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Regulation of LPS-induced cytokine secretion by LfP analogs. U937-derived macrophages were treated with <t>FKECHLA,</t> <t>FKECH,</t> <t>EKF</t> or YKE peptides (1–1000 µM) for 24 h and then treated with LPS (100 ng/mL, 24 h). Culture media was collected and its cytokine content was determined by standard ELISA for TNF-α ( A ), IL-6 ( B ), IL-10 ( C ) or TGF-β ( D ). Results are representative from three experiments and presented as mean ± SD with biological and analytical duplicates for each treatment. * indicate p < 0.05 compared to treatment with LPS alone. All treatments were statistically different from untreated cells, except cells treated by LPS and 1 or 10 μM FKECH in ( A ) (ANOVA).
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Regulation of LPS-induced cytokine secretion by LfP analogs. U937-derived macrophages were treated with <t>FKECHLA,</t> <t>FKECH,</t> <t>EKF</t> or YKE peptides (1–1000 µM) for 24 h and then treated with LPS (100 ng/mL, 24 h). Culture media was collected and its cytokine content was determined by standard ELISA for TNF-α ( A ), IL-6 ( B ), IL-10 ( C ) or TGF-β ( D ). Results are representative from three experiments and presented as mean ± SD with biological and analytical duplicates for each treatment. * indicate p < 0.05 compared to treatment with LPS alone. All treatments were statistically different from untreated cells, except cells treated by LPS and 1 or 10 μM FKECH in ( A ) (ANOVA).
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Regulation of LPS-induced cytokine secretion by LfP analogs. U937-derived macrophages were treated with <t>FKECHLA,</t> <t>FKECH,</t> <t>EKF</t> or YKE peptides (1–1000 µM) for 24 h and then treated with LPS (100 ng/mL, 24 h). Culture media was collected and its cytokine content was determined by standard ELISA for TNF-α ( A ), IL-6 ( B ), IL-10 ( C ) or TGF-β ( D ). Results are representative from three experiments and presented as mean ± SD with biological and analytical duplicates for each treatment. * indicate p < 0.05 compared to treatment with LPS alone. All treatments were statistically different from untreated cells, except cells treated by LPS and 1 or 10 μM FKECH in ( A ) (ANOVA).
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Regulation of LPS-induced cytokine secretion by LfP analogs. U937-derived macrophages were treated with <t>FKECHLA,</t> <t>FKECH,</t> <t>EKF</t> or YKE peptides (1–1000 µM) for 24 h and then treated with LPS (100 ng/mL, 24 h). Culture media was collected and its cytokine content was determined by standard ELISA for TNF-α ( A ), IL-6 ( B ), IL-10 ( C ) or TGF-β ( D ). Results are representative from three experiments and presented as mean ± SD with biological and analytical duplicates for each treatment. * indicate p < 0.05 compared to treatment with LPS alone. All treatments were statistically different from untreated cells, except cells treated by LPS and 1 or 10 μM FKECH in ( A ) (ANOVA).
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Regulation of LPS-induced cytokine secretion by LfP analogs. U937-derived macrophages were treated with FKECHLA, FKECH, EKF or YKE peptides (1–1000 µM) for 24 h and then treated with LPS (100 ng/mL, 24 h). Culture media was collected and its cytokine content was determined by standard ELISA for TNF-α ( A ), IL-6 ( B ), IL-10 ( C ) or TGF-β ( D ). Results are representative from three experiments and presented as mean ± SD with biological and analytical duplicates for each treatment. * indicate p < 0.05 compared to treatment with LPS alone. All treatments were statistically different from untreated cells, except cells treated by LPS and 1 or 10 μM FKECH in ( A ) (ANOVA).

Journal: International Journal of Molecular Sciences

Article Title: Resolution-Associated Lactoferrin Peptides Limit LPS Signaling and Cytokine Secretion from Human Macrophages

doi: 10.3390/ijms21145166

Figure Lengend Snippet: Regulation of LPS-induced cytokine secretion by LfP analogs. U937-derived macrophages were treated with FKECHLA, FKECH, EKF or YKE peptides (1–1000 µM) for 24 h and then treated with LPS (100 ng/mL, 24 h). Culture media was collected and its cytokine content was determined by standard ELISA for TNF-α ( A ), IL-6 ( B ), IL-10 ( C ) or TGF-β ( D ). Results are representative from three experiments and presented as mean ± SD with biological and analytical duplicates for each treatment. * indicate p < 0.05 compared to treatment with LPS alone. All treatments were statistically different from untreated cells, except cells treated by LPS and 1 or 10 μM FKECH in ( A ) (ANOVA).

Article Snippet: FKD, FKE, FKECH, FKECHLA, YKE, and EKF peptides were synthesized by GL Biochem (Shanghai) Ltd. (Shanghai, China) with purity typically over 90%.

Techniques: Derivative Assay, Enzyme-linked Immunosorbent Assay