of CD200 and cytokine Levels The levels of CD200 in mouse brain, plasma and in the supernatants of primary neuronal culture were measured by a sandwich ELISA kit according to manufacture instruction ((# EK1185, Mouse CD200 PicoKine ELISA Kit, Boster Biological, Pleasanton, CA, USA.).

Techniques: Staining, TUNEL Assay

Journal: Stroke

Article Title: Neuronal CD200 Signaling Is Protective in the Acute Phase of Ischemic Stroke.

doi: 10.1161/STROKEAHA.120.032374

Figure Lengend Snippet: Figure 4. Plasma cytokine levels after ischemic injury. Pro-inflammatory (TNFα, IL-1β and IL-6) and anti-inflammatory (IL-10 and IL-4) cytokine levels (pg/mL) were measured in the plasma of VEH and TMX treated sham/stroke mice at 3d (A) and 7d (B). N=5–6 sham and 6–7 stroke animals per group; *P < 0.05. (C) CD200 levels in naïve brains (left), in the plasma (middle) of TMX and VEH treated mice, and in neuronal culture medium after OGD (right). N=5–6 sham and 6–8 stroke animals per group; *P < 0.05. For neuronal culture medium, N=4 independent OGD experiments; *P < 0.05.

Article Snippet: Determination of CD200 and cytokine Levels The levels of CD200 in mouse brain, plasma and in the supernatants of primary neuronal culture were measured by a sandwich ELISA kit according to manufacture instruction ((# EK1185, Mouse CD200 PicoKine ELISA Kit, Boster Biological, Pleasanton, CA, USA.).

Techniques: Clinical Proteomics

Journal: Stroke

Article Title: Neuronal CD200 Signaling Is Protective in the Acute Phase of Ischemic Stroke.

doi: 10.1161/STROKEAHA.120.032374

Figure Lengend Snippet: Figure 5. Stroke outcomes and flow cytometry in CD200 Fc treated mice. (A) Representative images of brain slices stained with cresyl violet (CV) and quantification of brain infarct volumes, NDS, and corner test scores in CD200Fc vs. IgG treated mice. N=3 for sham and 6–7 for stroke per group; *P < 0.05. (B) Microglial expression of cell membrane markers CD68/CD206 measured as mean fluorescence intensity (MFI). (C) Microglial expression of intracellular markers IL-1β/TNFα. For (B) and (C), n=4/sham group and n=6/stroke group; *P < 0.05.

Article Snippet: Determination of CD200 and cytokine Levels The levels of CD200 in mouse brain, plasma and in the supernatants of primary neuronal culture were measured by a sandwich ELISA kit according to manufacture instruction ((# EK1185, Mouse CD200 PicoKine ELISA Kit, Boster Biological, Pleasanton, CA, USA.).

Techniques: Flow Cytometry, Staining, Expressing, Membrane, Fluorescence

Journal: Stroke

Article Title: Neuronal CD200 Signaling Is Protective in the Acute Phase of Ischemic Stroke.

doi: 10.1161/STROKEAHA.120.032374

Figure Lengend Snippet: Figure 6. Interaction of neuronal CD200 with lymphocytic CD200R1. (A) The purity of CD45+CD3+

Article Snippet: Determination of CD200 and cytokine Levels The levels of CD200 in mouse brain, plasma and in the supernatants of primary neuronal culture were measured by a sandwich ELISA kit according to manufacture instruction ((# EK1185, Mouse CD200 PicoKine ELISA Kit, Boster Biological, Pleasanton, CA, USA.).

Techniques:

Journal: Frontiers in Immunology

Article Title: Unveiling spatial complexity in solid tumor immune microenvironments through multiplexed imaging

doi: 10.3389/fimmu.2024.1383932

Figure Lengend Snippet: Immunophenotyping panel for multiplexed tissue imaging of cancer.

Article Snippet: CD200 , REA1067 , 50 , 130-118-128 , FITC , Miltenyi Biotec.

Techniques: Imaging