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Image Search Results
Journal: Diabetes
Article Title: The WNT Inhibitor Dickkopf 1 and Bone Morphogenetic Protein 4 Rescue Adipogenesis in Hypertrophic Obesity in Humans
doi: 10.2337/db11-1419
Figure Lengend Snippet: DKK1 promotes differentiation of adipose tissue stromal cells from individuals with a low degree of differentiation. A : Stromal cells from subcutaneous adipose tissue were differentiated for 21 days with or without DKK1. Results are from two representative individuals. Accumulation of cellular triglyceride was detected with ORO ( upper panel ) or unstained cells ( lower panel ). B : Effect of DKK1 on differentiation is more pronounced in stromal cells from individuals with a low degree of differentiation. Differentiation (%) is related to the area of lipid-accumulating cells at day 21 in the cell culture well ( r 2 = 0.66, P < 0.01, n = 11). C : Differentiation of stromal cells is dependent on the presence of TZDs and cannot be replaced by DKK1. (A high-quality digital representation of this figure is available in the online issue.)
Article Snippet: Immunoblots were performed with the following antibodies; WIF1, sFRP1, and sFRP2 (sc-25520, sc-13939, and sc-13940 Santa Cruz, Biotechnology);
Techniques: Cell Culture
Journal: Diabetes
Article Title: The WNT Inhibitor Dickkopf 1 and Bone Morphogenetic Protein 4 Rescue Adipogenesis in Hypertrophic Obesity in Humans
doi: 10.2337/db11-1419
Figure Lengend Snippet: BMP4 induces commitment and differentiation of progenitor cells from adipose tissue stromal cells. Stromal cells were incubated for 5 days with 3 nmol/L (40 ng/mL) BMP4 before initiation of differentiation, and this was maintained during differentiation. Cells were also induced to differentiate with and without DKK1, as described. ORO/hematoxylin staining at day 14. Low magnification shows an overview of the additive effects of DKK1 and BMP4 on differentiation. (A high-quality digital representation of this figure is available in the online issue.)
Article Snippet: Immunoblots were performed with the following antibodies; WIF1, sFRP1, and sFRP2 (sc-25520, sc-13939, and sc-13940 Santa Cruz, Biotechnology);
Techniques: Incubation, Staining