Journal: iScience

Article Title: Crystallization as a selection force at the polymerization of nucleotides in a prebiotic context.

doi: 10.1016/j.isci.2023.107600

Figure Lengend Snippet: Figure 3. Evaporative crystallization from a 1:1 mixture of 30,50cGMP-H with 30,50cAMP-H or 50GMP-H (A) Crystallization of 30,50cGMP-H$5H2O from a 1:1 mixture of 30,50cGMP-H and 30,50cAMP-H. (B) Electrophoretic image of the polymerization products formed upon incubation (80C, 5 h) of a dry material containing 30,50cGMP-H (lane 1), 30,50cAMP-H (lane 2) and a 1:1 mixture of 30,50cGMP-H and 30,50cAMP-H (lane 3; fast migrating low-molecular weight [<3nt] fragments likely correspond to depurinated oligomers). (C) Crystallization of 30,50cGMP-H$5H2O from a 1:1 mixture of 30,50cGMP-H and 50GMP-H. (D) Crystallization of 30,50cGMP-H$5H2O from a 1:1:2 mixture of 30,50cGMP-H, 50GMP (disodium salt form) and HCl.

Article Snippet: Chemicals, peptides, and recombinant proteins Free acid form 30,50cGMP Biolog LSI, Bremen custom made Free acid form 30,50cAMP Biolog LSI, Bremen custom made Free acid form 30,50cCMP Biolog LSI, Bremen custom made 30,50cGMP, sodium salt Sigma-Aldrich Cat. # G6129 Free acid form 50AMP Merck Cat.# A-2252 Free acid form 50UMP Merck Cat.# U-1752 Free acid form 50GMP Santa Cruz Biotech Cat.# SC-295032A 50GMP, disodium salt Merck Cat.# G-8377 Glycine Merck Cat. # 50046 Hydrochloric acid, conc.

Techniques: Crystallization Assay, Incubation, Molecular Weight