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OriGene
tm4sf5  Tm4sf5, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/product/mouse+tm4sf5/Tm4sf5+(NM_029360)+Mouse+Tagged+ORF+Clone/pmc08576418-61-5-17 Average 90 stars, based on 1 article reviews
tm4sf5 - by Bioz Stars,
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OriGene
tm4sf5 nm 029360 mouse orf  Tm4sf5 Nm 029360 Mouse Orf, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/product/mouse+tm4sf5/Tm4sf5+(NM_029360)+Mouse+Tagged+ORF+Clone/pm34187836-74-11-17 Average 90 stars, based on 1 article reviews
tm4sf5 nm 029360 mouse orf - by Bioz Stars,
2026-10
90/100 stars
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Lenti ORF clone of Tm4sf5 mGFP tagged Mouse transmembrane 4 superfamily member 5 Tm4sf5
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Lenti ORF particles Tm4sf5 Myc DDK tagged Mouse transmembrane 4 superfamily member 5 Tm4sf5 200ul 10 7 TU mL
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Image Search Results
Journal: Diabetes
Article Title: TM4SF5 Knockout Protects Mice From Diet-Induced Obesity Partly by Regulating Autophagy in Adipose Tissue
doi: 10.2337/db21-0145
Figure Lengend Snippet: TM4SF5 KO induces autophagy by inhibition of mTOR signaling. A and B : Immunoblot analysis of p-mTOR (Ser2481), mTOR, p-AMPK (Thr172), AMPK, p-ULK1 (Ser555, Ser757), LC3, SQSTM1/p62, p-HSL (Ser660), HSL, and ATGL proteins in BAT and iWAT of WT and TM4SF5 KO mice. Data represent the mean ± SEM of six mice per group (unpaired, two-tailed t test, ** P < 0.01, *** P < 0.001).
Article Snippet: To overexpress GFP- or Myc-fused
Techniques: Inhibition, Western Blot, Two Tailed Test
Journal: Diabetes
Article Title: TM4SF5 Knockout Protects Mice From Diet-Induced Obesity Partly by Regulating Autophagy in Adipose Tissue.
doi: 10.2337/db21-0145
Figure Lengend Snippet: Figure 1—TM4SF5 expression is abundant in subcutaneous WAT compared with BAT. A: qPCR analysis of Tm4sf5 gene expression in BAT, iWAT, epididymal WAT (eWAT), retroperitoneal WAT (rpWAT), perirenal WAT (prWAT), and perimetrial WAT (pmWAT) of mice. Data represent the mean ± SEM of six mice per group (unpaired, two-tailed t test, **P < 0.01, ***P < 0.001 vs. BAT). B: Immunoblot analysis of TM4SF5 protein in BAT and iWAT of mice. Data represent the mean ± SEM of six mice per group (unpaired, two-tailed t test, ***P < 0.001).
Article Snippet: To overexpress GFP- or Myc-fused TM4SF5 in C3H10T1/2 cells, we cloned
Techniques: Expressing, Gene Expression, Two Tailed Test, Western Blot
Journal: Diabetes
Article Title: TM4SF5 Knockout Protects Mice From Diet-Induced Obesity Partly by Regulating Autophagy in Adipose Tissue.
doi: 10.2337/db21-0145
Figure Lengend Snippet: Figure 2—TM4SF5 KO induces autophagy by inhibition of mTOR signaling. A and B: Immunoblot analysis of p-mTOR (Ser2481), mTOR, p-AMPK (Thr172), AMPK, p-ULK1 (Ser555, Ser757), LC3, SQSTM1/p62, p-HSL (Ser660), HSL, and ATGL proteins in BAT and iWAT of WT and TM4SF5 KO mice. Data represent the mean ± SEM of six mice per group (unpaired, two-tailed t test, **P < 0.01, ***P < 0.001).
Article Snippet: To overexpress GFP- or Myc-fused TM4SF5 in C3H10T1/2 cells, we cloned
Techniques: Inhibition, Western Blot, Two Tailed Test
Journal: Diabetes
Article Title: TM4SF5 Knockout Protects Mice From Diet-Induced Obesity Partly by Regulating Autophagy in Adipose Tissue.
doi: 10.2337/db21-0145
Figure Lengend Snippet: Figure 3—TM4SF5 regulates autophagy activation in adipocytes in vitro. Adipocytes were differentiated from preadipocytes obtained from iWAT of WT and TM4SF5 KO mice. A: Representative images of WT and TM4SF5 KO adipocytes immunostained with LC3 along with quantification and higher-magnification view of the boxed region. Neutral lipid and nuclei were counterstained with LipidTOX and DAPI. Size bar = 20 mm. B: Representative images of WT and TM4SF5 KO adipocytes immunostained with LC3 and PLIN1. Higher-magni- fication view of the boxed region is shown. Neutral lipid was counterstained with LipidTOX. Arrows indicate LC3-PLIN1 colocalized loca- tions. Size bar = 20 mm. C: Immunoblot analysis of p-mTOR (Ser2481), mTOR, p-ULK1 (Ser555, Ser757), SQSTM1/p62, p-HSL (Ser660), HSL, and ATGL proteins in WT and TM4SF5 KO adipocytes. D: Immunoblot analysis of LC3 in WT and TM4SF5 KO adipocytes after 6 and 24 h of chloroquine treatments (50 mmol/L). Data represent the mean ± SEM of six samples per group (unpaired, two-tailed t test, **P < 0.01, ***P < 0.001).
Article Snippet: To overexpress GFP- or Myc-fused TM4SF5 in C3H10T1/2 cells, we cloned
Techniques: Activation Assay, In Vitro, Western Blot, Two Tailed Test
Journal: Diabetes
Article Title: TM4SF5 Knockout Protects Mice From Diet-Induced Obesity Partly by Regulating Autophagy in Adipose Tissue.
doi: 10.2337/db21-0145
Figure Lengend Snippet: Figure 4—Overexpression of TM4SF5 downregulates autophagy and lipolysis. A: Representative image of C3H10T1/2 adipocytes over- expressing GFP-fused TM4SF5. Neutral lipid and lysosome were stained with LipidTOX and LysoTracker. Size bar = 20 mm. B: Represen- tative images of C3H10T1/2 adipocytes overexpressing Myc-fused TM4SF5 (OE) and mock control (Mock) stained with LC3 and PLIN1 along with quantification of LC3. Nuclei were counterstained with DAPI. Size bar = 20 mm. C: Immunoblot analysis of p-mTOR (Ser2481), mTOR, p-ULK1 (Ser555, Ser757), SQSTM1/p62, p-HSL (Ser660), HSL, and ATGL proteins in C3H10T1/2 adipocytes overexpressing TM4SF5 and mock controls treated with vehicle (CTL) or 5 mmol/L TSAHC for 24 h. Data represent the mean ± SEM of six samples per group (unpaired, two-tailed t test, **P < 0.01, ***P < 0.001).
Article Snippet: To overexpress GFP- or Myc-fused TM4SF5 in C3H10T1/2 cells, we cloned
Techniques: Over Expression, Expressing, Staining, Control, Western Blot, Two Tailed Test
Journal: Diabetes
Article Title: TM4SF5 Knockout Protects Mice From Diet-Induced Obesity Partly by Regulating Autophagy in Adipose Tissue.
doi: 10.2337/db21-0145
Figure Lengend Snippet: Figure 5—TM4SF5 KO mice manifest catabolic phenotype in adipose tissue. A: Immunoblot analysis of UCP1, COXIV, ATP5A, UQCRC2, MTCO1, SDHB, and NDUFB8 proteins in BAT and iWAT of WT and TM4SF5 KO mice. B: Representative transmission electron micros- copy images from BAT and iWAT of WT and TM4SF5 KO mice along with quantification of mitochondrial content (number of mitochondria per nucleus). Size bar = 2 mm. C: mtDNA content analysis relative to nuclear DNA (nDNA) in BAT and iWAT of WT and TM4SF5 KO mice. D: TTC assay in BAT and iWAT of WT and TM4SF5 KO mice. E: Representative images of paraffin sections from BAT and iWAT of WT and TM4SF5 KO mice immunostained with MCAD and LC3. Higher-magnification view of boxed region is shown. Colocalization was evalu- ated with use of ImageJ software via JACoP plugin, and PC values are presented. Size bar = 20 mm. F: qPCR analysis of Tm4sf5
Article Snippet: To overexpress GFP- or Myc-fused TM4SF5 in C3H10T1/2 cells, we cloned
Techniques: Western Blot, Transmission Assay, Software
Journal: Diabetes
Article Title: TM4SF5 Knockout Protects Mice From Diet-Induced Obesity Partly by Regulating Autophagy in Adipose Tissue.
doi: 10.2337/db21-0145
Figure Lengend Snippet: Figure 6—TM4SF5 regulates mitochondrial oxidative metabolism in adipocytes in vitro. A: Immunoblot analysis of UCP1, COXIV, ATP5A, UQCRC2, MTCO1, SDHB, and NDUFB8 proteins in WT and TM4SF5 KO adipocytes. Adipocytes were differentiated from preadipocytes obtained from BAT of WT and TM4SF5 KO mice. B: OCR analysis of WT and TM4SF5 adipocytes. Adipocytes were differentiated from preadipocytes obtained from iWAT of WT and TM4SF5 KO mice. C and D: Glycerol (C) and FFA (D) levels in conditioned media of WT and TM4SF5 KO adipocytes. Adipocytes were differentiated from preadipocytes obtained from iWAT of WT and TM4SF5 KO mice. E: Immu- noblot analysis of COXIV, ATP5A, UQCRC2, MTCO1, and NDUFB8 proteins in mock controls (Mock) and C3H10T1/2 adipocytes overex- pressing TM4SF5 (OE) treated with vehicle control (CTL) or TSAHC (5 mmol/L) for 24 h. F and G: Glycerol (F) and FFA (G) levels in conditioned media of mock controls and C3H10T1/2 adipocytes overexpressing TM4SF5. Data represent the mean ± SEM of six samples per group (unpaired, two-tailed t test, *P < 0.05, **P < 0.01, ***P < 0.001).
Article Snippet: To overexpress GFP- or Myc-fused TM4SF5 in C3H10T1/2 cells, we cloned
Techniques: In Vitro, Western Blot, Control, Two Tailed Test
Journal: Diabetes
Article Title: TM4SF5 Knockout Protects Mice From Diet-Induced Obesity Partly by Regulating Autophagy in Adipose Tissue.
doi: 10.2337/db21-0145
Figure Lengend Snippet: Figure 7—TM4SF5 KO protects mice from obesity-induced metabolic dysfunction. A: qPCR analysis of Tm4sf5 expression in BAT and iWAT of mice after 8 weeks of NCD or HFD feeding. B: Immunoblot analysis of TM4SF5 protein in iWAT of mice fed NCD or HFD for 8 weeks. C–E: Body weights (C), body fat and lean mass (D), and adipose tissue weight analysis (E) of WT and TM4SF5 KO mice fed with NCD and HFD for 8 weeks. F: Immunoblot analysis of p-mTOR (Ser2481), mTOR, UCP1, COXIV, LC3, SQSTM1/p62, PPARa, and F4/80 proteins in BAT and iWAT of WT and TM4SF5 KO mice fed with NCD and HFD for 8 weeks. G: Representative images of H-E–stained paraffin sections and LD size quantification from BAT and iWAT of WT and TM4SF5 KO mice fed with NCD or HFD for 8 weeks. Size bar = 100 mm. H: Immu- nofluorescence images of F4/80 in paraffin sections from iWAT of WT and TM4SF5 KO mice fed an HFD for 8 weeks. Size bar = 20 mm. Data represent the mean ± SEM of six mice per group (unpaired, two-tailed t test, *P < 0.05, **P < 0.01, ***P < 0.001). wks, weeks.
Article Snippet: To overexpress GFP- or Myc-fused TM4SF5 in C3H10T1/2 cells, we cloned
Techniques: Expressing, Western Blot, Staining, Two Tailed Test
Journal: Diabetes
Article Title: TM4SF5 Knockout Protects Mice From Diet-Induced Obesity Partly by Regulating Autophagy in Adipose Tissue.
doi: 10.2337/db21-0145
Figure Lengend Snippet: Figure 8—TM4SF5 KO restores insulin sensitivity in mice with HFD-induced obesity. WT and TM4SF5 KO mice were fed the NCD or HFD for 8 weeks. A and B: Intraperitoneal glucose tolerance test (A) and insulin sensitivity test (B). AUC, area under the curve. C and D: insulin (C) and triglyceride (TG), total cholesterol (TCHO), and glutamic-pyruvic transaminase (GPT) levels (D) in serum. E: Immunoblot analysis of p-AKT (Ser473), AKT, p-IRS1 (Ser612), IRS1 and p-IRS2 (Ser388), and IRS2 proteins in BAT and iWAT of WT and TM4SF5 KO mice fed HFD with acute insulin treatment (0.75 units/kg) and controls (CTL) without insulin treatment. F and G: Glycerol (F) and FFA (G) levels in serum of WT and TM4SF5 KO mice fed HFD with acute insulin treatment (0.75 units/kg) and controls (CTL) without insulin treatment. Data represent the mean ± SEM of six mice per group (unpaired, two-tailed t test, **P < 0.01, ***P < 0.001).
Article Snippet: To overexpress GFP- or Myc-fused TM4SF5 in C3H10T1/2 cells, we cloned
Techniques: Western Blot, Two Tailed Test