Journal: PLoS ONE

Article Title: Serglycin in Quiescent and Proliferating Primary Endothelial Cells

doi: 10.1371/journal.pone.0145584

Figure Lengend Snippet: Antibodies.

Article Snippet: Conditioned medium of equal protein concentration (related to their corresponding cell lysates) and containing approximately 40,000 cpm were incubated over night with agitation at 4°C with monoclonal mouse anti human serglycin (H00005552-H03, Abnova, 1:500) or polyclonal rabbit anti-human serglycin (HPA000759, Atlas Antibodies, 1:100) or polyclonal rabbit anti-versican (a kind gift from Dieter Zimmermann, University Hospital Zurich).

Techniques: Western Blot, Plasmid Preparation

Journal: Cancer Management and Research

Article Title: By Increasing the Expression and Activation of STAT3, Sustained C5a Stimulation Increases the Proliferation, Migration, and Invasion of RCC Cells and Promotes the Growth of Transgrafted Tumors

doi: 10.2147/CMAR.S326352

Figure Lengend Snippet: Secretory over-expression of C5a significantly increased the expression and activation of JNK/STAT3 pathway. The level of AKT ( A ), phosphorylated AKT (p-AKT, ( B ), ERK1/2 ( C ), phosphorylated ERK1/2 (p-ERK1/2, ( D ), p38 ( E ), phosphorylated p38 (p-p38, ( F ), STAT3 ( G ), phosphorylated STAT3 (p-STAT3, ( H ) and phosphorylated JNK (p-SAPK/JNK, ( I ) were measured by Western blot method. All the experiments were repeated three times. **P<0.01 vs 786-O group in ( G, H and I ).

Article Snippet: These antibodies include rabbit anti-human C5aR1 (Ls-B5559, dilution of 1:1000; Lifespan, Seattle, WA, USA), rabbit anti-human AKT (4691S, dilution of 1:1000; Cell Signaling Technology, MA, USA), rabbit anti-human p-AKT (4060S, dilution of 1:1000; Cell Signaling Technology, MA, USA), rabbit anti-human β-actin (YT0099, dilution of 1:5000; Immunoway, TX, USA), rabbit anti-human ERK1/2 (4695S, dilution of 1:1000; Cell Signaling Technology, MA, USA), rabbit anti-human p-ERK1/2 (4370S, dilution of 1:1000; Cell Signaling Technology, MA, USA), rabbit anti-human p38 (8690S, dilution of 1:1000; Cell Signaling Technology, MA, USA), rabbit anti-human p-p38 (4511S, dilution of 1:1000; Cell Signaling Technology, MA, USA), mouse anti-human STAT3 (9139T, dilution of 1:1000; Cell Signaling Technology, MA, USA), rabbit anti-human p-STAT3 (9145T, dilution of 1:1000; Cell Signaling Technology, MA, USA), rabbit anti-human p-SAPK/JNK (4668T, dilution of 1:1000; Cell Signaling Technology, MA, USA), mouse anti-human GAPDH (YM3029, dilution of 1:10000; Immunoway, TX, USA), rabbit anti-human CPD (NBP1-91447, dilution of 1:1000; Novus Biologicals, Centennial, CO, USA), rabbit anti-human CPM (NBP1-87403, dilution of 1:1000; Novus Biologicals, Centennial, CO, USA), rabbit anti-human CPN (Ls-c166993, dilution of 1:1000; Lifespan, Seattle, WA, USA) and rabbit anti-human CPZ (Ls-199274, dilution of 1:1000; Lifespan, Seattle, WA, USA).

Techniques: Over Expression, Expressing, Activation Assay, Western Blot

Journal: Cancer Management and Research

Article Title: By Increasing the Expression and Activation of STAT3, Sustained C5a Stimulation Increases the Proliferation, Migration, and Invasion of RCC Cells and Promotes the Growth of Transgrafted Tumors

doi: 10.2147/CMAR.S326352

Figure Lengend Snippet: Inhibition of STAT3 blocked the effect of secretory C5a over-expression on the proliferation, migration, and invasion of RCC cells. The STAT3 specific inhibitor Stattic (20 μM) was used in the assay. The influence of Stattic on cellular proliferation ( A ), migration ( B and C ), and invasion ( D and E ) of untransfected 786-O and secretory C5a over-expression 786-O cells were analyzed by using CCK8, scratch healing and Matrigel invasion chamber methods. All the experiments were repeated at least three times. **P<0.01 vs O group. ## P<0.01 vs O-C5a group. Scale bar: 100 µm.

Article Snippet: These antibodies include rabbit anti-human C5aR1 (Ls-B5559, dilution of 1:1000; Lifespan, Seattle, WA, USA), rabbit anti-human AKT (4691S, dilution of 1:1000; Cell Signaling Technology, MA, USA), rabbit anti-human p-AKT (4060S, dilution of 1:1000; Cell Signaling Technology, MA, USA), rabbit anti-human β-actin (YT0099, dilution of 1:5000; Immunoway, TX, USA), rabbit anti-human ERK1/2 (4695S, dilution of 1:1000; Cell Signaling Technology, MA, USA), rabbit anti-human p-ERK1/2 (4370S, dilution of 1:1000; Cell Signaling Technology, MA, USA), rabbit anti-human p38 (8690S, dilution of 1:1000; Cell Signaling Technology, MA, USA), rabbit anti-human p-p38 (4511S, dilution of 1:1000; Cell Signaling Technology, MA, USA), mouse anti-human STAT3 (9139T, dilution of 1:1000; Cell Signaling Technology, MA, USA), rabbit anti-human p-STAT3 (9145T, dilution of 1:1000; Cell Signaling Technology, MA, USA), rabbit anti-human p-SAPK/JNK (4668T, dilution of 1:1000; Cell Signaling Technology, MA, USA), mouse anti-human GAPDH (YM3029, dilution of 1:10000; Immunoway, TX, USA), rabbit anti-human CPD (NBP1-91447, dilution of 1:1000; Novus Biologicals, Centennial, CO, USA), rabbit anti-human CPM (NBP1-87403, dilution of 1:1000; Novus Biologicals, Centennial, CO, USA), rabbit anti-human CPN (Ls-c166993, dilution of 1:1000; Lifespan, Seattle, WA, USA) and rabbit anti-human CPZ (Ls-199274, dilution of 1:1000; Lifespan, Seattle, WA, USA).

Techniques: Inhibition, Over Expression, Migration