Journal: Nature Communications

Article Title: Endothelial C-type natriuretic peptide/guanylyl cyclase-B signaling prevents pulmonary arterial hypertension

doi: 10.1038/s41467-026-70139-2

Figure Lengend Snippet: RT-qPCR analyses of the whole lung expression of Nppc ( a , b ), Npr2 ( c , d ), Npr1 ( e , f ), Edn1 ( g , h ), Il6 ( i , j ), Ccl2 ( k , l ), and Tgfb1 ( m , n ) in Tie2-Cre, SM22α-Cre, CNP ecKO, GC-B ecKO and GC-B smcKO mice in the MCTp-induced model (10-week-old, a , c , e , g , i , k , m ) and Hx-induced model (12-week-old, b , d , f , h , j , l , n ) compared with untreated controls (10-week-old of MCTp (–) and 12-week-old normoxia; n = 8 per group). GAPDH was used as the internal control. Data are presented as the mean ± SEM. P values were calculated using one-way ANOVA with Tukey’s multiple comparisons test.

Article Snippet: For the monocrotaline pyrrole (MCTp)-induced PAH model, 5-mg aliquots of MCTp (Lot J0917, I2818, SC-214861A; Santa Cruz Biotechnology, Dallas, TX, USA) were dissolved in N,N -dimethylformamide (DMF) and stored at –80 °C until use , .

Techniques: Quantitative RT-PCR, Expressing, Control

Journal: Nature Communications

Article Title: Endothelial C-type natriuretic peptide/guanylyl cyclase-B signaling prevents pulmonary arterial hypertension

doi: 10.1038/s41467-026-70139-2

Figure Lengend Snippet: a PASMC proliferation assessed using CCK-8 assays under hypoxia. CM from GC-B-deficient HPAECs (EC+siGC-B) significantly increased PASMC proliferation compared with CM from control HPAECs (EC control). Proliferation was quantified in PASMCs ( n = 12 biological replicates, independent culture wells , per group). b Schematic representation of the Boyden chamber co-culture assay. PASMCs (blue) were seeded into the upper insert, while HPAECs (red) were cultured in the lower chamber to evaluate endothelial-smooth muscle paracrine interactions. c PASMC migration measured by Boyden chamber assays under hypoxia. Migrated cells were quantified based on fluorescence intensity. Migration was significantly increased in response to CM from EC+siGC-B compared with control CM ( n = 6 biological replicates, independent culture wells , per group). Representative Ki67 staining ( d ) and quantification ( e ) of PASMC proliferation in small pulmonary arteries from CNP ecKO mice in the MCTp-induced PAH model, treated with vehicle (Control), MCTp (PAH model) or MCTp + CNP (PAH model + CNP). Arrowheads indicate Ki67-positive nuclei ( n = 10 per group). Representative TUNEL staining ( f ) and quantification ( g ) of PASMC apoptosis in the same groups. Arrowheads indicate TUNEL-positive nuclei ( n = 10 per group). Data are presented as the mean ± SEM. P values were calculated using one-way ANOVA with Tukey’s multiple comparisons test.

Article Snippet: For the monocrotaline pyrrole (MCTp)-induced PAH model, 5-mg aliquots of MCTp (Lot J0917, I2818, SC-214861A; Santa Cruz Biotechnology, Dallas, TX, USA) were dissolved in N,N -dimethylformamide (DMF) and stored at –80 °C until use , .

Techniques: CCK-8 Assay, Control, Co-culture Assay, Cell Culture, Migration, Fluorescence, Staining, TUNEL Assay

Journal: Nature Communications

Article Title: Endothelial C-type natriuretic peptide/guanylyl cyclase-B signaling prevents pulmonary arterial hypertension

doi: 10.1038/s41467-026-70139-2

Figure Lengend Snippet: Effect of continuous subcutaneous infusion of CNP-53 (0.2 mg/kg/day) on systemic BP in Tie2-Cre control, GC-B ecKO and GC-B smcKO mice in the MCTp-induced model ( a ; 10-week-old) and Hx-induced model ( b ; 12-week-old) ( n = 3 per group). Effects of CNP-53 infusion on RVSP ( c , e ) and RV/(LV + IVS) ratios ( d , f ) in Tie2-Cre mice under MCTp ( c , d ; 10-week-old) and Hx ( e , f ; 12-week-old) conditions (MCTp model: n = 5 for untreated [MCTp (–)/CNP (–)], n = 5 for MCTp (+)/CNP (–), n = 3 for MCTp (+)/CNP (+); Hx model: n = 5 for normoxia/CNP (–), n = 5 for Hx/CNP (–), n = 3 for Hx/CNP (+)). Effects of CNP-53 infusion on RVSP ( g , i ) and RV/(LV + IVS) ratios ( h , j ) in GC-B ecKO and GC-B smcKO mice under MCTp ( g , h ; 10-week-old) and Hx ( i , j ; 12-week-old) conditions (MCTp model: n = 5 for MCTp (–)/CNP (–), n = 5 for MCTp (+)/CNP (–), n = 3 for MCTp (+)/CNP (+); Hx model: n = 5 for normoxia/CNP (–), n = 5 for Hx/CNP (–), n = 3 for Hx/CNP (+)). Data are presented as the mean ± SEM. P values were calculated using one-way ANOVA with Tukey’s multiple comparisons test.

Article Snippet: For the monocrotaline pyrrole (MCTp)-induced PAH model, 5-mg aliquots of MCTp (Lot J0917, I2818, SC-214861A; Santa Cruz Biotechnology, Dallas, TX, USA) were dissolved in N,N -dimethylformamide (DMF) and stored at –80 °C until use , .

Techniques: Control

Journal: Nature Communications

Article Title: Endothelial C-type natriuretic peptide/guanylyl cyclase-B signaling prevents pulmonary arterial hypertension

doi: 10.1038/s41467-026-70139-2

Figure Lengend Snippet: a PASMC proliferation assessed using CCK-8 assays under hypoxia. CM from GC-B-deficient HPAECs (EC+siGC-B) significantly increased PASMC proliferation compared with CM from control HPAECs (EC control). Proliferation was quantified in PASMCs ( n = 12 biological replicates, independent culture wells , per group). b Schematic representation of the Boyden chamber co-culture assay. PASMCs (blue) were seeded into the upper insert, while HPAECs (red) were cultured in the lower chamber to evaluate endothelial-smooth muscle paracrine interactions. c PASMC migration measured by Boyden chamber assays under hypoxia. Migrated cells were quantified based on fluorescence intensity. Migration was significantly increased in response to CM from EC+siGC-B compared with control CM ( n = 6 biological replicates, independent culture wells , per group). Representative Ki67 staining ( d ) and quantification ( e ) of PASMC proliferation in small pulmonary arteries from CNP ecKO mice in the MCTp-induced PAH model, treated with vehicle (Control), MCTp (PAH model) or MCTp + CNP (PAH model + CNP). Arrowheads indicate Ki67-positive nuclei ( n = 10 per group). Representative TUNEL staining ( f ) and quantification ( g ) of PASMC apoptosis in the same groups. Arrowheads indicate TUNEL-positive nuclei ( n = 10 per group). Data are presented as the mean ± SEM. P values were calculated using one-way ANOVA with Tukey’s multiple comparisons test.

Article Snippet: For the monocrotaline pyrrole (MCTp)-induced PAH model, 5-mg aliquots of MCTp (Lot J0917, I2818, SC-214861A; Santa Cruz Biotechnology, Dallas, TX, USA) were dissolved in N,N -dimethylformamide (DMF) and stored at –80 °C until use , .

Techniques: CCK-8 Assay, Control, Co-culture Assay, Cell Culture, Migration, Fluorescence, Staining, TUNEL Assay