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Image Search Results
Journal: Scientific Reports
Article Title: Construction and validation of a cell based reporter assay for identifying inhibitors of SARS coronavirus 2 RNA dependent RNA polymerase activity
doi: 10.1038/s41598-025-03813-y
Figure Lengend Snippet: Validation of reporter assay using inhibitors of SARS-CoV-2 RdRp activity. HEK 293 cells and A549 cells were transiently transfected with the reporter vector and RdRp. After 24 h, the cells were treated with various concentrations of ( a ) remdesivir, ( b ) molnupiravir, ( c ) tenofovir, and ( d ) sofosbuvir. These compounds were added to the reporter vector and RdRp co-expressed cells, followed by an additional 24 h incubation to measure reporter activity (solid line) and cell viability (dotted line). The data represent the results of three independent experiments. Reporter activity was expressed as a percentage relative to the reporter and RdRp co-expressed group. Statistical significance is denoted as * p < 0.05, ** p < 0.01, and *** p < 0.001 compared to the reporter vector and RdRp co-expressed group.
Article Snippet:
Techniques: Biomarker Discovery, Reporter Assay, Activity Assay, Transfection, Plasmid Preparation, Incubation