Journal: Blood and Lymphatic Cancer: Targets and Therapy

Article Title: Enzymatic Laccase Nanoreactors Induce Apoptosis in MOLT-4-ALL Cells and Activate Prodrugs in a Synergetic Effect

doi: 10.2147/BLCTT.S576292

Figure Lengend Snippet: Curve dose-response on MOLT-4 leukemia model cells. ( A ) Pro-drugs and ( B ) laccase from C. gallica .

Article Snippet: The MOLT-4 cell line, derived from human T cells originating from acute lymphoblastic leukemia, was obtained from a certified cell bank (ATCC, MOLT-4-CRL-1582).

Techniques:

Journal: Blood and Lymphatic Cancer: Targets and Therapy

Article Title: Enzymatic Laccase Nanoreactors Induce Apoptosis in MOLT-4-ALL Cells and Activate Prodrugs in a Synergetic Effect

doi: 10.2147/BLCTT.S576292

Figure Lengend Snippet: Flow cytometry analysis to determine cell viability. The green zone indicates the death cells with a stain-positive PI, and the red zone indicates the alive cell with a stain-positive FDA. Every panel corresponds to a different treatment of MOLT-4-ALL cell: ( A ) Doxorubicin, ( B ) Doxorubicin + Lac, ( C ) Doxorubicin + VLP-saLac, ( D ) Irinotecan, ( E ) Irinotecan + Lac, ( F ) Irinotecan + VLP-saLac; ( G ) Procarbazine, ( H ) Procarbazine + Lac, ( I ) Procarbazine + VLP-saLac. The percentage in every square indicates the percentage of total events.

Article Snippet: The MOLT-4 cell line, derived from human T cells originating from acute lymphoblastic leukemia, was obtained from a certified cell bank (ATCC, MOLT-4-CRL-1582).

Techniques: Flow Cytometry, Staining

Journal: International Journal of Molecular and Cellular Medicine

Article Title: Sesamin Acts as Anti-leukemic Compound Interacting with Novel Phosphoprotein Targets and Inducing Apoptosis in Leukemic Cells

doi: 10.22088/IJMCM.BUMS.11.1.1

Figure Lengend Snippet: Cytotoxic effects of sesamin on (a) MOLT-4 and (b) NB4 cell line after 24 hour and 48 hour treatments compare with PBMC cells after 48 hour treatment. The results showed the percentage of cell inhibition measured by MTT assay. IC 50 at 48 hours for MOLT-4 and NB4 were reported as 104.84 and 121.00 µg/mL, respectively. Data were expressed as the mean±SD of independently three experiments. The differences between sesamin treated and untreated were examined for the statistical significance: *, p < 0.05; **, p < 0.01; ***, p < 0.001

Article Snippet: Leukemic Cell culture Acute lymphoblastic leukemia (ALL) MOLT-4 and acute promyelocytic leukemia (AML) NB4 cells purchased from Cell Lines Service GmbH (Eppelheim, Germany) were cultured in 10% fetal bovine serum and 2% penicillin-streptomycin supplements in RPMI-1640 medium at the condition of 37 o C and 5% CO 2 .

Techniques: Inhibition, MTT Assay

Journal: International Journal of Molecular and Cellular Medicine

Article Title: Sesamin Acts as Anti-leukemic Compound Interacting with Novel Phosphoprotein Targets and Inducing Apoptosis in Leukemic Cells

doi: 10.22088/IJMCM.BUMS.11.1.1

Figure Lengend Snippet: The relative normalized expression of apoptotic genes in (a) MOLT-4 and (b) NB4 after sesamin treatment at IC 50 values of 100 and 120 µg/mL, respectively, for 48 hours. The experiment was determined by Real-time PCR. Data were expressed as the mean±SEM of three independent experiments. The differences between sesamin treated and untreated were examined for the statistical significance: *, p < 0.05; **, p < 0.01

Article Snippet: Leukemic Cell culture Acute lymphoblastic leukemia (ALL) MOLT-4 and acute promyelocytic leukemia (AML) NB4 cells purchased from Cell Lines Service GmbH (Eppelheim, Germany) were cultured in 10% fetal bovine serum and 2% penicillin-streptomycin supplements in RPMI-1640 medium at the condition of 37 o C and 5% CO 2 .

Techniques: Expressing, Real-time Polymerase Chain Reaction

Journal: International Journal of Molecular and Cellular Medicine

Article Title: Sesamin Acts as Anti-leukemic Compound Interacting with Novel Phosphoprotein Targets and Inducing Apoptosis in Leukemic Cells

doi: 10.22088/IJMCM.BUMS.11.1.1

Figure Lengend Snippet: Venn diagram shows a set of 14 phosphoproteins present only in sesamin treated MOLT-4 (blue) and a different set of 14 phosphoproteins present only in sesamin treated NB4 (yellow)

Article Snippet: Leukemic Cell culture Acute lymphoblastic leukemia (ALL) MOLT-4 and acute promyelocytic leukemia (AML) NB4 cells purchased from Cell Lines Service GmbH (Eppelheim, Germany) were cultured in 10% fetal bovine serum and 2% penicillin-streptomycin supplements in RPMI-1640 medium at the condition of 37 o C and 5% CO 2 .

Techniques:

Journal: International Journal of Molecular and Cellular Medicine

Article Title: Sesamin Acts as Anti-leukemic Compound Interacting with Novel Phosphoprotein Targets and Inducing Apoptosis in Leukemic Cells

doi: 10.22088/IJMCM.BUMS.11.1.1

Figure Lengend Snippet: Heatmap of phosphoproteins with significant difference in expression of (A) MOLT-4 and (B) NB4 treated with sesamin in various times compare with the control. The color indicates the expression level from low (green) to high (red). The blue arrow indicates the selected phosphoproteins

Article Snippet: Leukemic Cell culture Acute lymphoblastic leukemia (ALL) MOLT-4 and acute promyelocytic leukemia (AML) NB4 cells purchased from Cell Lines Service GmbH (Eppelheim, Germany) were cultured in 10% fetal bovine serum and 2% penicillin-streptomycin supplements in RPMI-1640 medium at the condition of 37 o C and 5% CO 2 .

Techniques: Expressing

Journal: International Journal of Molecular and Cellular Medicine

Article Title: Sesamin Acts as Anti-leukemic Compound Interacting with Novel Phosphoprotein Targets and Inducing Apoptosis in Leukemic Cells

doi: 10.22088/IJMCM.BUMS.11.1.1

Figure Lengend Snippet: The schematic diagram shows the interactions between sesamin, caspase-3 (CASP3), caspase-7 (CASP7), caspase-8 (CASP8), caspase-9 (CASP9), BCL-2, and selected phosphoprotein; PARP4 and IPPK, in MOLT-4 and NB4 leukemic cell lines using STITCH database. The round shape represents protein. The oval shape represents chemical. Red circle indicates selective proteins. Red dashed circle indicates selective chemicals

Article Snippet: Leukemic Cell culture Acute lymphoblastic leukemia (ALL) MOLT-4 and acute promyelocytic leukemia (AML) NB4 cells purchased from Cell Lines Service GmbH (Eppelheim, Germany) were cultured in 10% fetal bovine serum and 2% penicillin-streptomycin supplements in RPMI-1640 medium at the condition of 37 o C and 5% CO 2 .

Techniques: