Journal: Kidney international

Article Title: Targeted reduction of advanced glycation improves renal function in obesity.

doi: 10.1038/ki.2011.57

Figure Lengend Snippet: Figure 2 | Murine renal and biochemical parameters at study completion. Groups of mice were followed for 16 weeks. (a) Urinary albumin excretion rate (AER) over 24 h measured by enzyme-linked immunosorbent assay (ELISA). (b) Creatinine clearance (CrCl) as determined by high-performance liquid chromatography (HPLC) following correction for body surface area. (c–e) N-Carboxymethyllysine (CML) analyzed by ELISA in plasma (c), dietary CML consumption over 24 h (d), and kidney cortex protein (e). (f) CML immunohistochemistry staining on paraffin-fixed kidney sections from (A) lean low advanced glycation end-product (AGE), (B) obese, (C) obese alagebrium (ALA), and (D) obese RAGE–/–. Obese (high AGE/high-fat diet), ALA (AGE-lowering therapy, alagebrium chloride 1 mg/kg/day), and RAGE–/– (RAGE deletion). Data for AER were logarithmically transformed as these were not normally distributed. Other data are presented as mean±s.d. *Po0.05 vs lean low AGE, **Po0.01 vs lean low AGE, ***Po0.001 vs lean low AGE, wPo0.05 vs obese.

Article Snippet: Albumin excretion rate was assessed using a mouse albumin ELISA kit according to the manufacturer’s instructions (Bethyl Laboratories, Montgomery, TX).

Techniques: Enzyme-linked Immunosorbent Assay, High Performance Liquid Chromatography, Clinical Proteomics, Immunohistochemistry, Staining, Transformation Assay

Journal: Nature communications

Article Title: Generation of colonic IgA-secreting cells in the caecal patch.

doi: 10.1038/ncomms4704

Figure Lengend Snippet: Figure 2 | Decreased IgA-secreting cells in large intestinal lamina propria of appendectomized germ-free mice after conventionalization. (a,b) Lamina propria lymphocytes were isolated from appendectomized or sham-operated germ-free(GF) mice at 4 weeks after conventionalization, and stained for surface IgA/B220 or intracellular IL-17/IFN-g/IL-10 in CD4 þ population. (a) Total numbers of IgA þ B220 cells as well as IL-17-, IFN-g- and IL-10-positive CD4 þ cells in the large (upper) and the small intestine (lower) are shown. Each symbol represents an individual mouse, and horizontal bars indicate the mean. (b) Representative dot plots for cells stained with anti-IgA and B220 of appendectomized and sham-operated mice. Numbers within plots indicate percentages of cells in respective areas. (c) Distribution of IgA þ cells in the small and large intestines. Frozen sections were stained with anti-IgA mAb (green) and DAPI (blue). Scale bars, 100 mm. (d) Faeces and sera were collected from appendectomized (n ¼ 12) or sham-operated mice (n ¼ 12) at 4 weeks after conventionalization and GF mice (n ¼ 12), and IgA levels of faeces and sera were determined by ELISA. Each symbol represents an individual mouse, and horizontal bars indicate the mean. Statistical analyses were performed with the Student’s t-test. *Po0.05. NS, not significant.

Article Snippet: IgA levels in the faeces, sera and culture supernatants were determined by a mouse IgA ELISA quantitation kit (Bethyl Laboratories).

Techniques: Isolation, Staining, Enzyme-linked Immunosorbent Assay

Journal: The Journal of nutritional biochemistry

Article Title: Resveratrol prevents the development of choroidal neovascularization by modulating AMP-activated protein kinase in macrophages and other cell types.

doi: 10.1016/j.jnutbio.2014.05.015

Figure Lengend Snippet: Fig. 3. RSV treatment reduced the levels of inflammatory and angiogenic molecules.Protein levels of VEGF (A), ICAM-1 (B) and MCP-1 (C) were increased 3 days after CNV induction. RSV pretreatment significantly suppressed these increases. n=8. **Pb.001, *Pb.01, †Pb.05.

Article Snippet: In the samples taken 3 days after the PC, VEGF, MCP-1 and ICAM-1 levels were determined with mouse VEGF, MCP-1 and ICAM-1 ELISA kits (R&D Systems, Minneapolis, MN, USA) as previously described [10].

Techniques:

Journal: The Journal of nutritional biochemistry

Article Title: Resveratrol prevents the development of choroidal neovascularization by modulating AMP-activated protein kinase in macrophages and other cell types.

doi: 10.1016/j.jnutbio.2014.05.015

Figure Lengend Snippet: Fig. 5. RSV inhibited NF-κB and inflammatory and angiogenic molecules in vitro. ARPE-19 cells (A–C), b-End3 microvascular endothelial cells (D–F) and RAW264.7 macrophages (G and H) were stimulated with TNF-α. RSV significantly suppressed the phosphorylation (activation) of NF-κB by TNF-α (A, D and G) and the subsequent induction of VEGF (B and H), MCP-1 (C and F) and ICAM-1 (E). All of the effects of RSV were attenuated by an AMPK inhibitor, compound C. n=8. **Pb.001, *Pb.01, †Pb.05.

Article Snippet: In the samples taken 3 days after the PC, VEGF, MCP-1 and ICAM-1 levels were determined with mouse VEGF, MCP-1 and ICAM-1 ELISA kits (R&D Systems, Minneapolis, MN, USA) as previously described [10].

Techniques: In Vitro, Phospho-proteomics, Activation Assay

Journal: The Journal of nutritional biochemistry

Article Title: Resveratrol prevents the development of choroidal neovascularization by modulating AMP-activated protein kinase in macrophages and other cell types.

doi: 10.1016/j.jnutbio.2014.05.015

Figure Lengend Snippet: Fig. 7. A model for the preventive effects of RSV on CNV.RSV treatment significantly suppresses CNV development by preserving AMPK activation, which inhibits macrophage recruitment as well as the induction of inflammatory processes, including NF-κB activation and the subsequent up-regulation of inflammatory and angiogenic molecules such as ICAM-1, MCP-1 and VEGF in multiple cell types. PC, laser PC for CNV induction; Vascular EC, vascular endothelial cells.

Article Snippet: In the samples taken 3 days after the PC, VEGF, MCP-1 and ICAM-1 levels were determined with mouse VEGF, MCP-1 and ICAM-1 ELISA kits (R&D Systems, Minneapolis, MN, USA) as previously described [10].

Techniques: Preserving, Activation Assay

Journal: PLoS ONE

Article Title: Type-I Interferon is Critical for FasL Expression on Lung Cells to Determine the Severity of Influenza

doi: 10.1371/journal.pone.0055321

Figure Lengend Snippet: B6 mice were intranasally infected with 10 5 (closed triangle) or 10 2 (open square) pfu/head of the PR/8 virus. At 0, 3 or 5 DPI, the BALF of these mice were isolated. The amount of IFN- ß or total protein contained in these samples was assessed by mouse IFN- ß specific ELISA or BCA protein assay, respectively. The amounts of IFN- ß were normalized by that of the total protein in each sample. “N.D.” means not detected.

Article Snippet: The amount of IFN-β was assessed by mouse IFN-beta ELISA kit (R&D systems, Abingdon, UK).

Techniques: Infection, Virus, Isolation, Enzyme-linked Immunosorbent Assay, Bicinchoninic Acid Protein Assay

Journal: EMBO Molecular Medicine

Article Title: An essential role for decorin in bladder cancer invasiveness

doi: 10.1002/emmm.201302655

Figure Lengend Snippet: Decorin is necessary and sufficient for increased invasiveness of MB49-I cells

Article Snippet: Decorin overexpression in MB49 cells was achieved using the pCMV6-Entry plasmid containing cDNA of mouse Decorin (NM_007833) purchased from Origene (Rockville, MD, USA).

Techniques: