mr207821 Search Results


pcmv6 mouse rnf8  (OriGene)
90
OriGene pcmv6 mouse rnf8
(A) Predicted let-7 miRNA binding sites in <t>Rnf8</t> mRNA. (B–F) Western blot analysis and densitometric quantification of RNF8 protein (B, D, and F) and qRT-PCR of Rnf8 mRNA (C and E) in let7b/c2 +/+ and let7b/c2 ΔHep (B and C); EGFP and let-7 sponge AAV-transduced (D and E); EGFP and pre-let-7c-1 AAV-transduced (F) livers treated with HFD feeding. (G) 3′ UTR reporter assays in HepG2 cells transfected with Rnf8 wild-type or mutant 3′ UTR reporter constructs and a let-7c mimic expression vector. (H and I) Western blot analysis (H) and densitometric quantification (I) of RXRα expression in Rxra - and Rnf8 -transfected Hepa-1 cells. (J and K) Fold change of Rnf8 (J) and Rxra (K) mRNA by qRT-PCR analysis in Rxra - and Rnf8 -transfected Hepa-1 cells. (L) Western blot analysis and the densitometric quantification of RXRα in Rxra - and Rnf8- transfected Hepa-1 cells treated with the proteasome inhibitor MG-132. (M) Ubiquitination assays for Rxra - and Rnf8 -transfected and MG-132-treated Hepa-1 cells. RXRα was immunoprecipitated and polyubiquitin detected by anti-ubiquitin antibody. RXRα expression was confirmed in whole-cell lysate as input. (N) Scheme of 3-step inhibition for PPARα/RXRα pathway that the current study demonstrates.
Pcmv6 Mouse Rnf8, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mr207821/pmc08424520-277-5-7?v=OriGene
Average 90 stars, based on 1 article reviews
pcmv6 mouse rnf8 - by Bioz Stars, 2026-08
90/100 stars
  Buy from Supplier

Image Search Results


(A) Predicted let-7 miRNA binding sites in Rnf8 mRNA. (B–F) Western blot analysis and densitometric quantification of RNF8 protein (B, D, and F) and qRT-PCR of Rnf8 mRNA (C and E) in let7b/c2 +/+ and let7b/c2 ΔHep (B and C); EGFP and let-7 sponge AAV-transduced (D and E); EGFP and pre-let-7c-1 AAV-transduced (F) livers treated with HFD feeding. (G) 3′ UTR reporter assays in HepG2 cells transfected with Rnf8 wild-type or mutant 3′ UTR reporter constructs and a let-7c mimic expression vector. (H and I) Western blot analysis (H) and densitometric quantification (I) of RXRα expression in Rxra - and Rnf8 -transfected Hepa-1 cells. (J and K) Fold change of Rnf8 (J) and Rxra (K) mRNA by qRT-PCR analysis in Rxra - and Rnf8 -transfected Hepa-1 cells. (L) Western blot analysis and the densitometric quantification of RXRα in Rxra - and Rnf8- transfected Hepa-1 cells treated with the proteasome inhibitor MG-132. (M) Ubiquitination assays for Rxra - and Rnf8 -transfected and MG-132-treated Hepa-1 cells. RXRα was immunoprecipitated and polyubiquitin detected by anti-ubiquitin antibody. RXRα expression was confirmed in whole-cell lysate as input. (N) Scheme of 3-step inhibition for PPARα/RXRα pathway that the current study demonstrates.

Journal: Cell reports

Article Title: Feedback repression of PPARα signaling by Let-7 microRNA

doi: 10.1016/j.celrep.2021.109506

Figure Lengend Snippet: (A) Predicted let-7 miRNA binding sites in Rnf8 mRNA. (B–F) Western blot analysis and densitometric quantification of RNF8 protein (B, D, and F) and qRT-PCR of Rnf8 mRNA (C and E) in let7b/c2 +/+ and let7b/c2 ΔHep (B and C); EGFP and let-7 sponge AAV-transduced (D and E); EGFP and pre-let-7c-1 AAV-transduced (F) livers treated with HFD feeding. (G) 3′ UTR reporter assays in HepG2 cells transfected with Rnf8 wild-type or mutant 3′ UTR reporter constructs and a let-7c mimic expression vector. (H and I) Western blot analysis (H) and densitometric quantification (I) of RXRα expression in Rxra - and Rnf8 -transfected Hepa-1 cells. (J and K) Fold change of Rnf8 (J) and Rxra (K) mRNA by qRT-PCR analysis in Rxra - and Rnf8 -transfected Hepa-1 cells. (L) Western blot analysis and the densitometric quantification of RXRα in Rxra - and Rnf8- transfected Hepa-1 cells treated with the proteasome inhibitor MG-132. (M) Ubiquitination assays for Rxra - and Rnf8 -transfected and MG-132-treated Hepa-1 cells. RXRα was immunoprecipitated and polyubiquitin detected by anti-ubiquitin antibody. RXRα expression was confirmed in whole-cell lysate as input. (N) Scheme of 3-step inhibition for PPARα/RXRα pathway that the current study demonstrates.

Article Snippet: pSG5-mouse Rxra ( ) and pCMV6-mouse Rnf8 (Origene) expression plasmids were co-transfected into Hepa-1c1c7 cells (ATCC) using Lipofectamine 3000 (Thermo Fisher Scientific). pRK5-HA-Ubiquitin-WT (Addgene) were co-transfected for polyubiquitin type analysis.

Techniques: Binding Assay, Western Blot, Quantitative RT-PCR, Transfection, Mutagenesis, Construct, Expressing, Plasmid Preparation, Ubiquitin Proteomics, Immunoprecipitation, Inhibition

Journal: Cell reports

Article Title: Feedback repression of PPARα signaling by Let-7 microRNA

doi: 10.1016/j.celrep.2021.109506

Figure Lengend Snippet:

Article Snippet: pSG5-mouse Rxra ( ) and pCMV6-mouse Rnf8 (Origene) expression plasmids were co-transfected into Hepa-1c1c7 cells (ATCC) using Lipofectamine 3000 (Thermo Fisher Scientific). pRK5-HA-Ubiquitin-WT (Addgene) were co-transfected for polyubiquitin type analysis.

Techniques: Ubiquitin Proteomics, Virus, Recombinant, Protease Inhibitor, TaqMan microRNA Assay, Chromatin Immunoprecipitation, Knock-Out, Negative Control, Software