mr gene Search Results


gene exp enhanced gfp mr04097229 mr  (Thermo Fisher)
90
Thermo Fisher gene exp enhanced gfp mr04097229 mr
Gene Exp Enhanced Gfp Mr04097229 Mr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gene exp sev mr04269880 mr  (Thermo Fisher)
89
Thermo Fisher gene exp sev mr04269880 mr
Gene Exp Sev Mr04269880 Mr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gene exp gfp mr03989638 mr  (Thermo Fisher)
90
Thermo Fisher gene exp gfp mr03989638 mr
Gene Exp Gfp Mr03989638 Mr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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gene exp yfp mr04329676 mr  (Thermo Fisher)
90
Thermo Fisher gene exp yfp mr04329676 mr
Gene Exp Yfp Mr04329676 Mr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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gene exp luciferase mr03987587 mr  (Thermo Fisher)
90
Thermo Fisher gene exp luciferase mr03987587 mr
Cell viability and <t>luciferase</t> activities were measured in separate plates of MCF7-AREc32 cells exposed to the indicated doses of the noted HDAC inhibitors for 24 h. Each measured parameter is plotted as ±S.E.M of three independent experiments.
Gene Exp Luciferase Mr03987587 Mr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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gene exp sev sox2 mr04269881 mr  (Thermo Fisher)
85
Thermo Fisher gene exp sev sox2 mr04269881 mr
Characterization and reprogramming of donor-derived osteoblasts. (a) Example of donor bone chip in media (arrow) with osteoblasts emerging onto culture flask (asterisk). Osteoblasts were cultured in growth (b) or osteogenic induction media (c) and the resulting calcium deposits were stained with Alizarin Red S and imaged (scale bars = 30 μ m and inserts are at 1x). (d) The donor-derived osteoblasts were assayed with qRT-PCR for osteoblast markers, osteocalcin, osteopontin, RUNX2, BMP2, and COL1A1 and compared to a commercially available osteoblast cell line (NHOst). (e) qRT-PCR analysis of pluripotent gene expression, <t>Sox2</t> and Oct4, in the hOBs compared to neonatal BJ fibroblasts. ( ∗ P < 0.05). (f) Alkaline phosphatase staining of primary reprogramming plate of hOB-iPSC. (g) TRA-1-81 immunofluorescence of initial hOB-iPSC colony (scale bars = 30 μ m).
Gene Exp Sev Sox2 Mr04269881 Mr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gene exp sev klf4 mr04421256 mr  (Thermo Fisher)
87
Thermo Fisher gene exp sev klf4 mr04421256 mr
Characterization and reprogramming of donor-derived osteoblasts. (a) Example of donor bone chip in media (arrow) with osteoblasts emerging onto culture flask (asterisk). Osteoblasts were cultured in growth (b) or osteogenic induction media (c) and the resulting calcium deposits were stained with Alizarin Red S and imaged (scale bars = 30 μ m and inserts are at 1x). (d) The donor-derived osteoblasts were assayed with qRT-PCR for osteoblast markers, osteocalcin, osteopontin, RUNX2, BMP2, and COL1A1 and compared to a commercially available osteoblast cell line (NHOst). (e) qRT-PCR analysis of pluripotent gene expression, <t>Sox2</t> and Oct4, in the hOBs compared to neonatal BJ fibroblasts. ( ∗ P < 0.05). (f) Alkaline phosphatase staining of primary reprogramming plate of hOB-iPSC. (g) TRA-1-81 immunofluorescence of initial hOB-iPSC colony (scale bars = 30 μ m).
Gene Exp Sev Klf4 Mr04421256 Mr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 87/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gene exp sev cmyc mr04269876 mr  (Thermo Fisher)
88
Thermo Fisher gene exp sev cmyc mr04269876 mr
Characterization and reprogramming of donor-derived osteoblasts. (a) Example of donor bone chip in media (arrow) with osteoblasts emerging onto culture flask (asterisk). Osteoblasts were cultured in growth (b) or osteogenic induction media (c) and the resulting calcium deposits were stained with Alizarin Red S and imaged (scale bars = 30 μ m and inserts are at 1x). (d) The donor-derived osteoblasts were assayed with qRT-PCR for osteoblast markers, osteocalcin, osteopontin, RUNX2, BMP2, and COL1A1 and compared to a commercially available osteoblast cell line (NHOst). (e) qRT-PCR analysis of pluripotent gene expression, <t>Sox2</t> and Oct4, in the hOBs compared to neonatal BJ fibroblasts. ( ∗ P < 0.05). (f) Alkaline phosphatase staining of primary reprogramming plate of hOB-iPSC. (g) TRA-1-81 immunofluorescence of initial hOB-iPSC colony (scale bars = 30 μ m).
Gene Exp Sev Cmyc Mr04269876 Mr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 88 stars, based on 1 article reviews
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Image Search Results


Cell viability and luciferase activities were measured in separate plates of MCF7-AREc32 cells exposed to the indicated doses of the noted HDAC inhibitors for 24 h. Each measured parameter is plotted as ±S.E.M of three independent experiments.

Journal: PLoS ONE

Article Title: HDAC Inhibitors Increase NRF2-Signaling in Tumour Cells and Blunt the Efficacy of Co-Adminstered Cytotoxic Agents

doi: 10.1371/journal.pone.0114055

Figure Lengend Snippet: Cell viability and luciferase activities were measured in separate plates of MCF7-AREc32 cells exposed to the indicated doses of the noted HDAC inhibitors for 24 h. Each measured parameter is plotted as ±S.E.M of three independent experiments.

Article Snippet: For the real-time PCR analysis, the following pre-designed TaqMan probe sets in solution were used: AKR1C1 : Hs00413886; Luciferase : Mr03987587; 18 s ribosomal RNA: Hs03003631 (all from PerkinElmer Applied Biosystems).

Techniques: Luciferase

A & B , MCF7-AREc32 cells were exposed to the indicated doses of Pim1 inhibitor 2 (Pim1), NVP-AEW541 or SFN. After 24 h had elapsed, total RNA was prepared, reverse-transcribed to cDNA and levels of AKR1C1 and luciferase mRNA was measured by real-time PCR (A). Alternatively, whole-cell lysates were prepared from duplicate dishes of cells and blotted for the indicated proteins (B). GAPDH serves as a loading control. C, MCF7-AREc32 cells were treated with non-targeting (siNT) or NRF2 targeting (siNRF2) siRNAs and, 48 h later, they were exposed to different doses of the indicated chemicals. After 24 h had elapsed, mRNA was prepared, and the amount of AKR1C1 mRNA determined by real-time quantitative PCR D, MCF7-AREc32 cells were treated with non-targeting (siNT) or NRF2 targeting (siNRF2) siRNAs and, 48 h later, they were exposed to different doses of the indicated chemicals. Cell viability was assessed 72 h later. Data are presented as ±S.E.M of three independent experiments.

Journal: PLoS ONE

Article Title: HDAC Inhibitors Increase NRF2-Signaling in Tumour Cells and Blunt the Efficacy of Co-Adminstered Cytotoxic Agents

doi: 10.1371/journal.pone.0114055

Figure Lengend Snippet: A & B , MCF7-AREc32 cells were exposed to the indicated doses of Pim1 inhibitor 2 (Pim1), NVP-AEW541 or SFN. After 24 h had elapsed, total RNA was prepared, reverse-transcribed to cDNA and levels of AKR1C1 and luciferase mRNA was measured by real-time PCR (A). Alternatively, whole-cell lysates were prepared from duplicate dishes of cells and blotted for the indicated proteins (B). GAPDH serves as a loading control. C, MCF7-AREc32 cells were treated with non-targeting (siNT) or NRF2 targeting (siNRF2) siRNAs and, 48 h later, they were exposed to different doses of the indicated chemicals. After 24 h had elapsed, mRNA was prepared, and the amount of AKR1C1 mRNA determined by real-time quantitative PCR D, MCF7-AREc32 cells were treated with non-targeting (siNT) or NRF2 targeting (siNRF2) siRNAs and, 48 h later, they were exposed to different doses of the indicated chemicals. Cell viability was assessed 72 h later. Data are presented as ±S.E.M of three independent experiments.

Article Snippet: For the real-time PCR analysis, the following pre-designed TaqMan probe sets in solution were used: AKR1C1 : Hs00413886; Luciferase : Mr03987587; 18 s ribosomal RNA: Hs03003631 (all from PerkinElmer Applied Biosystems).

Techniques: Reverse Transcription, Luciferase, Real-time Polymerase Chain Reaction, Control

A – G , MCF7-AREc32 cells were cultured for 24 h in the presence of the indicated concentrations of the stated HDAC inhibitors. The expression levels of AKR1C1 and Luciferase mRNAs (relative to vehicle control) was determined by real-time quantitative PCR. Data are plotted as ±S.E.M of three independent experiments.

Journal: PLoS ONE

Article Title: HDAC Inhibitors Increase NRF2-Signaling in Tumour Cells and Blunt the Efficacy of Co-Adminstered Cytotoxic Agents

doi: 10.1371/journal.pone.0114055

Figure Lengend Snippet: A – G , MCF7-AREc32 cells were cultured for 24 h in the presence of the indicated concentrations of the stated HDAC inhibitors. The expression levels of AKR1C1 and Luciferase mRNAs (relative to vehicle control) was determined by real-time quantitative PCR. Data are plotted as ±S.E.M of three independent experiments.

Article Snippet: For the real-time PCR analysis, the following pre-designed TaqMan probe sets in solution were used: AKR1C1 : Hs00413886; Luciferase : Mr03987587; 18 s ribosomal RNA: Hs03003631 (all from PerkinElmer Applied Biosystems).

Techniques: Cell Culture, Expressing, Luciferase, Control, Real-time Polymerase Chain Reaction

A, Duplicate dishes of A-431 cells were treated with CI-994, Ent or vehicle control for 24 h and blotted for the indicated proteins. B – F, MCF7-AREc32 cells were treated with non-targeting (siNT) or NRF2 targeting (siNRF2) siRNAs and, 48 h later, they were exposed to different doses of the indicated chemicals for 24 h. The expression level of Luciferase (B), AKR1C1 (C) or AKR1C3 (D) was determined by real-time quantitative PCR. Protein levels (E & F) were determined by immunoblot. Data are presented as ±S.E.M of three independent experiments (B – D).

Journal: PLoS ONE

Article Title: HDAC Inhibitors Increase NRF2-Signaling in Tumour Cells and Blunt the Efficacy of Co-Adminstered Cytotoxic Agents

doi: 10.1371/journal.pone.0114055

Figure Lengend Snippet: A, Duplicate dishes of A-431 cells were treated with CI-994, Ent or vehicle control for 24 h and blotted for the indicated proteins. B – F, MCF7-AREc32 cells were treated with non-targeting (siNT) or NRF2 targeting (siNRF2) siRNAs and, 48 h later, they were exposed to different doses of the indicated chemicals for 24 h. The expression level of Luciferase (B), AKR1C1 (C) or AKR1C3 (D) was determined by real-time quantitative PCR. Protein levels (E & F) were determined by immunoblot. Data are presented as ±S.E.M of three independent experiments (B – D).

Article Snippet: For the real-time PCR analysis, the following pre-designed TaqMan probe sets in solution were used: AKR1C1 : Hs00413886; Luciferase : Mr03987587; 18 s ribosomal RNA: Hs03003631 (all from PerkinElmer Applied Biosystems).

Techniques: Control, Expressing, Luciferase, Real-time Polymerase Chain Reaction, Western Blot

Characterization and reprogramming of donor-derived osteoblasts. (a) Example of donor bone chip in media (arrow) with osteoblasts emerging onto culture flask (asterisk). Osteoblasts were cultured in growth (b) or osteogenic induction media (c) and the resulting calcium deposits were stained with Alizarin Red S and imaged (scale bars = 30 μ m and inserts are at 1x). (d) The donor-derived osteoblasts were assayed with qRT-PCR for osteoblast markers, osteocalcin, osteopontin, RUNX2, BMP2, and COL1A1 and compared to a commercially available osteoblast cell line (NHOst). (e) qRT-PCR analysis of pluripotent gene expression, Sox2 and Oct4, in the hOBs compared to neonatal BJ fibroblasts. ( ∗ P < 0.05). (f) Alkaline phosphatase staining of primary reprogramming plate of hOB-iPSC. (g) TRA-1-81 immunofluorescence of initial hOB-iPSC colony (scale bars = 30 μ m).

Journal: Stem Cells International

Article Title: Preferential Lineage-Specific Differentiation of Osteoblast-Derived Induced Pluripotent Stem Cells into Osteoprogenitors

doi: 10.1155/2017/1513281

Figure Lengend Snippet: Characterization and reprogramming of donor-derived osteoblasts. (a) Example of donor bone chip in media (arrow) with osteoblasts emerging onto culture flask (asterisk). Osteoblasts were cultured in growth (b) or osteogenic induction media (c) and the resulting calcium deposits were stained with Alizarin Red S and imaged (scale bars = 30 μ m and inserts are at 1x). (d) The donor-derived osteoblasts were assayed with qRT-PCR for osteoblast markers, osteocalcin, osteopontin, RUNX2, BMP2, and COL1A1 and compared to a commercially available osteoblast cell line (NHOst). (e) qRT-PCR analysis of pluripotent gene expression, Sox2 and Oct4, in the hOBs compared to neonatal BJ fibroblasts. ( ∗ P < 0.05). (f) Alkaline phosphatase staining of primary reprogramming plate of hOB-iPSC. (g) TRA-1-81 immunofluorescence of initial hOB-iPSC colony (scale bars = 30 μ m).

Article Snippet: All qPCR was performed with Taq-man probes (Thermofisher, Sox 2; Hs01053049_s1, Oct4 (POU5F1); Hs00999634_gH, NANOG; Hs04260366_g1, hTERT; Hs00972656_m1, BMP7; Hs00233476_m1, Actin A2; Hs00426835_g1, PPAR γ ; Hs01115513_m1, LEP; Hs00174877_m1, LPL; Hs00173425_m1, ADIPOQ; Hs00605917_m1, osteocalcin (BGLAP); Hs01587814_g1, Osteopontin (GZMB); Hs01554355_m1, RUNX2; Hs00231692_m1, BMP4; Hs03676628_s1, COMP; Hs00164359_m1, ACAN; Hs00153936_m1, COL10A1; Hs00166657_m1, Sendai Virus Detection; Mr04269876_mr, Mr04269878_mr, Mr04269879_mr, Mr04269880_mr, and Mr04269881_mr).

Techniques: Derivative Assay, Cell Culture, Staining, Quantitative RT-PCR, Gene Expression, Immunofluorescence

Pluripotent marker expression analysis of hOB-iPSCs and hFB-iPSCs. (a) hOB-iPSC and hFB-iPSC qRT-PCR expression of endogenous pluripotent genes, Sox2, Oct4, Nanog, and hTERT ( ∗ P < 0.01). (b) Immunofluorescence of hOB-iPSC for pluripotency markers, TRA-1-60, Oct4, Nanog, Sox2, and IgG-FITC control (scale bars = 30 μ m). ( ∗ indicates nonstaining MEF background.)

Journal: Stem Cells International

Article Title: Preferential Lineage-Specific Differentiation of Osteoblast-Derived Induced Pluripotent Stem Cells into Osteoprogenitors

doi: 10.1155/2017/1513281

Figure Lengend Snippet: Pluripotent marker expression analysis of hOB-iPSCs and hFB-iPSCs. (a) hOB-iPSC and hFB-iPSC qRT-PCR expression of endogenous pluripotent genes, Sox2, Oct4, Nanog, and hTERT ( ∗ P < 0.01). (b) Immunofluorescence of hOB-iPSC for pluripotency markers, TRA-1-60, Oct4, Nanog, Sox2, and IgG-FITC control (scale bars = 30 μ m). ( ∗ indicates nonstaining MEF background.)

Article Snippet: All qPCR was performed with Taq-man probes (Thermofisher, Sox 2; Hs01053049_s1, Oct4 (POU5F1); Hs00999634_gH, NANOG; Hs04260366_g1, hTERT; Hs00972656_m1, BMP7; Hs00233476_m1, Actin A2; Hs00426835_g1, PPAR γ ; Hs01115513_m1, LEP; Hs00174877_m1, LPL; Hs00173425_m1, ADIPOQ; Hs00605917_m1, osteocalcin (BGLAP); Hs01587814_g1, Osteopontin (GZMB); Hs01554355_m1, RUNX2; Hs00231692_m1, BMP4; Hs03676628_s1, COMP; Hs00164359_m1, ACAN; Hs00153936_m1, COL10A1; Hs00166657_m1, Sendai Virus Detection; Mr04269876_mr, Mr04269878_mr, Mr04269879_mr, Mr04269880_mr, and Mr04269881_mr).

Techniques: Marker, Expressing, Quantitative RT-PCR, Immunofluorescence, Control

Induced osteoprogenitors (iOPs) cells differentiated from hOB-iPSC. (a) Flow cytometry analysis of iOPs and hMSCs revealed that the cells were positive for mesenchymal markers (CD29, CD44, CD90, CD105, and CD166) and negative for hematopoietic markers (CD14, CD31, and CD45) (black: CD marker expression, red: isotype control, and blue: unstained control). (b) Significantly ( P < 0.05) faster growth kinetics of the hOB-iOPs and hFB-iOPs, as compared to hOBs and hMSCs in either 20% serum-containing media or Xenofree StemPro media at all time points. (c) Differentiation time of hOB-iPSCs to iOPs was assayed using flow cytometry for mesenchymal cell markers, CD44 and CD105. (d) iOPs were found to have similar levels of osteogenic related genes, RUNX2, BMP2, osteocalcin, and COL1A1 as compared to hMSCs ∗ indicates significant difference from the hOB-iOPs ( P < 0.05). iOPs downregulated all pluripotency genes, Oct4, Sox2, Nanog, and hTERT indicating successful differentiations. ∗ indicates significant difference from the iPSC ( P < 0.05).

Journal: Stem Cells International

Article Title: Preferential Lineage-Specific Differentiation of Osteoblast-Derived Induced Pluripotent Stem Cells into Osteoprogenitors

doi: 10.1155/2017/1513281

Figure Lengend Snippet: Induced osteoprogenitors (iOPs) cells differentiated from hOB-iPSC. (a) Flow cytometry analysis of iOPs and hMSCs revealed that the cells were positive for mesenchymal markers (CD29, CD44, CD90, CD105, and CD166) and negative for hematopoietic markers (CD14, CD31, and CD45) (black: CD marker expression, red: isotype control, and blue: unstained control). (b) Significantly ( P < 0.05) faster growth kinetics of the hOB-iOPs and hFB-iOPs, as compared to hOBs and hMSCs in either 20% serum-containing media or Xenofree StemPro media at all time points. (c) Differentiation time of hOB-iPSCs to iOPs was assayed using flow cytometry for mesenchymal cell markers, CD44 and CD105. (d) iOPs were found to have similar levels of osteogenic related genes, RUNX2, BMP2, osteocalcin, and COL1A1 as compared to hMSCs ∗ indicates significant difference from the hOB-iOPs ( P < 0.05). iOPs downregulated all pluripotency genes, Oct4, Sox2, Nanog, and hTERT indicating successful differentiations. ∗ indicates significant difference from the iPSC ( P < 0.05).

Article Snippet: All qPCR was performed with Taq-man probes (Thermofisher, Sox 2; Hs01053049_s1, Oct4 (POU5F1); Hs00999634_gH, NANOG; Hs04260366_g1, hTERT; Hs00972656_m1, BMP7; Hs00233476_m1, Actin A2; Hs00426835_g1, PPAR γ ; Hs01115513_m1, LEP; Hs00174877_m1, LPL; Hs00173425_m1, ADIPOQ; Hs00605917_m1, osteocalcin (BGLAP); Hs01587814_g1, Osteopontin (GZMB); Hs01554355_m1, RUNX2; Hs00231692_m1, BMP4; Hs03676628_s1, COMP; Hs00164359_m1, ACAN; Hs00153936_m1, COL10A1; Hs00166657_m1, Sendai Virus Detection; Mr04269876_mr, Mr04269878_mr, Mr04269879_mr, Mr04269880_mr, and Mr04269881_mr).

Techniques: Flow Cytometry, Marker, Expressing, Control