Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Maternal and Foetal Cellular Immune Responses in Dams Infected With High- and Low- Virulence Isolates of Neospora caninum at Mid-Gestation

doi: 10.3389/fcimb.2021.684670

Figure Lengend Snippet: List of antibodies used for analysis of cell subsets in PBMC, ileofemoral lymph node, fetal thymus and spleen.

Article Snippet: CD14 , FITC , Bio-Rad Laboratories (Pleasanton, CA, USA) , Mouse anti-bovine , Monoclonal , CC-G33 , MCA2678F.

Techniques:

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Maternal and Foetal Cellular Immune Responses in Dams Infected With High- and Low- Virulence Isolates of Neospora caninum at Mid-Gestation

doi: 10.3389/fcimb.2021.684670

Figure Lengend Snippet: Relative percentage of immune cells in a PBMC population from Nc-Spain7- and Nc-Spain1H-infected heifers. Graphs indicate the relative percentage of cells positive for CD21, WC1, CD4, CD8, CD14 and CD335 surface markers, and CD4+/CD8+ ratio in PBMC obtained from uninfected heifers (G-Control) and heifers challenged with Nc-Spain7 (G-NcSpain7) and Nc-Spain1H (G-NcSpain1H) tachyzoites. The results are represented as the mean + SD. Asterisks indicate significant differences in G-NcSpain1H versus G-Control comparison (blue), G-NcSpain7 versus G-Control (red) and G-NcSpain1H versus G-NcSpain7 (black). **** P < 0.0001, *** P < 0.001, ** P < 0.01, * P < 0.05.

Article Snippet: CD14 , FITC , Bio-Rad Laboratories (Pleasanton, CA, USA) , Mouse anti-bovine , Monoclonal , CC-G33 , MCA2678F.

Techniques: Infection, Control, Comparison

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Maternal and Foetal Cellular Immune Responses in Dams Infected With High- and Low- Virulence Isolates of Neospora caninum at Mid-Gestation

doi: 10.3389/fcimb.2021.684670

Figure Lengend Snippet: Relative percentage of immune cells in iliofemoral lymph nodes from Nc-Spain7- and Nc-Spain1H-infected heifers. Graphs indicate the relative percentage of cells positive for CD21, WC1, CD4, CD8, CD14 and CD335 surface markers, and CD4+/CD8+ ratio surface markers in the population of immune cells obtained from the iliofemoral lymph node of uninfected heifers (G-Control) and heifers challenged with Nc-Spain7 (G-NcSpain7) and Nc-Spain1H (G-NcSpain1H) tachyzoites culled at 10 or 20 dpi. The results are represented as the mean + SD. Asterisks indicate significant differences. ** P < 0.01, * P < 0.05.

Article Snippet: CD14 , FITC , Bio-Rad Laboratories (Pleasanton, CA, USA) , Mouse anti-bovine , Monoclonal , CC-G33 , MCA2678F.

Techniques: Infection, Control

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Maternal and Foetal Cellular Immune Responses in Dams Infected With High- and Low- Virulence Isolates of Neospora caninum at Mid-Gestation

doi: 10.3389/fcimb.2021.684670

Figure Lengend Snippet: Immune cell populations in the spleen of foetuses from Nc-Spain7 and Nc-Spain1H infected heifers at 10 and 20 dpi. Graphs indicate the relative percentage of cells positive for CD21, WC1, CD4, CD8, CD14 and CD335 surface markers, double positive (DP) CD4+CD8+ cells and CD4+/CD8+ ratio, in the population of immune cells obtained from the spleen of foetuses from uninfected heifers (G-Control) and heifers challenged with Nc-Spain7 (G-NcSpain7) and Nc-Spain1H (G-NcSpain1H) tachyzoites culled at 10 or 20 dpi. The results are represented as the mean + SD. Asterisks indicate significant differences. ** P < 0.01, * P < 0.05.

Article Snippet: CD14 , FITC , Bio-Rad Laboratories (Pleasanton, CA, USA) , Mouse anti-bovine , Monoclonal , CC-G33 , MCA2678F.

Techniques: Infection, Control

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Maternal and Foetal Cellular Immune Responses in Dams Infected With High- and Low- Virulence Isolates of Neospora caninum at Mid-Gestation

doi: 10.3389/fcimb.2021.684670

Figure Lengend Snippet: Immune cell populations in the thymus of foetuses from Nc-Spain7 and Nc-Spain1H infected heifers at 10 and 20 dpi. Graphs indicate the relative percentage of cells positive for CD21, WC1, CD4, CD8, CD14 and CD335 surface markers, double positive (DP) CD4+CD8+ cells and CD4+/CD8+ ratio, in the population of immune cells obtained from the thymus of foetuses from uninfected heifers (G-Control) and heifers challenged with Nc-Spain7 (G-NcSpain7) and Nc-Spain1H (G-NcSpain1H) tachyzoites culled at 10 or 20 dpi. The results are represented as the mean + SD. Asterisks indicate significant differences. * P < 0.05.

Article Snippet: CD14 , FITC , Bio-Rad Laboratories (Pleasanton, CA, USA) , Mouse anti-bovine , Monoclonal , CC-G33 , MCA2678F.

Techniques: Infection, Control

Journal: STAR Protocols

Article Title: Murine brain tumor microenvironment immunophenotyping using mass cytometry

doi: 10.1016/j.xpro.2022.101357

Figure Lengend Snippet: Antibody master mix

Article Snippet: Anti-Mouse CD14 (Sa14-2)-156Gd—100 Tests , Fluidigm , SKU# 3156009B.

Techniques:

Journal: STAR Protocols

Article Title: Murine brain tumor microenvironment immunophenotyping using mass cytometry

doi: 10.1016/j.xpro.2022.101357

Figure Lengend Snippet:

Article Snippet: Anti-Mouse CD14 (Sa14-2)-156Gd—100 Tests , Fluidigm , SKU# 3156009B.

Techniques: Purification, Recombinant, Red Blood Cell Lysis, Centrifugation, Staining, Electron Microscopy, Antibody Labeling, Software, Sterility, Spectrophotometry

Journal: PeerJ

Article Title: Main histological parameters to be evaluated in an experimental model of myocardial infarct treated by stem cells on pigs

doi: 10.7717/peerj.7160

Figure Lengend Snippet: The number of macrophages (arrow) in the peri-cicatricial area of MI+ASC heart (A) is similar to MIC group (B). Immunohistochemistry anti-CD14, PAP. 400 ×. M2 macrophages (arrows) are in greater proportion in MI+ASC group (C) than in MIC (D). Immunohistochemistry anti-CD163 PAP. 400 ×.

Article Snippet: The primary antibodies used were anti-CD14 antibody (MyBioSource, MBS-2027456, 1/500), anti-CD163 antibody (Serotec, MCA242GA, 1/200), muscle specific actin monoclonal antibody (Novocastra, A7811, 1/100), Desmin Monoclonal Antibody (Novocastra, DE-R11, 1/50), Connexin 43 mouse monoclonal antibody (Cell Signaling Technology, cst-3512, 1/50), and HIF1-α antibody (Gene Tex, GTX 30105, 1/1000).

Techniques: Immunohistochemistry