Journal: Journal of Advanced Research

Article Title: Synergistic potentiation of the anti-metastatic effect of a Ginseng - Salvia miltiorrhiza herbal pair and its biological ingredients via the suppression of CD62E-dependent neutrophil infiltration and NETformation

doi: 10.1016/j.jare.2024.10.036

Figure Lengend Snippet: Rg1 reduced NETs in lung tissues. (A) Representative immunofluorescence images and quantification of H3cit and NE in the lungs (n = 3; scale bar: 50 μm). (B) Representative western blot images of H3cit and NE levels in the lungs and quantitative data ( n = 3). GAPDH was used as a loading control. (C-E) Serum levels of CXCL1, CXCL2, and G-CSF were detected via ELISA ( n = 10). (F-H) The mRNA expression levels of CXCL1, CXCL2 and G-CSF in lung tissues were determined by qRT–PCR ( n = 3). All the data are presented as mean ± SEM. # p < 0.05, ## p < 0.01, ### p < 0.001, #### p < 0.0001 vs. the control group. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 vs. the model group.

Article Snippet: The levels of mouse CXCL1, CXCL2, and G-CDF in the serum were measured via the Mouse CXCL1/KC ELISA Kit (EK296/2, Multi Sciences), Mouse CXCL2/MIP-2 ELISA Kit (EK2142, Multi Sciences), and Mouse G-CSF ELISA Kit (EK269/2, Multi Sciences) according to the manufacturers’ instructions.

Techniques: Immunofluorescence, Western Blot, Control, Enzyme-linked Immunosorbent Assay, Expressing, Quantitative RT-PCR

Journal: Acta neuropathologica communications

Article Title: Polarizing receptor activation dissociates fibroblast growth factor 2 mediated inhibition of myelination from its neuroprotective potential.

doi: 10.1186/s40478-019-0864-6

Figure Lengend Snippet: Fig. 4 FGFR1 supports induction of pro-myelinating and neuroprotective factors. Mouse myelinating cultures were treated with 100 ng/ml FGF2 or F2 V2 at DIV18 for 3 days and protein levels in the cell culture supernatant were analyzed by ELISA for CXCL1 (a) and TIMP1 (b) or by Proteom Profiler (c, Mouse Cytokine Array Panel a). a, b FGF2 treatment results in a significant increase in protein levels for both CXCL1 and MMP3 (p < 0.05), whereas F2 V2 only significantly increased the levels of CXCL1. Data are presented as means + SEM from 6 independent experiments; * p < 0.05. c TIMP1 levels are increased in both FGF2 and F2 V2 treated cultures; data are presented as technical duplicates from 2 independent experiments, pixel densities are represented as arbitrary units. d-f A monolayer of neurosphere-derived mouse astrocytes was treated with 100 ng/ml recombinant FGF2 for 3 days and concentrations of CXCL1 (d), MMP3 (e) or TIMP1 (f) were determined by ELISA (d, e) or Proteom Profiler (f, Mouse Cytokine Array Panel a). d, e Data are presented as means ± SEM from 4 independent experiments; * p < 0.05; ** p < 0.01 (paired t-test), f Data are presented as technical duplicates from 2 independent experiments, arbitrary units represent pixel densities. g FGFR1 expression co- localizes with GFAP+ astrocytes in active multiple sclerosis. h, i Myelinating CNS cultures were treated in the absence or presence of 100 ng/ml FGF2 for 10 days from DIV 18 onwards and either directly fixed and stained for MOG / SMI31 after a total of 28 days (28 DIV) or after 38 days in vitro (38 DIV); shown are mean + SEM from at least 4 independent experiments and p-values for paired t-test; * p < 0.05. j Representative confocal immunofluorescence images (63x magnification, Maximum Intensity Projection) of 38 DIV culture demonstrating withdrawal of FGF2 (18 DIV – 28 DIV) is followed by rapid remyelination resulting in the formation of myelinated internodes that are indistinguishable from those in untreated cultures. Scale bars represent 10 μm

Article Snippet: Protein concentrations of CXCL1 and MMP3 in the supernatants were measured using mouse CXCL1 or MMP3 Quantikine ELISA kits (both R&D Systems) according to the manufacturer’s instructions.

Techniques: Cell Culture, Enzyme-linked Immunosorbent Assay, Derivative Assay, Recombinant, Expressing, Staining, In Vitro, Immunofluorescence

Journal: Journal of clinical & cellular immunology

Article Title: The Anti-inflammatory Effect of Alpha-1 Antitrypsin in Rhinovirus-infected Human Airway Epithelial Cells

doi: 10.4172/2155-9899.1000475

Figure Lengend Snippet: Increased IL-8 production in cultured COPD brushed airway epithelial cells. Brushed airway epithelial cells from COPD patients (n=6) and normal subjects (n=6) were cultured under air-liquid interface (ALI) condition for 10 days. After 24 hours of air exposure, IL-8 was measured by ELISA. Data are expressed as means ± SEM.

Article Snippet: Protein levels of human IL-8, or mouse KC were determined by using a human IL-8 or a mouse KC DuoSet ELISA kit (R&D Systems, Minneapolis, MN) as per manufacturer’s instruction.

Techniques: Cell Culture, Enzyme-linked Immunosorbent Assay