Journal: Experimental and Therapeutic Medicine

Article Title: Negative pressure wound therapy enhances bone regeneration compared with conventional therapy in a rabbit radius gap-healing model

doi: 10.3892/etm.2021.9905

Figure Lengend Snippet: VEGF, BMP-2 and OPN protein expression. (A) Representative western blot image and quantification of (B) VEGF, (C) BMP-2 and (D) OPN protein expression at each time point examined in the NPWT and control groups. * P<0.05 and ** P<0.01. VEGF, vascular endothelial growth factor; BMP-2, bone morphogenetic protein 2; OPN, osteopontin; NPWT, negative pressure wound therapy; 2W, week 2; 4W, week 4.

Article Snippet: The membranes were then incubated overnight at 4˚C with TBS-Tween-20 supplemented with one of the following primary antibodies: Mouse anti-β-actin (1:1,000; Boster Biological Technology; cat. no. P60709), mouse anti-VEGF (1:200; Santa Cruz Biotechnology, Inc. cat. no. sc-7269), mouse anti-BMP-2 (1:400; Santa Cruz Biotechnology, Inc. cat. no. sc-137087) or mouse anti-OPN (1:400; Abcam; cat. no. ab228748).

Techniques: Expressing, Western Blot

Journal: BMC Musculoskeletal Disorders

Article Title: Increased synovial immunohistochemistry reactivity of TGF-β1 in erosive peripheral psoriatic arthritis

doi: 10.1186/s12891-023-06339-4

Figure Lengend Snippet: Expression ratios of mRNA in the synovial membrane

Article Snippet: Indirect immunohistochemical techniques (with paraffin peroxidase) were used in all the biopsies, in which the mouse monoclonal anti-BMP2 ab6285 antibody (clone 65529.111) of Abcam® and rabbit monoclonal anti-Dkk1 antibody were used as the primary antibodies. ab109416 (clone EPR4759) from Abcam®, rabbit anti-BMP2 monoclonal antibody ab 124,715 (clone EPR6211) from Abcam®, mouse monoclonal anti-Wnt5a ab86720 (clone 3D10) from Abcam®, mouse monoclonal antibody anti-TGF-β1 ab64715 (clone 2Ar2) from Abcam®, Abcam® ab79056 rabbit anti-IL-17 A polyclonal antibody, and Dako® K-5007 antibody (EnVision TM Detection Systems Peroxidase / DAB), were used as the secondary antibodies.

Techniques: Expressing

Journal: BMC Musculoskeletal Disorders

Article Title: Increased synovial immunohistochemistry reactivity of TGF-β1 in erosive peripheral psoriatic arthritis

doi: 10.1186/s12891-023-06339-4

Figure Lengend Snippet: Correlation between gene expression of IL-17 A with IL-23, Dkk1, BMP2, and BMP4

Article Snippet: Indirect immunohistochemical techniques (with paraffin peroxidase) were used in all the biopsies, in which the mouse monoclonal anti-BMP2 ab6285 antibody (clone 65529.111) of Abcam® and rabbit monoclonal anti-Dkk1 antibody were used as the primary antibodies. ab109416 (clone EPR4759) from Abcam®, rabbit anti-BMP2 monoclonal antibody ab 124,715 (clone EPR6211) from Abcam®, mouse monoclonal anti-Wnt5a ab86720 (clone 3D10) from Abcam®, mouse monoclonal antibody anti-TGF-β1 ab64715 (clone 2Ar2) from Abcam®, Abcam® ab79056 rabbit anti-IL-17 A polyclonal antibody, and Dako® K-5007 antibody (EnVision TM Detection Systems Peroxidase / DAB), were used as the secondary antibodies.

Techniques: Gene Expression

Journal: BMC Musculoskeletal Disorders

Article Title: Increased synovial immunohistochemistry reactivity of TGF-β1 in erosive peripheral psoriatic arthritis

doi: 10.1186/s12891-023-06339-4

Figure Lengend Snippet: Differences between gene expression of IL-17 A, IL-23, Dkk1, TGF-β1, BMP2, and BMP4 SpA: spondyloarthritis (n= 18): RA: rheumatoid arthritis (n=8); OA: osteoarthritis (n=16) (*) p= 0.001

Article Snippet: Indirect immunohistochemical techniques (with paraffin peroxidase) were used in all the biopsies, in which the mouse monoclonal anti-BMP2 ab6285 antibody (clone 65529.111) of Abcam® and rabbit monoclonal anti-Dkk1 antibody were used as the primary antibodies. ab109416 (clone EPR4759) from Abcam®, rabbit anti-BMP2 monoclonal antibody ab 124,715 (clone EPR6211) from Abcam®, mouse monoclonal anti-Wnt5a ab86720 (clone 3D10) from Abcam®, mouse monoclonal antibody anti-TGF-β1 ab64715 (clone 2Ar2) from Abcam®, Abcam® ab79056 rabbit anti-IL-17 A polyclonal antibody, and Dako® K-5007 antibody (EnVision TM Detection Systems Peroxidase / DAB), were used as the secondary antibodies.

Techniques: Gene Expression

Journal: BMC Musculoskeletal Disorders

Article Title: Increased synovial immunohistochemistry reactivity of TGF-β1 in erosive peripheral psoriatic arthritis

doi: 10.1186/s12891-023-06339-4

Figure Lengend Snippet: Differences between gene expression of IL-17 A, IL-23, Dkk1, TGF-β1, BMP2, and BMP4 (excluding ankylosing spondylitis patients) PsA: psoriatic arthritis (n= 14); RA: rheumatoid arthritis (n=8); OA: osteoarthritis (n=16) (*) p= 0.018

Article Snippet: Indirect immunohistochemical techniques (with paraffin peroxidase) were used in all the biopsies, in which the mouse monoclonal anti-BMP2 ab6285 antibody (clone 65529.111) of Abcam® and rabbit monoclonal anti-Dkk1 antibody were used as the primary antibodies. ab109416 (clone EPR4759) from Abcam®, rabbit anti-BMP2 monoclonal antibody ab 124,715 (clone EPR6211) from Abcam®, mouse monoclonal anti-Wnt5a ab86720 (clone 3D10) from Abcam®, mouse monoclonal antibody anti-TGF-β1 ab64715 (clone 2Ar2) from Abcam®, Abcam® ab79056 rabbit anti-IL-17 A polyclonal antibody, and Dako® K-5007 antibody (EnVision TM Detection Systems Peroxidase / DAB), were used as the secondary antibodies.

Techniques: Gene Expression

Journal: BMC Musculoskeletal Disorders

Article Title: Increased synovial immunohistochemistry reactivity of TGF-β1 in erosive peripheral psoriatic arthritis

doi: 10.1186/s12891-023-06339-4

Figure Lengend Snippet: IHC reactivity differences according to the diseases

Article Snippet: Indirect immunohistochemical techniques (with paraffin peroxidase) were used in all the biopsies, in which the mouse monoclonal anti-BMP2 ab6285 antibody (clone 65529.111) of Abcam® and rabbit monoclonal anti-Dkk1 antibody were used as the primary antibodies. ab109416 (clone EPR4759) from Abcam®, rabbit anti-BMP2 monoclonal antibody ab 124,715 (clone EPR6211) from Abcam®, mouse monoclonal anti-Wnt5a ab86720 (clone 3D10) from Abcam®, mouse monoclonal antibody anti-TGF-β1 ab64715 (clone 2Ar2) from Abcam®, Abcam® ab79056 rabbit anti-IL-17 A polyclonal antibody, and Dako® K-5007 antibody (EnVision TM Detection Systems Peroxidase / DAB), were used as the secondary antibodies.

Techniques:

Journal: BMC Musculoskeletal Disorders

Article Title: Increased synovial immunohistochemistry reactivity of TGF-β1 in erosive peripheral psoriatic arthritis

doi: 10.1186/s12891-023-06339-4

Figure Lengend Snippet: Serum levels of proteins according to the diseases

Article Snippet: Indirect immunohistochemical techniques (with paraffin peroxidase) were used in all the biopsies, in which the mouse monoclonal anti-BMP2 ab6285 antibody (clone 65529.111) of Abcam® and rabbit monoclonal anti-Dkk1 antibody were used as the primary antibodies. ab109416 (clone EPR4759) from Abcam®, rabbit anti-BMP2 monoclonal antibody ab 124,715 (clone EPR6211) from Abcam®, mouse monoclonal anti-Wnt5a ab86720 (clone 3D10) from Abcam®, mouse monoclonal antibody anti-TGF-β1 ab64715 (clone 2Ar2) from Abcam®, Abcam® ab79056 rabbit anti-IL-17 A polyclonal antibody, and Dako® K-5007 antibody (EnVision TM Detection Systems Peroxidase / DAB), were used as the secondary antibodies.

Techniques:

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: FBS-Derived Exosomes as a Natural Nano-Scale Carrier for Icariin Promote Osteoblast Proliferation

doi: 10.3389/fbioe.2021.615920

Figure Lengend Snippet: Effects of ICA on osteoblast proliferation. (A) ICA (0.1 μg/mL) promoted osteoblast proliferation; (B) Time dependence of the effect of ICA on osteoblast proliferation activity; and (C) Increased protein expression of BMP-2, RUNX2, and OPN after ICA was applied to MC3T3-E1 cells. * P -value < 0.05 ( n = 4).

Article Snippet: The membranes were incubated overnight at 4°C with specific anti-CD63 (diluted 1: 200; Abcam, United States), anti-CD81 (diluted 1: 500; Abcam, United States), anti-CD40 (diluted 1: 1000; Bioss, China), anti-ALIX (diluted 1: 1000; Abbexa, United Kingdom), anti-RUNX2 (diluted 1: 500, SAB, United States), anti-BMP-2 (diluted 1: 500, Bioworld, United States), anti-OPN (diluted 1: 1000, Proteintech, United States), and anti-GAPDH (diluted 1: 5000, Bioworld, United States).

Techniques: Activity Assay, Expressing

Journal: International Journal of Molecular Sciences

Article Title: The Hematopoietic TALE-Code Shows Normal Activity of IRX1 in Myeloid Progenitors and Reveals Ectopic Expression of IRX3 and IRX5 in Acute Myeloid Leukemia

doi: 10.3390/ijms23063192

Figure Lengend Snippet: Transcriptional regulation of IRX3 and IRX5. ( A ) RQ-PCR analysis of HOXA10 in AML cell lines ( left ). Transcript levels are indicated in relation to OCI-AML3, and IRX3/IRX5-positive cell lines are indicated in red. RQ-PCR analysis of OCI-AML3 ( above ) and MEGAL ( below ) showed downregulation of IRX3 and IRX5 after siRNA-mediated knockdown of HOXA10 ( right ). ( B ) RQ-PCR analysis of BMP2 in AML cell lines ( left ). Transcript levels are indicated in relation to OCI-AML3. ELISA results for BMP2 protein levels in AML cell line supernatants are inserted. RQ-PCR analysis of OCI-AML3 treated with BMP2 resulted in the upregulation of IRX3 and IRX5 ( right ). ( C ) RQ-PCR analysis of OCI-AML3 after siRNA-mediated knockdown of JUNB ( left ) and SMAD4 ( right ) showed downregulation of IRX3 and IRX5. ( D ) RQ-PCR analysis of JUNB in AML cell lines ( left ). Transcript levels are indicated in relation to OCI-AML3. RQ-PCR analysis of OCI-AML3 treated with inhibitory BMP2-antibody resulted in downregulation of JUNB, IRX3, and IRX5, while HOXA10 was not significantly affected ( right ). p -values are indicated by asterisks (* p < 0.05, ** p < 0.01, *** p < 0.001, n.s. not significant).

Article Snippet: Cell treatments were performed for 20 h using 20 ng/mL of recombinant BMP2 (R & D Systems, Wiesbaden, Germany, #355-BM-010/CF) or 20 μg/mL of inhibitory monoclonal anti-BMP2 antibody (R & D Systems, #MAB3552).

Techniques: Knockdown, Enzyme-linked Immunosorbent Assay

Journal: International Journal of Molecular Sciences

Article Title: The Hematopoietic TALE-Code Shows Normal Activity of IRX1 in Myeloid Progenitors and Reveals Ectopic Expression of IRX3 and IRX5 in Acute Myeloid Leukemia

doi: 10.3390/ijms23063192

Figure Lengend Snippet: Gene regulatory network of IRX1 and IRX3/IRX5 in AML. The figure summarizes the results of this study. TALE homeobox genes IRX1, IRX3, and IRX5 are located centrally, chromosomal aberrations lie upstream, and developmental TFs and BMP2-signaling components both upstream and downstream. Thus, these IRX genes are part of a regulatory gene network, controlling myeloid differentiation.

Article Snippet: Cell treatments were performed for 20 h using 20 ng/mL of recombinant BMP2 (R & D Systems, Wiesbaden, Germany, #355-BM-010/CF) or 20 μg/mL of inhibitory monoclonal anti-BMP2 antibody (R & D Systems, #MAB3552).

Techniques:

Journal: Scientific Reports

Article Title: Increased EZH2 and decreased osteoblastogenesis during local irradiation-induced bone loss in rats

doi: 10.1038/srep31318

Figure Lengend Snippet: ( a , b ) Immunohistochemistry analysis (integrated optical density, IOD) of EZH2 expression in bone marrow at 2 weeks ( a ) and 12 weeks ( b ) after irradiation ( n = 6/group). Original magnification, ×200. ( c , d ) mRNA expression of EZH2, BMP2 and Runx2 at 2 weeks ( c ) and 12 weeks ( d ) after irradiation ( n = 6/group). ( e , f ) Protein expression of EZH2, Runx2 and BMP2 at 2 weeks ( e ) and 12 weeks ( f ) after irradiation ( n = 4/group). The data are presented as the mean ± standard deviation. * P < 0.05, ** P < 0.01 and *** P < 0.001.

Article Snippet: The membranes were blocked with 5% fat-free dry milk in TBST (0.1% Tween-20 and 0.1 M NaCl in 0.1 M Tris–HCl, pH 7.5) for 2 h at room temperature and with rabbit anti-EZH2 antibody (5246s, Cell Signaling Technology, USA, 1:1,000), rabbit anti-BMP2 antibody (13105s, Cell Signaling Technology, USA, 1:1,000), goat anti-Runx2 antibody (ab56326, Abcam, Cambridge, MA, 1:1,000) and mouse anti-GAPDH antibody (Kangcheng, Shanghai, China, 1:5,000) at 4 °C overnight.

Techniques: Immunohistochemistry, Expressing, Irradiation, Standard Deviation