mouse il-6 elisa Search Results


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CD74 expression and antigen presentation activity in Faslpr mice. A. Immunoblot detected CD74 expression, both the p41 full-length products and p10 processed fragments, in kidney extracts from 24-week-old WT and Faslpr mice. B. Immunostaining to detect CD74 (p41) expression in kidneys from 24-week-old WT mice. Scale: 50 μm. C. Immunostaining detected CD74 (p41) expression in kidneys from 24-week-old Faslpr mice. Scale: 200 μm and 50 μm, inset scale: 50 μm. D. CD74 (p41) expression in kidney TECs before and after stimulation with <t>IL6</t> and IFN-γ. E. CD4+ T cell culture medium IL2 levels after activation with TECs from 24-week-old WT and Faslpr mice in the presence or absence of autoantigens as indicated. F. Mixed lymphocyte reactions of presenter splenocytes from 24-week-old FaslprCd74+/+ and FaslprCd74−/− mice and responder cells from bm12 mice. G. Autoantigen histone presentation assay. ELISA determined IL2 levels in splenocytes from 24-week-old FaslprCd74+/+ and FaslprCd74−/− mice treated with and without histone. Data in E–G are representative of three independent experiments.
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R&D Systems directions rd systems cat m6000b
CD74 expression and antigen presentation activity in Faslpr mice. A. Immunoblot detected CD74 expression, both the p41 full-length products and p10 processed fragments, in kidney extracts from 24-week-old WT and Faslpr mice. B. Immunostaining to detect CD74 (p41) expression in kidneys from 24-week-old WT mice. Scale: 50 μm. C. Immunostaining detected CD74 (p41) expression in kidneys from 24-week-old Faslpr mice. Scale: 200 μm and 50 μm, inset scale: 50 μm. D. CD74 (p41) expression in kidney TECs before and after stimulation with <t>IL6</t> and IFN-γ. E. CD4+ T cell culture medium IL2 levels after activation with TECs from 24-week-old WT and Faslpr mice in the presence or absence of autoantigens as indicated. F. Mixed lymphocyte reactions of presenter splenocytes from 24-week-old FaslprCd74+/+ and FaslprCd74−/− mice and responder cells from bm12 mice. G. Autoantigen histone presentation assay. ELISA determined IL2 levels in splenocytes from 24-week-old FaslprCd74+/+ and FaslprCd74−/− mice treated with and without histone. Data in E–G are representative of three independent experiments.
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R&D Systems mouse il6 duoset elisa
CD74 expression and antigen presentation activity in Faslpr mice. A. Immunoblot detected CD74 expression, both the p41 full-length products and p10 processed fragments, in kidney extracts from 24-week-old WT and Faslpr mice. B. Immunostaining to detect CD74 (p41) expression in kidneys from 24-week-old WT mice. Scale: 50 μm. C. Immunostaining detected CD74 (p41) expression in kidneys from 24-week-old Faslpr mice. Scale: 200 μm and 50 μm, inset scale: 50 μm. D. CD74 (p41) expression in kidney TECs before and after stimulation with <t>IL6</t> and IFN-γ. E. CD4+ T cell culture medium IL2 levels after activation with TECs from 24-week-old WT and Faslpr mice in the presence or absence of autoantigens as indicated. F. Mixed lymphocyte reactions of presenter splenocytes from 24-week-old FaslprCd74+/+ and FaslprCd74−/− mice and responder cells from bm12 mice. G. Autoantigen histone presentation assay. ELISA determined IL2 levels in splenocytes from 24-week-old FaslprCd74+/+ and FaslprCd74−/− mice treated with and without histone. Data in E–G are representative of three independent experiments.
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CD74 expression and antigen presentation activity in Faslpr mice. A. Immunoblot detected CD74 expression, both the p41 full-length products and p10 processed fragments, in kidney extracts from 24-week-old WT and Faslpr mice. B. Immunostaining to detect CD74 (p41) expression in kidneys from 24-week-old WT mice. Scale: 50 μm. C. Immunostaining detected CD74 (p41) expression in kidneys from 24-week-old Faslpr mice. Scale: 200 μm and 50 μm, inset scale: 50 μm. D. CD74 (p41) expression in kidney TECs before and after stimulation with <t>IL6</t> and IFN-γ. E. CD4+ T cell culture medium IL2 levels after activation with TECs from 24-week-old WT and Faslpr mice in the presence or absence of autoantigens as indicated. F. Mixed lymphocyte reactions of presenter splenocytes from 24-week-old FaslprCd74+/+ and FaslprCd74−/− mice and responder cells from bm12 mice. G. Autoantigen histone presentation assay. ELISA determined IL2 levels in splenocytes from 24-week-old FaslprCd74+/+ and FaslprCd74−/− mice treated with and without histone. Data in E–G are representative of three independent experiments.
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CD74 expression and antigen presentation activity in Faslpr mice. A. Immunoblot detected CD74 expression, both the p41 full-length products and p10 processed fragments, in kidney extracts from 24-week-old WT and Faslpr mice. B. Immunostaining to detect CD74 (p41) expression in kidneys from 24-week-old WT mice. Scale: 50 μm. C. Immunostaining detected CD74 (p41) expression in kidneys from 24-week-old Faslpr mice. Scale: 200 μm and 50 μm, inset scale: 50 μm. D. CD74 (p41) expression in kidney TECs before and after stimulation with <t>IL6</t> and IFN-γ. E. CD4+ T cell culture medium IL2 levels after activation with TECs from 24-week-old WT and Faslpr mice in the presence or absence of autoantigens as indicated. F. Mixed lymphocyte reactions of presenter splenocytes from 24-week-old FaslprCd74+/+ and FaslprCd74−/− mice and responder cells from bm12 mice. G. Autoantigen histone presentation assay. ELISA determined IL2 levels in splenocytes from 24-week-old FaslprCd74+/+ and FaslprCd74−/− mice treated with and without histone. Data in E–G are representative of three independent experiments.
Il 8, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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CD74 expression and antigen presentation activity in Faslpr mice. A. Immunoblot detected CD74 expression, both the p41 full-length products and p10 processed fragments, in kidney extracts from 24-week-old WT and Faslpr mice. B. Immunostaining to detect CD74 (p41) expression in kidneys from 24-week-old WT mice. Scale: 50 μm. C. Immunostaining detected CD74 (p41) expression in kidneys from 24-week-old Faslpr mice. Scale: 200 μm and 50 μm, inset scale: 50 μm. D. CD74 (p41) expression in kidney TECs before and after stimulation with <t>IL6</t> and IFN-γ. E. CD4+ T cell culture medium IL2 levels after activation with TECs from 24-week-old WT and Faslpr mice in the presence or absence of autoantigens as indicated. F. Mixed lymphocyte reactions of presenter splenocytes from 24-week-old FaslprCd74+/+ and FaslprCd74−/− mice and responder cells from bm12 mice. G. Autoantigen histone presentation assay. ELISA determined IL2 levels in splenocytes from 24-week-old FaslprCd74+/+ and FaslprCd74−/− mice treated with and without histone. Data in E–G are representative of three independent experiments.
Ek206hs, supplied by Multi Sciences (Lianke) Biotech Co Ltd, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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CD74 expression and antigen presentation activity in Faslpr mice. A. Immunoblot detected CD74 expression, both the p41 full-length products and p10 processed fragments, in kidney extracts from 24-week-old WT and Faslpr mice. B. Immunostaining to detect CD74 (p41) expression in kidneys from 24-week-old WT mice. Scale: 50 μm. C. Immunostaining detected CD74 (p41) expression in kidneys from 24-week-old Faslpr mice. Scale: 200 μm and 50 μm, inset scale: 50 μm. D. CD74 (p41) expression in kidney TECs before and after stimulation with <t>IL6</t> and IFN-γ. E. CD4+ T cell culture medium IL2 levels after activation with TECs from 24-week-old WT and Faslpr mice in the presence or absence of autoantigens as indicated. F. Mixed lymphocyte reactions of presenter splenocytes from 24-week-old FaslprCd74+/+ and FaslprCd74−/− mice and responder cells from bm12 mice. G. Autoantigen histone presentation assay. ELISA determined IL2 levels in splenocytes from 24-week-old FaslprCd74+/+ and FaslprCd74−/− mice treated with and without histone. Data in E–G are representative of three independent experiments.
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CD74 expression and antigen presentation activity in Faslpr mice. A. Immunoblot detected CD74 expression, both the p41 full-length products and p10 processed fragments, in kidney extracts from 24-week-old WT and Faslpr mice. B. Immunostaining to detect CD74 (p41) expression in kidneys from 24-week-old WT mice. Scale: 50 μm. C. Immunostaining detected CD74 (p41) expression in kidneys from 24-week-old Faslpr mice. Scale: 200 μm and 50 μm, inset scale: 50 μm. D. CD74 (p41) expression in kidney TECs before and after stimulation with IL6 and IFN-γ. E. CD4+ T cell culture medium IL2 levels after activation with TECs from 24-week-old WT and Faslpr mice in the presence or absence of autoantigens as indicated. F. Mixed lymphocyte reactions of presenter splenocytes from 24-week-old FaslprCd74+/+ and FaslprCd74−/− mice and responder cells from bm12 mice. G. Autoantigen histone presentation assay. ELISA determined IL2 levels in splenocytes from 24-week-old FaslprCd74+/+ and FaslprCd74−/− mice treated with and without histone. Data in E–G are representative of three independent experiments.

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: CD74 deficiency mitigates systemic lupus erythematosus-like autoimmunity and pathological findings in mice

doi: 10.4049/jimmunol.1600028

Figure Lengend Snippet: CD74 expression and antigen presentation activity in Faslpr mice. A. Immunoblot detected CD74 expression, both the p41 full-length products and p10 processed fragments, in kidney extracts from 24-week-old WT and Faslpr mice. B. Immunostaining to detect CD74 (p41) expression in kidneys from 24-week-old WT mice. Scale: 50 μm. C. Immunostaining detected CD74 (p41) expression in kidneys from 24-week-old Faslpr mice. Scale: 200 μm and 50 μm, inset scale: 50 μm. D. CD74 (p41) expression in kidney TECs before and after stimulation with IL6 and IFN-γ. E. CD4+ T cell culture medium IL2 levels after activation with TECs from 24-week-old WT and Faslpr mice in the presence or absence of autoantigens as indicated. F. Mixed lymphocyte reactions of presenter splenocytes from 24-week-old FaslprCd74+/+ and FaslprCd74−/− mice and responder cells from bm12 mice. G. Autoantigen histone presentation assay. ELISA determined IL2 levels in splenocytes from 24-week-old FaslprCd74+/+ and FaslprCd74−/− mice treated with and without histone. Data in E–G are representative of three independent experiments.

Article Snippet: The ELISA kits used in this study include: mouse IL6 DuoSet (DY406, R&D Systems, Minneapolis, MN), mouse IL2 DuoSet (DY402, R&D Systems), mouse TGF-β ELISA ready-SET-Go (88-8350, eBioscience, San Diego, CA), mouse IL17A Platinum ELISA (BMS6001, eBioscience), mouse IgG ELISA Quantitation Set (E90-131, Bethyl Laboratories, Inc., Montgomery, TX), and mouse IgG1 ELISA Quantitation Set (E90-105, Bethyl Laboratories, Inc).

Techniques: Expressing, Immunopeptidomics, Activity Assay, Western Blot, Immunostaining, Cell Culture, Activation Assay, Enzyme-linked Immunosorbent Assay

CD74 deficiency mitigates SLE-like pathology in SLE-prone Faslpr mice. A. Kidney PAS staining and glomerulus and tubulointerstitial scores; B. Kidney IgG and C3 immunofluorescent staining and glomerulus scores. C. Kidney Mac-2, MCP-1, and MHC-II immunostaining. D. ELISA determined serum IL6, IL17A, IL2, and TGF-β levels from 24-week-old FaslprCd74+/+ and FaslprCd74−/− mice. The number of mice per group is indicated in each parenthesis. Representative graphs for A–C are shown to the left of each panel. Scale: 50 μm.

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: CD74 deficiency mitigates systemic lupus erythematosus-like autoimmunity and pathological findings in mice

doi: 10.4049/jimmunol.1600028

Figure Lengend Snippet: CD74 deficiency mitigates SLE-like pathology in SLE-prone Faslpr mice. A. Kidney PAS staining and glomerulus and tubulointerstitial scores; B. Kidney IgG and C3 immunofluorescent staining and glomerulus scores. C. Kidney Mac-2, MCP-1, and MHC-II immunostaining. D. ELISA determined serum IL6, IL17A, IL2, and TGF-β levels from 24-week-old FaslprCd74+/+ and FaslprCd74−/− mice. The number of mice per group is indicated in each parenthesis. Representative graphs for A–C are shown to the left of each panel. Scale: 50 μm.

Article Snippet: The ELISA kits used in this study include: mouse IL6 DuoSet (DY406, R&D Systems, Minneapolis, MN), mouse IL2 DuoSet (DY402, R&D Systems), mouse TGF-β ELISA ready-SET-Go (88-8350, eBioscience, San Diego, CA), mouse IL17A Platinum ELISA (BMS6001, eBioscience), mouse IgG ELISA Quantitation Set (E90-131, Bethyl Laboratories, Inc., Montgomery, TX), and mouse IgG1 ELISA Quantitation Set (E90-105, Bethyl Laboratories, Inc).

Techniques: Staining, Immunostaining, Enzyme-linked Immunosorbent Assay