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Image Search Results
Journal: Immunology
Article Title: Toll-like receptor agonists induce inflammation and cell death in a model of head and neck squamous cell carcinomas.
doi: 10.1111/j.1365-2567.2008.03041.x
Figure Lengend Snippet: Figure 3. Toll-like receptor (TLR) agonists induce up-regulation of intercellular adhesion molecule 1 (ICAM-1) in Detroit-562. The cells were stimulated with or without Pam3CSK4, poly(I:C), flagellin or tumour necrosis factor (TNF)-a (10 ng/ml) for 6 or 24 hr. Thereaf- ter, the cells were stained with monoclonal antibodies against ICAM- 1 and analysed by fluorescence-activated cell sorting (FACS). The mean fluorescence intensity (MFI) of the ICAM-1-positive cells was calculated. (a) ICAM-1 expression after stimulation for 24 hr. (b) Time-dependent up-regulation of ICAM-1 by poly(I:C) (C = control; n = 4; *P < 005; **P < 001; ***P < 0001).
Article Snippet: The following monoclonal antibodies (mAbs) were used for flow cytometry analysis:
Techniques: Staining, Bioprocessing, FACS, Expressing, Control
Journal: Immunology
Article Title: Toll-like receptor agonists induce inflammation and cell death in a model of head and neck squamous cell carcinomas.
doi: 10.1111/j.1365-2567.2008.03041.x
Figure Lengend Snippet: Figure 6. Responses induced by Toll-like receptor (TLR) ligands in human nasal epithelial cells (HNECs). (a) HNECs were analysed for expres- sion of TLR mRNA using real-time reverse transcription–polymerase chain reaction (RT-PCR). The values are expressed in relation to the house- keeping gene b-actin (n = 5). (b) The cells were cultured with or without Pam3CSK4, poly(I:C), flagellin or tumour necrosis factor (TNF)-a (10 ng/ml) for 24 hr. Thereafter, cells were stained with monoclonal antibodies against intercellular adhesion molecule 1 (ICAM-1) and analysed by fluorescence-activated cell sorting (FACS). The mean fluorescence intensity (MFI) of the ICAM-1-positive cells was calculated (n = 4). (c, d, e) After stimulation, the cell culture supernatants were analysed for (c) interleukin (IL)-1b, (d) IL-6 and (e) IL-8 production by enzyme-linked immunosorbent assay (ELISA) (C = control; n = 6; *P < 005; **P < 001).
Article Snippet: The following monoclonal antibodies (mAbs) were used for flow cytometry analysis:
Techniques: Reverse Transcription, Polymerase Chain Reaction, Reverse Transcription Polymerase Chain Reaction, Cell Culture, Staining, Bioprocessing, FACS, Enzyme-linked Immunosorbent Assay, Control