Journal: PLoS ONE

Article Title: Fibroblasts Isolated from Human Middle Turbinate Mucosa Cause Neural Progenitor Cells to Differentiate into Glial Lineage Cells

doi: 10.1371/journal.pone.0076926

Figure Lengend Snippet: Two different morphologies of OECs were found. Both types were both GFAP and p75 NTR positive labeled. One type (A–D) with filamentous GFAP labeling (B) and punctate p75 NTR labeling (C). Arrow head identifies one cell with single p75 NTR labeling. Cells labeled only with p75 NTR were not counted as OECs in this study. Another type (E, F) had intense p75 NTR labeling (red) and diffuse GFAP labeling (green). Scale bars: 50 µm.

Article Snippet: The following primary antibodies were used: mouse anti-human Thy1.1 (1∶1200, Serotec), rabbit anti-human basic fibroblast growth factor (FGF2, 1∶100, Santa Cruz Biotechnology), mouse anti-human Nestin (1∶200, Chemicon), mouse anti-human chondroitin sulfate proteoglycan (CSPG, 1∶100, Chemicon), mouse anti-human fibronectin (1∶100, Chemicon), rabbit anti-glial fibrillary acidic protein (GFAP, 1∶250, DAKO), mouse anti-human low affinity neurotrophin receptor p75 (p75 NTR , 1∶750, USBiological), and mouse anti-β Tubulin III (anti-Tubulin, 1∶75, Sigma).

Techniques: Labeling

Journal: PLoS ONE

Article Title: Fibroblasts Isolated from Human Middle Turbinate Mucosa Cause Neural Progenitor Cells to Differentiate into Glial Lineage Cells

doi: 10.1371/journal.pone.0076926

Figure Lengend Snippet: The percentage of p75 NTR and GFAP positive cells in the total cells (A) from middle turbinates of 7 patients. Representative photographs of middle turbinate tissue: patient 1 (B) and patient 6 (C). In C, bone was separated from lamina propria. Note that the size of samples from two patients was similar, but the yields of OECs were very different.

Article Snippet: The following primary antibodies were used: mouse anti-human Thy1.1 (1∶1200, Serotec), rabbit anti-human basic fibroblast growth factor (FGF2, 1∶100, Santa Cruz Biotechnology), mouse anti-human Nestin (1∶200, Chemicon), mouse anti-human chondroitin sulfate proteoglycan (CSPG, 1∶100, Chemicon), mouse anti-human fibronectin (1∶100, Chemicon), rabbit anti-glial fibrillary acidic protein (GFAP, 1∶250, DAKO), mouse anti-human low affinity neurotrophin receptor p75 (p75 NTR , 1∶750, USBiological), and mouse anti-β Tubulin III (anti-Tubulin, 1∶75, Sigma).

Techniques:

Journal: Journal of Neurophysiology

Article Title: Adenosine inhibits glutamatergic input to basal forebrain cholinergic neurons

doi: 10.1152/jn.00528.2011

Figure Lengend Snippet: Cell counting in BF nuclei of neurons labeled by Cy3-p75 NTR -IgG and positive for ChAT immunoreactivity

Article Snippet: More recently, a new polyclonal fluorescent antibody against murine p75 NTR (Cy3-p75 NTR -IgG; Advanced Targeting Systems) has become available.

Techniques: Cell Counting, Labeling

Journal: Journal of Neurophysiology

Article Title: Adenosine inhibits glutamatergic input to basal forebrain cholinergic neurons

doi: 10.1152/jn.00528.2011

Figure Lengend Snippet: Cy3-coupled antibodies raised against mouse p75 neurotrophin receptors (Cy3-p75NTR-IgGs) specifically label cholinergic neurons in the basal forebrain (BF). Photographs show neurons of the horizontal limb of the diagonal band (hDB), magnocellular preoptic nucleus (MCPO), and substantia innominata (SI) nuclei. p75NTR-IgG label is shown in red and choline acetyltransferase (ChAT) immunoreactivity in green. A: ChAT immunostaining in the hDB shown at low magnification (left; scale bar = 100 μm). ChAT-positive neurons and p75NTR-IgG-positive neurons (boxed area) are shown at higher magnification at middle and right, respectively (scale bars = 25 μm). B: ChAT immunostaining in the MCPO and SI at the level of the crossing fibers of the anterior commissure is shown at low magnification (top row, left; scale bar = 200 μm). ChAT-positive neurons and p75NTR-IgG-positive neurons of the MCPO (bottom boxed area) and of the SI (top boxed area) are displayed at higher magnification at top row, middle and right, for MCPO and at bottom row, left and middle, for SI (scale bars = 25 μm).

Article Snippet: More recently, a new polyclonal fluorescent antibody against murine p75 NTR (Cy3-p75 NTR -IgG; Advanced Targeting Systems) has become available.

Techniques: Immunostaining