Journal: bioRxiv

Article Title: Rat hepatitis E virus (HEV) cross-species infection and transmission in pigs

doi: 10.1101/2023.07.06.547957

Figure Lengend Snippet: (A) Workflow of HEV capping and transfection of target cells. LMTK, A549, Huh7, ST cell lines were transfected with in vitro transcribed capped HEV RNA (rat HEV). (B) Flow cytometry quantification of LMTK, A549, Huh7, ST cells transfected with capped RNA transcripts of rat HEV; Huh7 transfected with cell culture adaptive Kernow-C1 genotype 3 P6 strain and non-cell culture adaptive genotype 1 Sar55 and Kernow-C1 genotype 3 P1 strain. Sar55, P6 and P1 belong to Paslahepevirus is used as the control to determine the replication ability of rat HEV. The assay was performed in the cells harvested on day 5 post transfection. Samples were fixed in methanol and probed with rabbit anti-ORF2 followed by goat anti-rabbit alexa fluor 594 antibodies. Each bar (mean ± SD) represents separate transfections stained in parallel and displays the mean of two independent biological experiments with three replicates per sample. (C) Immunofluorescence detection of HEV ORF2 antigen in methanol fixed LMTK, A549, Huh7, ST cells after 5 days post transfection. Cells are stained with goat anti-rabbit IgG H&L combined with anti-rabbit Alexa fluor 594 (red), and 4’ s, 6-diamidino-2-phenylindole (DAPI) (blue). (D) RT-qPCR data from the supernatants collected from day 5 of replication assay. (E) RT-qPCR data from the cell lysates collected from day 5 of replication assay.

Article Snippet: LMTK (isolated from mouse subcutaneous tissue, ATCC:CCL-1.3), A549 (isolated from human lung carcinoma, ATCC:CRM-CCL-185), Huh7 (isolated from human liver carcinoma), ST (isolated from swine testicular, ATCC:CRL-1746) and Huh7 (human hepatoma cells) S10-3 subclone ( , ) were used for the studies.

Techniques: Transfection, In Vitro, Flow Cytometry, Cell Culture, Control, Staining, Immunofluorescence, Quantitative RT-PCR

Journal: Frontiers in Oncology

Article Title: Knockdown of LMTK3 in the Endometrioid Adenocarcinoma Cell Line Ishikawa: Inhibition of Growth and Estrogen Receptor α

doi: 10.3389/fonc.2021.692282

Figure Lengend Snippet: The cytoplastic expression of LMTK3 with significant difference between EEC tumor and para-tumor groups of tissue microarray. (A) Low and high staining of LMTK3 in endometrioid adenocarcinoma tissues; scale bar: 50 µM. (B) Scatter plot of cytoplastic LMTK3 expression with significant difference between EEC tumor and para-tumor groups, * P < 0.05.

Article Snippet: The following antibodies were used: ERα rabbit monoclonal (#8644, Cell Signaling) and LMTK3 mouse monoclonal (#M06A, Abnova and #110516, Abcam).

Techniques: Expressing, Microarray, Staining

Journal: Frontiers in Oncology

Article Title: Knockdown of LMTK3 in the Endometrioid Adenocarcinoma Cell Line Ishikawa: Inhibition of Growth and Estrogen Receptor α

doi: 10.3389/fonc.2021.692282

Figure Lengend Snippet: Effects of LMTK3 knockdown on cell viability in Ishikawa cells by CCK-8 (n=3, ** P < 0.01, *** P < 0.001).

Article Snippet: The following antibodies were used: ERα rabbit monoclonal (#8644, Cell Signaling) and LMTK3 mouse monoclonal (#M06A, Abnova and #110516, Abcam).

Techniques: Knockdown, CCK-8 Assay

Journal: Frontiers in Oncology

Article Title: Knockdown of LMTK3 in the Endometrioid Adenocarcinoma Cell Line Ishikawa: Inhibition of Growth and Estrogen Receptor α

doi: 10.3389/fonc.2021.692282

Figure Lengend Snippet: Effects of LMTK3 knockdown on cell cycle arrest in Ishikawa cells by flow cytometry. (A) Representative diagrams of LMTK3 knockdown on cell cycle arrest in Ishikawa cells. (B) Statistical histogram of LMTK3 knockdown on cell cycle arrest in Ishikawa (n=3, * P < 0.05, *** P < 0.001). ns represents no statistical significance).

Article Snippet: The following antibodies were used: ERα rabbit monoclonal (#8644, Cell Signaling) and LMTK3 mouse monoclonal (#M06A, Abnova and #110516, Abcam).

Techniques: Knockdown, Flow Cytometry

Journal: Frontiers in Oncology

Article Title: Knockdown of LMTK3 in the Endometrioid Adenocarcinoma Cell Line Ishikawa: Inhibition of Growth and Estrogen Receptor α

doi: 10.3389/fonc.2021.692282

Figure Lengend Snippet: Effects of LMTK3 knockdown on apoptosis in Ishikawa cells by flow cytometry. (A) Representative diagrams of LMTK3 knockdown on apoptosis in Ishikawa cells. (B) Statistical histogram of LMTK3 knockdown on apoptosis in Ishikawa (n=3, ** P < 0.01).

Article Snippet: The following antibodies were used: ERα rabbit monoclonal (#8644, Cell Signaling) and LMTK3 mouse monoclonal (#M06A, Abnova and #110516, Abcam).

Techniques: Knockdown, Flow Cytometry

Journal: Frontiers in Oncology

Article Title: Knockdown of LMTK3 in the Endometrioid Adenocarcinoma Cell Line Ishikawa: Inhibition of Growth and Estrogen Receptor α

doi: 10.3389/fonc.2021.692282

Figure Lengend Snippet: A positive correlation between the mRNA expression of LMTK3 and ESR1 .

Article Snippet: The following antibodies were used: ERα rabbit monoclonal (#8644, Cell Signaling) and LMTK3 mouse monoclonal (#M06A, Abnova and #110516, Abcam).

Techniques: Expressing

Journal: Frontiers in Oncology

Article Title: Knockdown of LMTK3 in the Endometrioid Adenocarcinoma Cell Line Ishikawa: Inhibition of Growth and Estrogen Receptor α

doi: 10.3389/fonc.2021.692282

Figure Lengend Snippet: Knockdown of LMTK3 reduces the mRNA and protein levels of ERα. (A, B) Effects of LMTK3 knockdown on the mRNA level of ERα (n=3, ** P < 0.01, *** P < 0.001), ns represents no statistical significance). (C) Representative image of LMTK3 knockdown on the protein level of ERα. (D, E) Effects of LMTK3 knockdown on the protein level of ERα (n=3, *** P < 0.001). siControl represents nontargeting siRNA. Mock represents nontreatment. ns represents no statistical significance).

Article Snippet: The following antibodies were used: ERα rabbit monoclonal (#8644, Cell Signaling) and LMTK3 mouse monoclonal (#M06A, Abnova and #110516, Abcam).

Techniques: Knockdown

Journal:

Article Title: Toward the development of multi-epitope p53 cancer vaccines: an in vitro assessment of CD8 + T-cell responses to HLA class I-restricted wild-type sequence p53 peptides

doi: 10.1016/j.clim.2007.05.015

Figure Lengend Snippet: Characteristics of the HLA-A2 and/or A24-positive patients with SCCHN tested in this study

Article Snippet: All subjects were HLA-A2 + and/or HLA-A24 + , as determined by flow cytometry using HLA-A2-specific antibodies (MA2.1 and BB7.2) and an HLA-A24-specific antibody (anti-HLA-A23, A24 mAb, IgG2b) purchased from American Type Culture Collection (ATCC, Rockville, MD) and One Lambda, Inc. (Canoga Park, CA), respectively. table ft1 table-wrap mode="anchored" t5 caption a7 patient age/sex HLA typing A2/A24 tumor site stage histology disease status at time of evaluation a SCCHN-1 53M +/− Oral cavity III SCC (mod) Local Rec.

Techniques: Immunopeptidomics

Journal:

Article Title: Toward the development of multi-epitope p53 cancer vaccines: an in vitro assessment of CD8 + T-cell responses to HLA class I-restricted wild-type sequence p53 peptides

doi: 10.1016/j.clim.2007.05.015

Figure Lengend Snippet: HLA-A2 and -A24 restricted wt-p53 derived peptides used in this study

Article Snippet: All subjects were HLA-A2 + and/or HLA-A24 + , as determined by flow cytometry using HLA-A2-specific antibodies (MA2.1 and BB7.2) and an HLA-A24-specific antibody (anti-HLA-A23, A24 mAb, IgG2b) purchased from American Type Culture Collection (ATCC, Rockville, MD) and One Lambda, Inc. (Canoga Park, CA), respectively. table ft1 table-wrap mode="anchored" t5 caption a7 patient age/sex HLA typing A2/A24 tumor site stage histology disease status at time of evaluation a SCCHN-1 53M +/− Oral cavity III SCC (mod) Local Rec.

Techniques: Derivative Assay, Sequencing

Journal:

Article Title: Toward the development of multi-epitope p53 cancer vaccines: an in vitro assessment of CD8 + T-cell responses to HLA class I-restricted wild-type sequence p53 peptides

doi: 10.1016/j.clim.2007.05.015

Figure Lengend Snippet: Healthy donor CD8+ T-cell responses to wt p53-derived peptides

Article Snippet: All subjects were HLA-A2 + and/or HLA-A24 + , as determined by flow cytometry using HLA-A2-specific antibodies (MA2.1 and BB7.2) and an HLA-A24-specific antibody (anti-HLA-A23, A24 mAb, IgG2b) purchased from American Type Culture Collection (ATCC, Rockville, MD) and One Lambda, Inc. (Canoga Park, CA), respectively. table ft1 table-wrap mode="anchored" t5 caption a7 patient age/sex HLA typing A2/A24 tumor site stage histology disease status at time of evaluation a SCCHN-1 53M +/− Oral cavity III SCC (mod) Local Rec.

Techniques:

Journal:

Article Title: Toward the development of multi-epitope p53 cancer vaccines: an in vitro assessment of CD8 + T-cell responses to HLA class I-restricted wild-type sequence p53 peptides

doi: 10.1016/j.clim.2007.05.015

Figure Lengend Snippet: CD8+ T-cell responses to wt-p53 derived peptides in HLA-A2-positive patients with SCCHN

Article Snippet: All subjects were HLA-A2 + and/or HLA-A24 + , as determined by flow cytometry using HLA-A2-specific antibodies (MA2.1 and BB7.2) and an HLA-A24-specific antibody (anti-HLA-A23, A24 mAb, IgG2b) purchased from American Type Culture Collection (ATCC, Rockville, MD) and One Lambda, Inc. (Canoga Park, CA), respectively. table ft1 table-wrap mode="anchored" t5 caption a7 patient age/sex HLA typing A2/A24 tumor site stage histology disease status at time of evaluation a SCCHN-1 53M +/− Oral cavity III SCC (mod) Local Rec.

Techniques: Derivative Assay

Journal:

Article Title: Toward the development of multi-epitope p53 cancer vaccines: an in vitro assessment of CD8 + T-cell responses to HLA class I-restricted wild-type sequence p53 peptides

doi: 10.1016/j.clim.2007.05.015

Figure Lengend Snippet: Positive (+) and negative (-) responsiveness to HLA class I-restricted wt p53 peptides of CD8+ T cells present in PBMC obtained from HLA-A2+ and/or HLA-A24+ subjects with SCCHN following 1x IVS are plotted against (A) Th1/Th2 ratio, (B) Tc1/Tc2 ratio and (c) % Treg of subject's PBMC. As indicated in panel (A) positive wt p53 peptide-specific CD8+ T cell responses were only significant relative to Tc1/Tc2 ratios of subject's PBMC.

Article Snippet: All subjects were HLA-A2 + and/or HLA-A24 + , as determined by flow cytometry using HLA-A2-specific antibodies (MA2.1 and BB7.2) and an HLA-A24-specific antibody (anti-HLA-A23, A24 mAb, IgG2b) purchased from American Type Culture Collection (ATCC, Rockville, MD) and One Lambda, Inc. (Canoga Park, CA), respectively. table ft1 table-wrap mode="anchored" t5 caption a7 patient age/sex HLA typing A2/A24 tumor site stage histology disease status at time of evaluation a SCCHN-1 53M +/− Oral cavity III SCC (mod) Local Rec.

Techniques: