Journal:

Article Title: CHARACTERIZATION OF THE EXPRESSION PATTERN OF ADRENERGIC RECEPTORS IN RAT TASTE BUDS

doi: 10.1016/j.neuroscience.2010.05.021

Figure Lengend Snippet: List of primary antibodies used in investigation of adrenergic receptors in taste receptor cells.

Article Snippet: Digital photos of immunofluorescence cells were processed using Metamorph software. table ft1 table-wrap mode="anchored" t5 Table I caption a7 Antigen Immunogen Manufacturer, species, type, catalog number Dilution G α gust A peptide mapping within a highly divergent domain of G α gust of rat origin Santa Cruz Biotech, rabbit polyclonal, affinity purified IgG, sc-395 1:1000 1:5000 SNAP-25 Human crude synaptic immunoprecipitate that recognizes SNAP-25 protein Millipore, mouse monoclonal, MAB331 1:5000 NCAM Highly purified chicken NCAM Chemicon, rabbit polyclonal, affinity purified AB5032 1:2000 α 1A -AR Epitope mapping at the C-terminus of α 1A -AR of human origin Santa Cruz Biotech goat polyclonal, affinity purified IgG, sc-1477 1:500 1:3000 α 1B -AR Epitope corresponding to a peptide mapping at the C-terminus of α 1B adrenergic receptor of human origin, 94% homology with rat Santa Cruz Biotech, goat polyclonal, affinity purified IgG, sc-1476 1:500 α 1D -AR Epitope corresponding to a peptide mapping at the C-terminus of α1D adrenergic receptor of rat origin Santa Cruz Biotech, goat polyclonal, affinity purified IgG, sc-1475 1:200 α 2A -AR Synthetic peptide: C-TERRPNGLGPERS, corresponding to Internal sequence amino acids 246-258 of Human alpha 2a Adrenergic Receptor Abcam, goat polyclonal, affinity purified IgG, ab45871.

Techniques: Affinity Purification, Purification, Sequencing

Journal:

Article Title: CHARACTERIZATION OF THE EXPRESSION PATTERN OF ADRENERGIC RECEPTORS IN RAT TASTE BUDS

doi: 10.1016/j.neuroscience.2010.05.021

Figure Lengend Snippet: Primer Sequences used in RT-PCR reactions for adrenergic receptor subtypes.

Article Snippet: Digital photos of immunofluorescence cells were processed using Metamorph software. table ft1 table-wrap mode="anchored" t5 Table I caption a7 Antigen Immunogen Manufacturer, species, type, catalog number Dilution G α gust A peptide mapping within a highly divergent domain of G α gust of rat origin Santa Cruz Biotech, rabbit polyclonal, affinity purified IgG, sc-395 1:1000 1:5000 SNAP-25 Human crude synaptic immunoprecipitate that recognizes SNAP-25 protein Millipore, mouse monoclonal, MAB331 1:5000 NCAM Highly purified chicken NCAM Chemicon, rabbit polyclonal, affinity purified AB5032 1:2000 α 1A -AR Epitope mapping at the C-terminus of α 1A -AR of human origin Santa Cruz Biotech goat polyclonal, affinity purified IgG, sc-1477 1:500 1:3000 α 1B -AR Epitope corresponding to a peptide mapping at the C-terminus of α 1B adrenergic receptor of human origin, 94% homology with rat Santa Cruz Biotech, goat polyclonal, affinity purified IgG, sc-1476 1:500 α 1D -AR Epitope corresponding to a peptide mapping at the C-terminus of α1D adrenergic receptor of rat origin Santa Cruz Biotech, goat polyclonal, affinity purified IgG, sc-1475 1:200 α 2A -AR Synthetic peptide: C-TERRPNGLGPERS, corresponding to Internal sequence amino acids 246-258 of Human alpha 2a Adrenergic Receptor Abcam, goat polyclonal, affinity purified IgG, ab45871.

Techniques:

Journal:

Article Title: CHARACTERIZATION OF THE EXPRESSION PATTERN OF ADRENERGIC RECEPTORS IN RAT TASTE BUDS

doi: 10.1016/j.neuroscience.2010.05.021

Figure Lengend Snippet: Quantitative immunocytochemical single labeling patterns of alpha and beta receptors in taste receptor cells.

Article Snippet: Digital photos of immunofluorescence cells were processed using Metamorph software. table ft1 table-wrap mode="anchored" t5 Table I caption a7 Antigen Immunogen Manufacturer, species, type, catalog number Dilution G α gust A peptide mapping within a highly divergent domain of G α gust of rat origin Santa Cruz Biotech, rabbit polyclonal, affinity purified IgG, sc-395 1:1000 1:5000 SNAP-25 Human crude synaptic immunoprecipitate that recognizes SNAP-25 protein Millipore, mouse monoclonal, MAB331 1:5000 NCAM Highly purified chicken NCAM Chemicon, rabbit polyclonal, affinity purified AB5032 1:2000 α 1A -AR Epitope mapping at the C-terminus of α 1A -AR of human origin Santa Cruz Biotech goat polyclonal, affinity purified IgG, sc-1477 1:500 1:3000 α 1B -AR Epitope corresponding to a peptide mapping at the C-terminus of α 1B adrenergic receptor of human origin, 94% homology with rat Santa Cruz Biotech, goat polyclonal, affinity purified IgG, sc-1476 1:500 α 1D -AR Epitope corresponding to a peptide mapping at the C-terminus of α1D adrenergic receptor of rat origin Santa Cruz Biotech, goat polyclonal, affinity purified IgG, sc-1475 1:200 α 2A -AR Synthetic peptide: C-TERRPNGLGPERS, corresponding to Internal sequence amino acids 246-258 of Human alpha 2a Adrenergic Receptor Abcam, goat polyclonal, affinity purified IgG, ab45871.

Techniques: Labeling, Staining

Journal:

Article Title: CHARACTERIZATION OF THE EXPRESSION PATTERN OF ADRENERGIC RECEPTORS IN RAT TASTE BUDS

doi: 10.1016/j.neuroscience.2010.05.021

Figure Lengend Snippet: Quantitative immunocytochemical double labeling patterns of alpha and beta receptors with phenotypic taste receptor cell markers. Mean 1 represents the mean percentage of all adrenoceptor labeled cells which co-expressed the corresponding phenotypic marker per cross-sectioned taste bud. Mean 2 represents the mean percentage of all phenotypic marker labeled cells which co-expressed the corresponding adrenoceptor per cross-sectioned taste bud. Means are expressed ± SE.

Article Snippet: Digital photos of immunofluorescence cells were processed using Metamorph software. table ft1 table-wrap mode="anchored" t5 Table I caption a7 Antigen Immunogen Manufacturer, species, type, catalog number Dilution G α gust A peptide mapping within a highly divergent domain of G α gust of rat origin Santa Cruz Biotech, rabbit polyclonal, affinity purified IgG, sc-395 1:1000 1:5000 SNAP-25 Human crude synaptic immunoprecipitate that recognizes SNAP-25 protein Millipore, mouse monoclonal, MAB331 1:5000 NCAM Highly purified chicken NCAM Chemicon, rabbit polyclonal, affinity purified AB5032 1:2000 α 1A -AR Epitope mapping at the C-terminus of α 1A -AR of human origin Santa Cruz Biotech goat polyclonal, affinity purified IgG, sc-1477 1:500 1:3000 α 1B -AR Epitope corresponding to a peptide mapping at the C-terminus of α 1B adrenergic receptor of human origin, 94% homology with rat Santa Cruz Biotech, goat polyclonal, affinity purified IgG, sc-1476 1:500 α 1D -AR Epitope corresponding to a peptide mapping at the C-terminus of α1D adrenergic receptor of rat origin Santa Cruz Biotech, goat polyclonal, affinity purified IgG, sc-1475 1:200 α 2A -AR Synthetic peptide: C-TERRPNGLGPERS, corresponding to Internal sequence amino acids 246-258 of Human alpha 2a Adrenergic Receptor Abcam, goat polyclonal, affinity purified IgG, ab45871.

Techniques: Labeling, Marker

Journal:

Article Title: CHARACTERIZATION OF THE EXPRESSION PATTERN OF ADRENERGIC RECEPTORS IN RAT TASTE BUDS

doi: 10.1016/j.neuroscience.2010.05.021

Figure Lengend Snippet: Quantitative immunocytochemical double labeling patterns of alpha receptors with beta receptors in taste receptor Mean 1 represents the mean percentage of alpha adrenoceptor subtype labeled cells which co-expressed the β-2 receptor per cross-sectioned taste bud. Mean 2 represents the mean percentage of the β-2 receptor subtype labeled cells which co-expressed the corresponding alpha adrenoceptor per cross-sectioned taste bud. Means are expressed ± SE.

Article Snippet: Digital photos of immunofluorescence cells were processed using Metamorph software. table ft1 table-wrap mode="anchored" t5 Table I caption a7 Antigen Immunogen Manufacturer, species, type, catalog number Dilution G α gust A peptide mapping within a highly divergent domain of G α gust of rat origin Santa Cruz Biotech, rabbit polyclonal, affinity purified IgG, sc-395 1:1000 1:5000 SNAP-25 Human crude synaptic immunoprecipitate that recognizes SNAP-25 protein Millipore, mouse monoclonal, MAB331 1:5000 NCAM Highly purified chicken NCAM Chemicon, rabbit polyclonal, affinity purified AB5032 1:2000 α 1A -AR Epitope mapping at the C-terminus of α 1A -AR of human origin Santa Cruz Biotech goat polyclonal, affinity purified IgG, sc-1477 1:500 1:3000 α 1B -AR Epitope corresponding to a peptide mapping at the C-terminus of α 1B adrenergic receptor of human origin, 94% homology with rat Santa Cruz Biotech, goat polyclonal, affinity purified IgG, sc-1476 1:500 α 1D -AR Epitope corresponding to a peptide mapping at the C-terminus of α1D adrenergic receptor of rat origin Santa Cruz Biotech, goat polyclonal, affinity purified IgG, sc-1475 1:200 α 2A -AR Synthetic peptide: C-TERRPNGLGPERS, corresponding to Internal sequence amino acids 246-258 of Human alpha 2a Adrenergic Receptor Abcam, goat polyclonal, affinity purified IgG, ab45871.

Techniques: Labeling