mps Search Results


microwave  (Revvity)
92
Revvity microwave
Microwave, supplied by Revvity, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 92 stars, based on 1 article reviews
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mps autosampler  (Gerstel GmbH)
96
Gerstel GmbH mps autosampler
Mps Autosampler, supplied by Gerstel GmbH, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
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hyq pf hyclone sh30333 01  (Danaher Inc)
86
Danaher Inc hyq pf hyclone sh30333 01
Hyq Pf Hyclone Sh30333 01, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 86 stars, based on 1 article reviews
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hermetic nextron mps chh  (Nextron Corporation)
94
Nextron Corporation hermetic nextron mps chh
Hermetic Nextron Mps Chh, supplied by Nextron Corporation, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mps vi  (Biosynth Carbosynth)
90
Biosynth Carbosynth mps vi
Mps Vi, supplied by Biosynth Carbosynth, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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anti rps27  (Proteintech)
92
Proteintech anti rps27
Anti Rps27, supplied by Proteintech, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 92 stars, based on 1 article reviews
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mps 1000 plate centrifuge  (Labnet International Inc)
93
Labnet International Inc mps 1000 plate centrifuge
Mps 1000 Plate Centrifuge, supplied by Labnet International Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
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mps  (World Precision Instruments)
94
World Precision Instruments mps
Mps, supplied by World Precision Instruments, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
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mps1 n terminal gfp crispr  (Addgene inc)
90
Addgene inc mps1 n terminal gfp crispr
Figure 1. <t>MPS1</t> can localize to end-on attached kinetochores (A) Schematic diagram depicting transient MPS1 binding to microtubule-attached kinetochores. (B) Nocodazole (Noc) or MG132 arrested HeLa <t>MPS1-GFP</t> cells were either directly fixed or cooled to 4C for 9 min then fixed and immunostained as indicated. (C) MPS1 kinetochore cell average intensities of cells in (B). Bars show mean ± SEM. ****p % 0.0001. (D) STLC-arrested HeLa MPS1-GFP cells treated as in (B). The K-fiber marker HURP was used to visualize kinetochore attachments.19,20
Mps1 N Terminal Gfp Crispr, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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parallel sequencing mps  (Illumina Inc)
96
Illumina Inc parallel sequencing mps
Figure 1. <t>MPS1</t> can localize to end-on attached kinetochores (A) Schematic diagram depicting transient MPS1 binding to microtubule-attached kinetochores. (B) Nocodazole (Noc) or MG132 arrested HeLa <t>MPS1-GFP</t> cells were either directly fixed or cooled to 4C for 9 min then fixed and immunostained as indicated. (C) MPS1 kinetochore cell average intensities of cells in (B). Bars show mean ± SEM. ****p % 0.0001. (D) STLC-arrested HeLa MPS1-GFP cells treated as in (B). The K-fiber marker HURP was used to visualize kinetochore attachments.19,20
Parallel Sequencing Mps, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/parallel sequencing mps/product/Illumina Inc
Average 96 stars, based on 1 article reviews
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mbt 6 903 mps library  (Bruker Corporation)
99
Bruker Corporation mbt 6 903 mps library
Figure 1. <t>MPS1</t> can localize to end-on attached kinetochores (A) Schematic diagram depicting transient MPS1 binding to microtubule-attached kinetochores. (B) Nocodazole (Noc) or MG132 arrested HeLa <t>MPS1-GFP</t> cells were either directly fixed or cooled to 4C for 9 min then fixed and immunostained as indicated. (C) MPS1 kinetochore cell average intensities of cells in (B). Bars show mean ± SEM. ****p % 0.0001. (D) STLC-arrested HeLa MPS1-GFP cells treated as in (B). The K-fiber marker HURP was used to visualize kinetochore attachments.19,20
Mbt 6 903 Mps Library, supplied by Bruker Corporation, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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calibration  (Sartorius AG)
91
Sartorius AG calibration
Figure 1. <t>MPS1</t> can localize to end-on attached kinetochores (A) Schematic diagram depicting transient MPS1 binding to microtubule-attached kinetochores. (B) Nocodazole (Noc) or MG132 arrested HeLa <t>MPS1-GFP</t> cells were either directly fixed or cooled to 4C for 9 min then fixed and immunostained as indicated. (C) MPS1 kinetochore cell average intensities of cells in (B). Bars show mean ± SEM. ****p % 0.0001. (D) STLC-arrested HeLa MPS1-GFP cells treated as in (B). The K-fiber marker HURP was used to visualize kinetochore attachments.19,20
Calibration, supplied by Sartorius AG, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Figure 1. MPS1 can localize to end-on attached kinetochores (A) Schematic diagram depicting transient MPS1 binding to microtubule-attached kinetochores. (B) Nocodazole (Noc) or MG132 arrested HeLa MPS1-GFP cells were either directly fixed or cooled to 4C for 9 min then fixed and immunostained as indicated. (C) MPS1 kinetochore cell average intensities of cells in (B). Bars show mean ± SEM. ****p % 0.0001. (D) STLC-arrested HeLa MPS1-GFP cells treated as in (B). The K-fiber marker HURP was used to visualize kinetochore attachments.19,20

Journal: Current biology : CB

Article Title: MPS1 localizes to end-on microtubule-attached kinetochores to promote microtubule release.

doi: 10.1016/j.cub.2022.10.047

Figure Lengend Snippet: Figure 1. MPS1 can localize to end-on attached kinetochores (A) Schematic diagram depicting transient MPS1 binding to microtubule-attached kinetochores. (B) Nocodazole (Noc) or MG132 arrested HeLa MPS1-GFP cells were either directly fixed or cooled to 4C for 9 min then fixed and immunostained as indicated. (C) MPS1 kinetochore cell average intensities of cells in (B). Bars show mean ± SEM. ****p % 0.0001. (D) STLC-arrested HeLa MPS1-GFP cells treated as in (B). The K-fiber marker HURP was used to visualize kinetochore attachments.19,20

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER siRNA against HEC1 5’UTR (5’-CCCUGGGUCGUGUCAGGAA-3’) Nijenhuis et al.12 N/A siRNA against BUBR1 3’UTR (5’-GCAATCAAGTCTCACAGAT-3’) Espert et al.24 N/A On-target plus Smartpool siRNA oligos against PPP2R5A (5’-GCUCAAAGAUGCCACUUCA-3’, 5’-CAAUACAAGUGCCGAAUAA-3’, 5’-UGAAUGAACUGGUUGAGUA-3’, 5’-GGAAAUGAAUGGCAAGCUU-3’) Dharmacon Cat#L-009352 On-target plus Smartpool siRNA oligos against PPP2R5B (5’-CGCAUGAUCUCAGUGAAUA-3’, 5’-UCAAGUCGCUGUCUGUCUU-3’, 5’-CAAACCAUCGUAUCACUGA-3’, 5’-GAACAAUGAGUAUAUCCUA-3’) Dharmacon Cat#L-009366 On-target plus Smartpool siRNA oligos against PPP2R5C (5’-GGAUUUGCCUUACCACUAA-3’, 5’-GGAAGAUGAACCAACGUUA-3’, 5’-CAUCAGAAUUUGUGAAGAU-3’, 5’-CAGAAGUAGUCCAUAUGUU-3’) Dharmacon Cat#L-009433 On-target plus Smartpool siRNA oligos against PPP2R5D (5’-GUACAUCGACCAGAAGUUU-3’, 5’-UCCAUGGACUGAUCUAUAA-3’, 5’-UGACUGAGCCGGUAAUUGU-3’, 5’-GUAGGCAGAUCAACCACAU-3’) Dharmacon Cat#L-009799 On-target plus Smartpool siRNA oligos against PPP2R5E (5’-UUAAUGAACUGGUGGACUA-3’, 5’-GCACAGCUGGCAUAUUGUA-3’, 5’-GACACGCUAUCUGAUCUUA-3’, 5’-GGAUAAAGUAGACGGAUUU-3’) Dharmacon Cat#L-008531 Recombinant DNA Plasmid: MPS1 N-terminal GFP CRISPR This paper N/A Plasmid: AAVS1CRISPR_inducible miRFKBP5_Mis12-GFP-FKBP3:mCherryFRB-INCENP 47-918 This paper, adapted from plasmids pERB109 and pMK232 (Addgene #28280 and #72834) Ballister et al.30; Natsume et al.36 N/A Plasmids: pcDNA5-mCherry-BUBR1 WT, LI/AA Espert et al.24 N/A Plasmids: pcDNA5-GFP-MPS1 WT, KD, N8A, N8E This paper and Hayward et al.10 N/A Plasmids: pcDNA5-HEC1-mCherryWT, 9A, 9D This paper N/A Plasmid: pcDNA5-GFP-B56 Delta This paper N/A Software and algorithms FIJI distribution of ImageJ v1.52q-v1.53s NIH image; Schindelin et al.37 https://imagej.nih.gov/ij/; RRID: SCR_003070 R v4.0.0 R Core Team http://www.r-project.org; RRID: SCR_001905 RStudio v1.3.959 RStudio Team38 http://www.rstudio.com; RRID: SCR_000432 (Continued on next page) ll OPEN ACCESS Current Biology 32, 5200–5208.e1–e8, December 5, 2022 e3 Report

Techniques: Binding Assay, Marker

Figure 3. Aurora B-dependent localization of MPS1 to kinetochores can be uncoupled from the microtubule-attachment status (A) Diagram of the rapamycin-dependent dimerization module used to recruit INCENP-Aurora B to the kinetochore protein Mis12. (B) HeLa MPS1-GFP cells expressing the Aurora B kinetochore targeting system were arrested with nocodazole or MG132 , then treated with rapamycin for 2 min as indicated and stained for Aurora B-pT232. (C) Plot of Aurora B-pT232 centromere and kinetochore intensities. Bars represent mean ± SEM. ****p % 0.0001. (D) Movie stills of HeLa MPS1-GFP cells expressing the Aurora B kinetochore targeting system treated with MG132. Cell at 1 min is at metaphase prior to the addition of rapamycin. Tubulin and DNA were visualized with SiR-Tubulin and Hoechst.

Journal: Current biology : CB

Article Title: MPS1 localizes to end-on microtubule-attached kinetochores to promote microtubule release.

doi: 10.1016/j.cub.2022.10.047

Figure Lengend Snippet: Figure 3. Aurora B-dependent localization of MPS1 to kinetochores can be uncoupled from the microtubule-attachment status (A) Diagram of the rapamycin-dependent dimerization module used to recruit INCENP-Aurora B to the kinetochore protein Mis12. (B) HeLa MPS1-GFP cells expressing the Aurora B kinetochore targeting system were arrested with nocodazole or MG132 , then treated with rapamycin for 2 min as indicated and stained for Aurora B-pT232. (C) Plot of Aurora B-pT232 centromere and kinetochore intensities. Bars represent mean ± SEM. ****p % 0.0001. (D) Movie stills of HeLa MPS1-GFP cells expressing the Aurora B kinetochore targeting system treated with MG132. Cell at 1 min is at metaphase prior to the addition of rapamycin. Tubulin and DNA were visualized with SiR-Tubulin and Hoechst.

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER siRNA against HEC1 5’UTR (5’-CCCUGGGUCGUGUCAGGAA-3’) Nijenhuis et al.12 N/A siRNA against BUBR1 3’UTR (5’-GCAATCAAGTCTCACAGAT-3’) Espert et al.24 N/A On-target plus Smartpool siRNA oligos against PPP2R5A (5’-GCUCAAAGAUGCCACUUCA-3’, 5’-CAAUACAAGUGCCGAAUAA-3’, 5’-UGAAUGAACUGGUUGAGUA-3’, 5’-GGAAAUGAAUGGCAAGCUU-3’) Dharmacon Cat#L-009352 On-target plus Smartpool siRNA oligos against PPP2R5B (5’-CGCAUGAUCUCAGUGAAUA-3’, 5’-UCAAGUCGCUGUCUGUCUU-3’, 5’-CAAACCAUCGUAUCACUGA-3’, 5’-GAACAAUGAGUAUAUCCUA-3’) Dharmacon Cat#L-009366 On-target plus Smartpool siRNA oligos against PPP2R5C (5’-GGAUUUGCCUUACCACUAA-3’, 5’-GGAAGAUGAACCAACGUUA-3’, 5’-CAUCAGAAUUUGUGAAGAU-3’, 5’-CAGAAGUAGUCCAUAUGUU-3’) Dharmacon Cat#L-009433 On-target plus Smartpool siRNA oligos against PPP2R5D (5’-GUACAUCGACCAGAAGUUU-3’, 5’-UCCAUGGACUGAUCUAUAA-3’, 5’-UGACUGAGCCGGUAAUUGU-3’, 5’-GUAGGCAGAUCAACCACAU-3’) Dharmacon Cat#L-009799 On-target plus Smartpool siRNA oligos against PPP2R5E (5’-UUAAUGAACUGGUGGACUA-3’, 5’-GCACAGCUGGCAUAUUGUA-3’, 5’-GACACGCUAUCUGAUCUUA-3’, 5’-GGAUAAAGUAGACGGAUUU-3’) Dharmacon Cat#L-008531 Recombinant DNA Plasmid: MPS1 N-terminal GFP CRISPR This paper N/A Plasmid: AAVS1CRISPR_inducible miRFKBP5_Mis12-GFP-FKBP3:mCherryFRB-INCENP 47-918 This paper, adapted from plasmids pERB109 and pMK232 (Addgene #28280 and #72834) Ballister et al.30; Natsume et al.36 N/A Plasmids: pcDNA5-mCherry-BUBR1 WT, LI/AA Espert et al.24 N/A Plasmids: pcDNA5-GFP-MPS1 WT, KD, N8A, N8E This paper and Hayward et al.10 N/A Plasmids: pcDNA5-HEC1-mCherryWT, 9A, 9D This paper N/A Plasmid: pcDNA5-GFP-B56 Delta This paper N/A Software and algorithms FIJI distribution of ImageJ v1.52q-v1.53s NIH image; Schindelin et al.37 https://imagej.nih.gov/ij/; RRID: SCR_003070 R v4.0.0 R Core Team http://www.r-project.org; RRID: SCR_001905 RStudio v1.3.959 RStudio Team38 http://www.rstudio.com; RRID: SCR_000432 (Continued on next page) ll OPEN ACCESS Current Biology 32, 5200–5208.e1–e8, December 5, 2022 e3 Report

Techniques: Expressing, Staining