Journal: BMC Musculoskeletal Disorders

Article Title: Neutrophil extracellular traps induce the bone erosion of gout

doi: 10.1186/s12891-022-06115-w

Figure Lengend Snippet: MSU crystals induce the formation of NETs in vivo. a The concentration of DNA, NE, MPO in the MSU group was higher than the NC group on days 1, 7 and 14. * P < 0.05, ** P < 0.01, *** P < 0.001 as determined by t test ( n = 3). b The immunofluorescence of the joint flushing fluid (× 20) The formation of NETs (MPO + H3 + DNA + ) in group MSU was significantly higher than NC groups on days 1, 7 and 14. c The histopathology of tibia (× 100). There was slight inflammatory infiltration in the cartilage on 1 day, while that was relieved at 7 day and 14 days in the MSU group. d IL-1β、TNF-αwere measured with ELISA. The level of two of them were higher than NC group at 24 h, on the day 7 and 14, the comparison was no longer statistically significant. This result was consistent with c

Article Snippet: The levels of IL-1β(Dakewe Biotech, 1310122), TNF-α(Dakewe Biotech, 1317202), NE (Cusabio, CSB-E08847r), and MPO (Cusabio, CSB-E08722R) were detected using ELISA kit according to the manufacturer's protocol.

Techniques: In Vivo, Concentration Assay, Immunofluorescence, Histopathology, Enzyme-linked Immunosorbent Assay, Comparison

Journal: Kidney international

Article Title: Streptococcal pyrogenic exotoxin B antibodies in a mouse model of glomerulonephritis.

doi: 10.1038/sj.ki.5002407

Figure Lengend Snippet: Figure 5 | Leukocyte infiltration in the kidney of mice hyperimmunized with 28-kDa SPE B mutant protein C192S. BALB/ c mice were hyperimmunized four times with (a) BSA or (b) C192S. Leukocyte infiltration was detected using anti-MPO antibody with fluorescence microscopy (n ¼ 10 per group). (c) The mean integral fluorescent intensity of glomeruli was analyzed in 10 fields per mouse (n ¼ 10 mice). Original magnification 200.

Article Snippet: Leukocyte infiltration was detected using anti-MPO antibody (Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA), and then using FITC-conjugated antigoat IgG (Jackson ImmunoResearch Laboratories Inc.).

Techniques: Mutagenesis, Fluorescence, Microscopy

Journal: Cells

Article Title: Inhibiting TNAP Attenuates the Aortic Valve Calcification and Reduces Oxidative Stress

doi: 10.3390/cells15171543

Figure Lengend Snippet: Primary antibody and detection kits used in immunochemistry.

Article Snippet: All cross-sectioned heart-aortic valve regions were re-fixed in 10% neutral buffered formalin for 24 h. Representative paraffin sections were stained with Hematoxylin & Eosin (H&E) for general histopathology, von Kossa for calcium depositions, or ABC-based immunohistochemical analysis for TNAP (ABclonal, Woburn, MA, USA), ENPP (ABclonal, Woburn, MA, USA), cleaved caspase-3 (Cell signaling technology, Danvers, MA, USA), cleaved PARP (Santa cruz biotechnology, Dallas, TX, USA), MPO (ABclonal, Woburn, MA, USA), iNOS (Santa cruz biotechnology, Dallas, TX, USA), COX-2 (Cayman chemical, Ann Arbor, MI, USA), TNF-α (Santa cruz biotechnology, Dallas, TX, USA), IL-1β (Santa cruz biotechnology, Dallas, TX, USA), NT (Millipore, Burlington, MA, USA), 4-HNE (abcam, Cambridge, UK), TGF-β1 (Santa cruz biotechnology, Dallas, TX, USA), and α-SMA (ABclonal, Woburn, MA, USA).

Techniques: Plasmid Preparation

Journal: Cells

Article Title: Inhibiting TNAP Attenuates the Aortic Valve Calcification and Reduces Oxidative Stress

doi: 10.3390/cells15171543

Figure Lengend Snippet: Effects of TNAP inhibitors on oxidative stress, lipid peroxidation, and RNS signaling. ( A ) mRNA level of ROS response markers ( Nrf2 and NF-κB ). ( B ) Activity levels of antioxidant markers (SOD, GSH and CAT) in heart tissue. ( C ) Levels of lipid peroxidation markers (MDA, 4-HNE, and MPO) in heart tissue. ( D ) RNS signaling markers (iNOS and NT) in heart tissue. * p -value < 0.05.

Article Snippet: All cross-sectioned heart-aortic valve regions were re-fixed in 10% neutral buffered formalin for 24 h. Representative paraffin sections were stained with Hematoxylin & Eosin (H&E) for general histopathology, von Kossa for calcium depositions, or ABC-based immunohistochemical analysis for TNAP (ABclonal, Woburn, MA, USA), ENPP (ABclonal, Woburn, MA, USA), cleaved caspase-3 (Cell signaling technology, Danvers, MA, USA), cleaved PARP (Santa cruz biotechnology, Dallas, TX, USA), MPO (ABclonal, Woburn, MA, USA), iNOS (Santa cruz biotechnology, Dallas, TX, USA), COX-2 (Cayman chemical, Ann Arbor, MI, USA), TNF-α (Santa cruz biotechnology, Dallas, TX, USA), IL-1β (Santa cruz biotechnology, Dallas, TX, USA), NT (Millipore, Burlington, MA, USA), 4-HNE (abcam, Cambridge, UK), TGF-β1 (Santa cruz biotechnology, Dallas, TX, USA), and α-SMA (ABclonal, Woburn, MA, USA).

Techniques: Activity Assay