Journal: American Journal of Respiratory Cell and Molecular Biology

Article Title: Both Hematopoietic-Derived and Non-Hematopoietic-Derived ?-Arrestin-2 Regulates Murine Allergic Airway Disease

doi: 10.1165/rcmb.2009-0198OC

Figure Lengend Snippet: Hematopoietic-derived β-arrestin−2 and OVA challenge. Bone marrow chimeric mice express β-arrestin−2 exclusively in hematopoietic cells (H+/S−), structural cells (H−/S+), both (H+/S+), or neither (H−/S−). Chimeras were immunized and challenged with OVA. Hematopoietic-derived β-arrestin−2 is required for airspace recruitment of (A) eosinophils and (B) lymphocytes. (C, D) Hematopoietic-derived β-arrestin−2 is required for inflammatory cell recruitment into the airway subepithelial space. (E, F) Mucus staining is significantly elevated in the presence of hematopoietic β-arrestin−2 (PAS, periodic acid-Schiff). Lavage eotaxin is increased in all OVA-challenged animals compared with sham immunization (G), whereas tissue IL-13 is only elevated in association with hematopoietic β-arrestin−2 (H). Histology is representative of 4–11 mice in each group (original magnification, ×40) (*P < 0.05).

Article Snippet: All sample concentrations were adjusted so that equal amounts of protein were used in a Mouse IL-13 Quantikine ELISA kit (R&D Systems, Inc.), according to the manufacturer's instructions.

Techniques: Derivative Assay, Staining

Journal: American Journal of Respiratory Cell and Molecular Biology

Article Title: Both Hematopoietic-Derived and Non-Hematopoietic-Derived ?-Arrestin-2 Regulates Murine Allergic Airway Disease

doi: 10.1165/rcmb.2009-0198OC

Figure Lengend Snippet: Hematopoietic-derived β-arrestin−2 and IL-13 challenge. Bone marrow chimeric mice express β-arrestin−2 exclusively in hematopoietic cells (H+/S−), structural cells (H−/S+), both (H+/S+), or neither (H−/S−). Chimeras were challenged with recombinant murine IL-13. β-arr2, β-arrestin−2; WT, wild-type; PAS, periodic acid-Schiff. (A) Hematopoietic-derived β-arrestin−2 is required for airspace recruitment of eosinophils. (B) No difference in airspace lymphocytes is evident. Ubiquitous expression of β-arrestin−2 is required for (C, D) inflammatory cell recruitment into the airway subepithelial space and (E, F) epithelial mucus staining. (G) Eotaxin is increased in all IL-13 challenged animals compared with sham challenge. Histology is representative of 4–11 mice in each group (original magnification, ×40) (*P < 0.05).

Article Snippet: All sample concentrations were adjusted so that equal amounts of protein were used in a Mouse IL-13 Quantikine ELISA kit (R&D Systems, Inc.), according to the manufacturer's instructions.

Techniques: Derivative Assay, Recombinant, Expressing, Staining

Journal: American Journal of Respiratory Cell and Molecular Biology

Article Title: Both Hematopoietic-Derived and Non-Hematopoietic-Derived ?-Arrestin-2 Regulates Murine Allergic Airway Disease

doi: 10.1165/rcmb.2009-0198OC

Figure Lengend Snippet: Effect of T lymphocytes on response to IL-13. The adoptive transfer of wild-type or β-arrestin−2–deficient T lymphocytes into recombinase activating gene-1 knockout (RAG-KO) mice had no significant effect on the IL-13–induced (A) percentage of eosinophils in lung lavage or (B) airway hyperresponsiveness. MCh, methacholine.

Article Snippet: All sample concentrations were adjusted so that equal amounts of protein were used in a Mouse IL-13 Quantikine ELISA kit (R&D Systems, Inc.), according to the manufacturer's instructions.

Techniques: Adoptive Transfer Assay, Knock-Out

Journal: American Journal of Respiratory Cell and Molecular Biology

Article Title: Both Hematopoietic-Derived and Non-Hematopoietic-Derived ?-Arrestin-2 Regulates Murine Allergic Airway Disease

doi: 10.1165/rcmb.2009-0198OC

Figure Lengend Snippet: Nonhematopoietic β-arrestin−2 and airway hyperresponsiveness. Airway responsiveness to methacholine (MCh) is dependent on non–hematopoietic-derived β-arrestin−2 in OVA-sensitized and OVA-challenged mice (A), but is independent of β-arrestin−2 in IL-13-challenged mice (B). (*P < 0.05)

Article Snippet: All sample concentrations were adjusted so that equal amounts of protein were used in a Mouse IL-13 Quantikine ELISA kit (R&D Systems, Inc.), according to the manufacturer's instructions.

Techniques: Derivative Assay