Journal: PLoS Pathogens

Article Title: Neutrophil Elastase Causes Tissue Damage That Decreases Host Tolerance to Lung Infection with Burkholderia Species

doi: 10.1371/journal.ppat.1004327

Figure Lengend Snippet: Mice were infected intranasaly with B. thailandensis (A, 5×10 4 CFU or B, 5×10 5 CFU) and their survival was monitored. (C, D) Mice infected with B. thailandensis (5×10 5 CFU) were sacrificed 72 h post-infection and the organs bacterial burden in the indicated tissues was measured. (E) Flow cytometric analysis of BAL cells and MPO and elastase activity levels in the lung homogenates obtained from the indicated mouse strains at 72 h p.i. ( n = 5 for all except Il-1a −/− n = 3). (F) The total area of the inflammatory nodules of lung sections stained with H&E was measured and expressed as percentage of the total lung lobe area ( n = 5). (G) IgM and collagen were measured in the BALF collected 72 h p.i. from mice ( n = 5) infected with B. thailandensis (5×10 5 CFU). Data are expressed as mean + S.D. * p <0.05, ** p <0.01. (A, B) log rank Kaplan-Meier test, (C–G) Mann-Whitney U test. DKO is Il-1r1 −/− /Il-18 −/− double deficient mouse. One representative experiment of three (A–C, F, G) or one (D–E) is shown.

Article Snippet: Myeloperoxidase or elastase activity were measured in lung homogenates using the MPO fluorometric detection kit (Enzo) or as previously described .

Techniques: Infection, Activity Assay, Staining, MANN-WHITNEY

Journal: PLoS Pathogens

Article Title: Neutrophil Elastase Causes Tissue Damage That Decreases Host Tolerance to Lung Infection with Burkholderia Species

doi: 10.1371/journal.ppat.1004327

Figure Lengend Snippet: (A) Mice were intranasaly infected with 5×10 5 CFU B. thailandensis and their survival was monitored. (B) Elastase inhibitor sivelestat (125 µg/mouse, i.n.) and serine protease inhibitor BBI (3 mg/mouse, oral gavage) were administered on day 1, 2, 3 p.i. to C57BL/6 intranasaly infected with 1×10 5 CFU B. thailandensis . (C) Flow cytometric analysis of BAL cells, levels of MPO activity in lung homogenates, and total area of the inflammatory nodules for mice infected with 5×10 5 CFU B. thailandensis ( n = 5). (D, E) Organs bacterial burden in the indicated tissues of mice infected with 5×10 5 CFU (D) or 10 6 CFU (E) B. thailandensis . (F) Wild type and Cxcr2 −/− mice ( n = 7, pool of two experiments) were intranasaly infected with 2×10 5 CFU B. thailandensis and survival and BALF myeloid cells composition (G) ( n = 4 C57BL/6J, n = 5 Cxcr2 −/− ) were measured. (H) Cxcr2 −/− mice ( n = 4) were intranasaly infected with 2×10 5 CFU B. thailandensis and their survival was monitored. C57BL/6J or Elane −/− neutrophils (1.5×10 6 ) were adoptively transferred by intravenous injection 24 and 96 hours p.i. Data are expressed as mean +S.D.* p <0.05, ** p <0.01. # p <0.05 Elane −/− respect to Nos2 −/− .(A, B, F, H) log rank Kaplan-Meier test, (C, D, E, G) Mann-Whitney U test. One representative experiment of three (A, C, D, E, G) or two (B, F, H) is shown.

Article Snippet: Myeloperoxidase or elastase activity were measured in lung homogenates using the MPO fluorometric detection kit (Enzo) or as previously described .

Techniques: Infection, Protease Inhibitor, Activity Assay, Injection, MANN-WHITNEY

Journal: International Journal of Molecular Sciences

Article Title: Mitigating the Effects of Xuebijing Injection on Hematopoietic Cell Injury Induced by Total Body Irradiation with γ rays by Decreasing Reactive Oxygen Species Levels

doi: 10.3390/ijms150610541

Figure Lengend Snippet: The effects of XBJ on the serum levels of GSH, SOD and MPO. Serum was collected from mice nine days after administration of 2Gy TBI, and the enzyme levels were measured with the appropriate kits. ( A ) GSH levels; ( B ) SOD levels; ( C ) myeloperoxidase (MPO) levels. The data were expressed as the means ± SD ( n = 5). ** p < 0.01 vs . the control group; ## p < 0.05 vs . the IR group.

Article Snippet: Glutathione (GSH), superoxide dismutase (SOD) and myeloperoxidase (MPO) detection kit instructions were purchased from ImmunoWay (Newark, DE, USA).

Techniques: Control

Journal: Veterinary Sciences

Article Title: Investigation of Anti-Myeloperoxidase Antibodies in a Dog with Bilateral Necrotizing Scleritis

doi: 10.3390/vetsci2030259

Figure Lengend Snippet: Results of the anti-MPO ELISA. A commercial kit developed to detect anti-MPO antibodies in human sera, was used to test our samples. We followed the manufacturer’s instructions, but used an HRP anti-dog IgG secondary antibody rather than the anti-human secondary provided with the kit. Absorbance values were obtained using a multi-function plate reader. Using the “average + 2 or 3 standard deviations” cut-off, our patient was not positive for anti-MPO antibodies, but one of the control dogs was.

Article Snippet: Two months after prednisolone discontinuation, serum samples from this dog and 13 healthy dogs were analyzed using a commercial anti-human myeloperoxidase (MPO) detection ELISA kit (Diamedix Inc., Miami Lakes, FL, USA).

Techniques: Enzyme-linked Immunosorbent Assay, Control