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Journal: Scientific Reports
Article Title: Furin inhibitor protects against neuronal cell death induced by activated NMDA receptors
doi: 10.1038/s41598-018-23567-0
Figure Lengend Snippet: ( a ) Cortical cells were fixed at 10 DIV and stained for NeuN (green) and GFAP (red) and with Hoechst (blue). The scale bar represents 100 µm. ( b ) The number of NeuN- and GFAP-positive cells was counted. Results were expressed as the percentage of these cells among the total number of Hoechst-positive cells and as the means ± SE in 4 independent experiments. ( c ) Proteins from cultured cortical neurons at 2, 4, 6, 8, 10 and 12 DIV were analyzed by immunoblotting with anti-GluN1, anti-GluN2A, anti-GluN2B, and anti-β-actin antibodies. Bands corresponding to GluN1, GluN2A, and GluN2B were scanned, the scanned bands were normalized by each protein at 2 DIV on the same blot. β-actin was used as a loading control. Results are the means ± SE (n = 4 independent experiments).
Article Snippet: The following primary antibodies were used: mouse anti-β-actin (a5441, Sigma),
Techniques: Staining, Cell Culture, Western Blot