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Image Search Results
Journal: Proceedings of the National Academy of Sciences of the United States of America
Article Title: BRCT-domain protein BRIT1 influences class switch recombination.
doi: 10.1073/pnas.1708211114
Figure Lengend Snippet: Fig. 5. BRIT1 and MDC1 could both contribute to the ATM–γ-H2AX axis of DSB resolution during CSR. (A) Representative flow cytometry of CSR to IgG1 in splenic B cells derived from Mb1-Cre control, BRIT1 KO, and AID KO mice treated with ATMi or with DMSO vehicle control. (B) Quantification of CSR to IgG1 (n = 3; **P < 0.005, *P < 0.05). (C) Immunoblot analysis of whole- cell lysates from control, BRIT1 KO, or AID KO cells treated with ATMi or DMSO. (D) Immunoblot analysis of whole-cell lysates from control or BRIT1 KO cells transduced with two different MDC1 shRNAs (sh1 and sh2) or scrambled control. (E) Representative flow cytometry of CSR to IgG1 in splenic B cells of indicated genotypes expressing MDC1 knockdown shRNAs sh1 or sh2. (F) Quantification of CSR to IgG1 (n = 3; **P < 0.005, *P < 0.05).
Article Snippet: The shRNAs (sh3 and sh4) against
Techniques: Flow Cytometry, Derivative Assay, Control, Western Blot, Transduction, Expressing, Knockdown