Journal: Pain Physician

Article Title: Evaluation of the Effect of Duration on the Efficacy of Pulsed Radiofrequency in an Animal Model of Neuropathic Pain

doi: 10.36076/ppj.2018.2.191

Figure Lengend Snippet: Fig. 1. Immunopositive nerve fibers for TNF-α of CCI rats after scarification.

Article Snippet: Paraformaldehyde-fixed paraffin-embedded sciatic nerve sections (5 mm thickness) were incubated with antibodies to TNF-α (MP6-XT22, Novus Biologicals, Littleton, CO) and IL-6 (20F3, Thermo Fisher Scientific, Waltham, MA).

Techniques:

Journal: STAR Protocols

Article Title: Protocol to isolate immune cells from mouse pancreatic lymph nodes and whole pancreas for mass cytometric analyses

doi: 10.1016/j.xpro.2022.101938

Figure Lengend Snippet:

Article Snippet: Anti-mouse TNFα, Clone MP6-XT22, 1:50 dilution , Fluidigm , Cat#3162002C, Label 162Dy.

Techniques: Recombinant, Staining, Red Blood Cell Lysis, Software, Cytometry

Journal: Experimental cell research

Article Title: Using phage-assisted continuous evolution (PACE) to evolve human PD1.

doi: 10.1016/j.yexcr.2020.112244

Figure Lengend Snippet: Fig. 3. Summarization of PD1 variants that bind PDL1. (A) Under high levels of mutagenesis (MP6) during the 288 h of PACE. The Red line indicates the phage titer of each time point. And the grey line indicates the flow velocity of system. (B) During 288 h of evolution, “lagoon” samples were sequenced using the Sanger method, and 11 different preponderant oligotypes were identified which contained 9 amino acid sites. (C) Among these 11 oligotypes, 9 amino acids exhibited diverse retention from 9 times of T98L to 3 times G18A. (D) The summarization of 11 oligotypes is shown on a time axis.

Article Snippet: SP098, pAB107a, MP6 plasmid, S2060 and S2208 were obtained from Addgene.

Techniques: Mutagenesis