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Image Search Results
Journal: Antioxidants
Article Title: TNFα Induces DNA and Histone Hypomethylation and Pulmonary Artery Smooth Muscle Cell Proliferation Partly via Excessive Superoxide Formation
doi: 10.3390/antiox13060677
Figure Lengend Snippet: TNF-α treatment decreases DNA methylation in PASMCs . DNA was purified from PASMCs treated with or without recombinant TNF-α (rTNF-α) for 24 or 48 h and the global DNA methylation was measured using an ELISA-based microplate assay that bound methylated DNA. ( A ) DNA methylation after 24 h of treatment. ( B ) DNA methylation after 48 h of treatment. ( C ) DNMT1 activity. ( D ) Cells were then pre-treated with tempol (1 mM) for 24 h prior to rTNF-α treatment for 24 h. DNA methylation was measured using the same method as described above. n = 3 independent replicates. ** p < 0.01 and *** p < 0.001 vs. untreated. ^ p < 0.05 vs. 200 ng TNF. ∆ p < 0.05 < 0.01 vs. ( A ); + p < 0.05, ++ p < 0.01 vs. ( D ); # p < 0.05 vs. ( B , C ).
Article Snippet: DNA was isolated from PASMCs treated with TNF-α (for 24 and 48 h) using a
Techniques: DNA Methylation Assay, Purification, Recombinant, Enzyme-linked Immunosorbent Assay, Methylation, Activity Assay
Journal: Antioxidants
Article Title: TNFα Induces DNA and Histone Hypomethylation and Pulmonary Artery Smooth Muscle Cell Proliferation Partly via Excessive Superoxide Formation
doi: 10.3390/antiox13060677
Figure Lengend Snippet: GADD45-α siRNA does not prevent the TNF-α-induced decrease in DNA methylation. ( A ) Real-time reverse transcription PCR was used to determine GADD45-α mRNA in PASMCs treated with rTNF-α for 24 or 48 h. PASMCs were treated with GADD45-α siRNA, negative siRNA, or lipofectamine only for 24 h prior to rTNF-α treatment for 24 h. ∆ p < 0.05, ∆∆ p < 0.01 vs. untreated; ( B ) Cell proliferation. ( C ) DNA methylation. n = 3 independent replicates. * p < 0.05 and ** p < 0.01 vs. GADD45 RNAi + 0 ng.
Article Snippet: DNA was isolated from PASMCs treated with TNF-α (for 24 and 48 h) using a
Techniques: DNA Methylation Assay, Reverse Transcription