Journal: Human Genomics and Proteomics : HGP

Article Title: Development of Potential Pharmacodynamic and Diagnostic Markers for Anti-IFN- α Monoclonal Antibody Trials in Systemic Lupus Erythematosus

doi: 10.4061/2009/374312

Figure Lengend Snippet: Relative expression of mRNAs and median fold changes (horizontal bars) of (a) type I IFN- α subtypes, (b) other members of the type I IFNs and IFN- α receptors, and (c) TNF- α , IFN- γ , and IFN- γ receptors in WB of SLE patients compared with healthy controls ( P ≤ .05 for all). Averages of relative mRNA levels of these cytokines and their receptors in WB from 24 healthy donors were scaled to 1 based on TaqMan QRT-PCR assays. IFN = interferon; QRT-PCR = quantitative real-time reverse transcriptase polymerase chain reaction; SLE = systemic lupus erythematosus; TNF- α = tumor necrosis factor.

Article Snippet: To measure the effects of SLE serum on the healthy donor cells, PBMCs were cultured at a density of 5 × 10 6 cells/mL in 250 μ L/well of a 24-well plate containing 25% SLE patient serum, in the presence or absence of the following neutralizing antibodies: anti-human-IFN- α (0.1, 1, and 10 μ g/mL; human IgG1; MedImmune, LLC.), anti-human IFN- γ (10 μ g/mL; mouse IgG1, clone MMHG-1; PBL), anti-human-IFNAR1 (10 μ g/mL; human IgG1; MedImmune, LLC.), and anti-HIVgp120 as a negative control (10 μ g/mL; human IgG1, MedImmune, LLC.).

Techniques: Expressing, Quantitative RT-PCR, Reverse Transcription, Polymerase Chain Reaction

Journal: Human Genomics and Proteomics : HGP

Article Title: Development of Potential Pharmacodynamic and Diagnostic Markers for Anti-IFN- α Monoclonal Antibody Trials in Systemic Lupus Erythematosus

doi: 10.4061/2009/374312

Figure Lengend Snippet: Representative heat map demonstrating anti-IFN- α , -IFNAR, and -IFN- γ mAb effects on healthy donor PBMC stimulated with serum from 1 SLE patient. Lane 1: SLE patient serum only; Lane 2: SLE patient serum plus reference antibody; Lane 3: SLE patient serum plus 10 μ g/mL anti-IFN- γ mAb; Lanes 4–6: SLE patient serum plus increasing concentrations of anti-IFN- α mAb (0.1, 1, and 10 μ g/mL); Lane 7: SLE patient serum plus 10 μ g/mL anti-IFNAR mAb. Color represents relative neutralization (inhibition) of overexpression of individual genes upregulated by soluble mediators in the serum of an SLE patient. The red color represents no neutralization, and green represents neutralization of overexpression of individual genes. IFN = interferon; IFNAR = interferon associated receptor; PBMC = peripheral blood mononuclear cells; SLE = systemic lupus erythematosus.

Article Snippet: To measure the effects of SLE serum on the healthy donor cells, PBMCs were cultured at a density of 5 × 10 6 cells/mL in 250 μ L/well of a 24-well plate containing 25% SLE patient serum, in the presence or absence of the following neutralizing antibodies: anti-human-IFN- α (0.1, 1, and 10 μ g/mL; human IgG1; MedImmune, LLC.), anti-human IFN- γ (10 μ g/mL; mouse IgG1, clone MMHG-1; PBL), anti-human-IFNAR1 (10 μ g/mL; human IgG1; MedImmune, LLC.), and anti-HIVgp120 as a negative control (10 μ g/mL; human IgG1, MedImmune, LLC.).

Techniques: Neutralization, Inhibition, Over Expression

Journal: Journal of molecular and cellular cardiology

Article Title: A protocol to study ex vivo mouse working heart at human-like heart rate

doi: 10.1016/j.yjmcc.2017.11.011

Figure Lengend Snippet: Increasing of Ca2+ in perfusion medium from 2.25 mM to 2.75 mM significantly increased both systolic and diastolic functions as shown by the higher LVP max, lower LVPmin (A), higher ±dP/dt (B) and stroke volume (C) of ex vivo mouse working hearts at heart rate of ~260 bpm, compensating the decrease from lowering heart rate from 480 bpm at 2.25 mM Ca2+. The test was done at 10 mmHg preload and 55 mmHg afterload. N = 6 hearts. Values are presented as mean ± SE. *P<0.05 vs. 2.25 mM Ca2+ control in Student’s t test.

Article Snippet: Within 2 min after the opening of the chest, Langendorff retrograde perfusion of the heart using Kreb’s solution at 37°C was established from the aortic cannula connected to an 80 mmHg perfusion system (Radnoti, US).

Techniques: Ex Vivo, Control

Journal: Frontiers in Physiology

Article Title: AltitudeOmics: Spontaneous Baroreflex Sensitivity During Acclimatization to 5,260 m: A Comparison of Methods

doi: 10.3389/fphys.2019.01505

Figure Lengend Snippet: Baroreflex sensitivity (BRS) estimation for the resting protocol at sea level. cl-40, clamp 40 mmHg of inspired CO 2 ; HVE, hyperventilation; REB, rebreathing up to PACO 2 of 50 mmHg. +Denotes outliers. ∗ Different from Seq ( p < 0.05).

Article Snippet: Following 10–15 min of quiet rest in a seated position, each experimental testing session consisted of (1) instrumentation; (2) 10 min in room air for baseline; (3) 10 min with end-tidal partial CO 2 pressure (PETCO 2 ) clamped at 40 mmHg (cl-40); (4) 3 min of voluntary hyperventilation to lower PETCO 2 to ∼20 mmHg (HVE); and (5) a modified rebreathing test (REB, details below).

Techniques:

Journal: Frontiers in Physiology

Article Title: AltitudeOmics: Spontaneous Baroreflex Sensitivity During Acclimatization to 5,260 m: A Comparison of Methods

doi: 10.3389/fphys.2019.01505

Figure Lengend Snippet: Baroreflex sensitivity estimation for the resting protocol on the 16 th day at 5,260 m. cl-40, clamp 40 mmHg of inspired CO 2 ; HVE, hyperventilation; REB, rebreathing up to PACO 2 of 50 mmHg. +Denotes outliers. ∗ Different from Seq ( p < 0.05).

Article Snippet: Following 10–15 min of quiet rest in a seated position, each experimental testing session consisted of (1) instrumentation; (2) 10 min in room air for baseline; (3) 10 min with end-tidal partial CO 2 pressure (PETCO 2 ) clamped at 40 mmHg (cl-40); (4) 3 min of voluntary hyperventilation to lower PETCO 2 to ∼20 mmHg (HVE); and (5) a modified rebreathing test (REB, details below).

Techniques:

Journal: Frontiers in Physiology

Article Title: AltitudeOmics: Spontaneous Baroreflex Sensitivity During Acclimatization to 5,260 m: A Comparison of Methods

doi: 10.3389/fphys.2019.01505

Figure Lengend Snippet: Baroreflex sensitivity estimation for the resting protocol on the first day at 5,260 m. cl-40, clamp 40 mmHg of inspired CO 2 ; HVE, hyperventilation; REB, rebreathing up to PACO 2 of 50 mmHg. +Denotes outliers. ∗ Different from Seq ( p < 0.05).

Article Snippet: Following 10–15 min of quiet rest in a seated position, each experimental testing session consisted of (1) instrumentation; (2) 10 min in room air for baseline; (3) 10 min with end-tidal partial CO 2 pressure (PETCO 2 ) clamped at 40 mmHg (cl-40); (4) 3 min of voluntary hyperventilation to lower PETCO 2 to ∼20 mmHg (HVE); and (5) a modified rebreathing test (REB, details below).

Techniques:

Journal: Journal of Cerebral Blood Flow & Metabolism

Article Title: Reactivity of larger intracranial arteries using 7 T MRI in young adults

doi: 10.1177/0271678X18762880

Figure Lengend Snippet: Hemodynamic parameters and cross-sectional area changes during the three breathing conditions.

Article Snippet: Between the two hemispheres, left and right arteries (eight vessels, four pairs) exhibited similar CVR in both the hypercapnic and the hypocapnic conditions. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Figure 2. caption a7 Cerebrovascular cross-sectional area reactivity (%CSA/mmHg of PetCO 2 ) to hypocapnia and hypercapnia across nine arteries.

Techniques:

Journal: Journal of Cerebral Blood Flow & Metabolism

Article Title: Reactivity of larger intracranial arteries using 7 T MRI in young adults

doi: 10.1177/0271678X18762880

Figure Lengend Snippet: Cerebrovascular cross-sectional area reactivity (%CSA/mmHg of PetCO2) to hypocapnia and hypercapnia across nine arteries. LPCA and RPCA: left and right posterior cerebral artery; LMCA and RMCA: left and right middle cerebral artery; LICA and RICA: left and right internal carotid artery; LACA and RACA: left and right anterior cerebral artery (n = 8 participants). Data presented as mean ± S.E.M. There was a difference between hypercapnia CVR and hypocapnia CVR at each vessel; paired t-test (P < 0.05).

Article Snippet: Between the two hemispheres, left and right arteries (eight vessels, four pairs) exhibited similar CVR in both the hypercapnic and the hypocapnic conditions. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Figure 2. caption a7 Cerebrovascular cross-sectional area reactivity (%CSA/mmHg of PetCO 2 ) to hypocapnia and hypercapnia across nine arteries.

Techniques: