Journal: Reproductive sciences (Thousand Oaks, Calif.)

Article Title: Conservative Hypomethylation of Mesenchymal Stem Cells and Their Secretome Restored the Follicular Development in Cisplatin-Induced Premature Ovarian Failure Mice.

doi: 10.1007/s43032-023-01389-4

Figure Lengend Snippet: Fig. 3 Phenotype characterization of partially hypomethylated MSCs. The expression of CD105, CD90, CD45, and CD34 was analyzed by flow cytometry for normally methylated cells (A), cells treated with 2.5 μM (B), or 5 μM (C) of 5-Aza-dC. No significant differences were

Article Snippet: Phycoerthrin (PE)-conjugated mouse monoclonal antibodies of CD90, and CD34 or FITC-conjugated monoclonal antibodies of CD105 and CD45 were from (Biotechne R&D System, MN, USA).

Techniques: Expressing, Flow Cytometry, Methylation

Journal: Brazilian Journal of Otorhinolaryngology

Article Title: Angiectatic nasal polyps with pleomorphism ‒ a diagnostic pitfall

doi: 10.1016/j.bjorl.2023.101281

Figure Lengend Snippet: Immunohistochemical Expression of Thirteen PANP cases.

Article Snippet: Markers are mouse anti-human antibodys, including ZM-0046 CD34 (OriGene, 1:100, clone 10C9), ZM-0069 CK (OriGene, 1:200, clone AE1/AE3), ZM-0260 Vim (OriGene, 1:200, clone UMAB159), ZA-0524 Calponin (EPI, 1:150, clone EP63), ZM-0166 Ki67 (OriGene, 1:200, clone UMAB107), ZM-0010 Bcl-2 (Leic, 1:100, clone Bcl-2/100/D5) and ZA-0647 STAT-6 (EPI, 1:150, clone EP325).

Techniques: Immunohistochemical staining, Expressing

Journal: Cells

Article Title: IMG-A1: A Novel Immortalized Granulosa Cell Line for Investigating FSH-Dependent Folliculogenesis and Ovarian Pathophysiology

doi: 10.3390/cells14241940

Figure Lengend Snippet: Basic Characterization of the IMG-A1 Granulosa Cell Line. ( a , b ) Phase-contrast micrographs of the IMG-A1 cell culture. ( c ) Staining for senescence-associated β-galactosidase activity in passage 18 cells. ( d – f ) Immunocytochemical staining for the proliferation marker Ki67, showing a phase-contrast image ( d ), Ki67 immunofluorescence ( e ), and a merged image with DAPI nuclear counterstain ( f ). ( g , h ) Representative metaphase spreads showing a normal diploid karyotype (40 chromosomes) ( g ) and a near-tetraploid karyotype (78 chromosomes) from the IMG-A1 line ( h ). ( i – k ) Ploidy analysis by flow cytometry. Control blood cells ( i ) and primary granulosa cells ( j ) show characteristic diploid (2n) and tetraploid (4n, G2/M phase) peaks. The IMG-A1 culture ( k ) displays a predominantly near-tetraploid and near-octaploid cell population. ( l ) Flow cytometry analysis confirming the homogeneity of the IMG-A1 culture (passages 10 and 40) based on staining for the stromal marker CD29 and the absence of hematopoietic markers CD45, CD34, and the fibroblast marker CD90. Quadrant gates were set based on unstained controls. Scale bars = 100 µm.

Article Snippet: PE CD34 Rat mAb [RAM34] , Elabscience Biotechnology Co., Ltd., Wuhan, China , E-AB-F1284D.

Techniques: Cell Culture, Staining, Activity Assay, Marker, Immunofluorescence, Flow Cytometry, Control

Journal: Breast cancer research : BCR

Article Title: Dissecting the tumor microenvironment in primary breast angiosarcoma: insights from single-cell RNA sequencing.

doi: 10.1186/s13058-025-02022-9

Figure Lengend Snippet: Fig. 1 Imaging and pathological analysis of a breast angiosarcoma patient. MRI reveals two masses in the central and lower outer quadrants of the right breast (A). CT scan shows a large mass in the left breast and multiple masses in the right breast (B). H&E staining at 40× magnification shows primary angiosarcoma of the breast, with tumor tissue exhibiting diverse morphologies. Interconnecting slit-like structures are visible, with well-formed anastomosing blood vessels at the periphery. The central region displays a higher tumor cell density and dilated lumina (C). H&E staining at 200× magnification reveals dense areas where tumor cells grow in sheets. The cells exhibit spindle-shaped and epithelioid morphologies, with visible mitotic figures. Blood lakes are present within variably shaped and dilated blood vessels (D). Immunohistochemical staining for CD31, CD34, Factor VIII, and FLI-1 shows variable expression across these markers (E-H)

Article Snippet: Immunostaining was conducted using a Dako Autostainer (Dako Corporation, Carpinteria, CA), and the following antibodies were employed: anti-CD31 rabbit polyclonal antibody (ZA-0658, Clone EP78, Beijing Golden Bridge Biotechnology), anti-CD34 monoclonal antibody (Kit-0004, Clone QBEnd/10, Fuzhou Maixin Biotechnology), anti-Factor VIII monoclonal antibody (ZM-0022, Clone OTI9 F3, Beijing Golden Bridge Biotechnology), and anti-FLI-1 monoclonal antibody (ZM-0108, Clone G146-22, Beijing Golden Bridge Biotechnology).

Techniques: Imaging, Computed Tomography, Staining, Immunohistochemical staining, Expressing