Journal: Cellular microbiology

Article Title: NAD hydrolysis by the tuberculosis necrotizing toxin induces lethal oxidative stress in macrophages

doi: 10.1111/cmi.13115

Figure Lengend Snippet: Strains and plasmids used in this work.

Article Snippet: Data are represented as mean ± SEM. table ft1 table-wrap mode="anchored" t5 Table 1: caption a7 Strain/Plasmid Description Source or reference Strain M. tuberculosis H37Rv Wild-type ATCC 25618 M. tuberculosis ML845 H37Rv derivative, unmarked mutant of cpnT ( Danilchanka et al., 2014 ) M. tuberculosis ML2001 ML845 derivative, L5::pML3009, Kan R ( Danilchanka et al., 2014 ) M. tuberculosis ML2502 ML845 derivative, L5::pML3167, Kan R ( Pajuelo et al., 2018 ) Plasmid pML1995 pML1970 derivative, pBR322 origin, bla, lacIq, p T7lac -HIS- malE -POLYN-TEV- tnt-rv3902c , 7677 bp ( Sun et al., 2015 ) pML3009 PBR322 origin, aph, L5 attP, FRT-int-P imyc - esxF-esxE-cpnT-rv3902c -FRT, 9790 bp ( Danilchanka et al., 2014 ) pML3167 PBR322 origin, aph, L5 attP, FRT-int-P imyc - esxF-esxE-cpnT H792N/Q822K -rv3902c -FRT, 9789 bp ( Sun et al., 2015 ) Open in a separate window Strains and plasmids used in this work.

Techniques: Mutagenesis, Plasmid Preparation

Journal: Cellular microbiology

Article Title: NAD hydrolysis by the tuberculosis necrotizing toxin induces lethal oxidative stress in macrophages

doi: 10.1111/cmi.13115

Figure Lengend Snippet: THP-1 macrophages were infected with Mtb strains at an MOI of 10:1 and treated with N-acetylcysteine (NAC, 100 μM) when indicated. ROS levels were measured with the fluorescent probe H2DCFDA at (A) 4, 24 and 48 h or (B) 48 h post-infection. (C) Cell viability of infected macrophages was measured 48 h after infection as the total ATP content with a luminescent ATP detection assay kit. (D) The Mtb intracellular growth in infected macrophages was measured at 4 h and 48 h post-infection and expressed as CFU per ml. (E) Wt Mtb H37Rv was grown in Middlebrook 7H9 medium with 0.5% glycerol, 0.02% Tyloxapol and 10% OADC and supplemented with N-acetyl-cysteine (NAC, 100 μM). The OD600 was measured at the indicated time points. Asterisks indicate significant differences (p-value<0.01, calculated using the Two-way ANOVA with Bonferroni’s correction) compared with the indicated conditions. Data are represented as mean ± SEM.

Article Snippet: Data are represented as mean ± SEM. table ft1 table-wrap mode="anchored" t5 Table 1: caption a7 Strain/Plasmid Description Source or reference Strain M. tuberculosis H37Rv Wild-type ATCC 25618 M. tuberculosis ML845 H37Rv derivative, unmarked mutant of cpnT ( Danilchanka et al., 2014 ) M. tuberculosis ML2001 ML845 derivative, L5::pML3009, Kan R ( Danilchanka et al., 2014 ) M. tuberculosis ML2502 ML845 derivative, L5::pML3167, Kan R ( Pajuelo et al., 2018 ) Plasmid pML1995 pML1970 derivative, pBR322 origin, bla, lacIq, p T7lac -HIS- malE -POLYN-TEV- tnt-rv3902c , 7677 bp ( Sun et al., 2015 ) pML3009 PBR322 origin, aph, L5 attP, FRT-int-P imyc - esxF-esxE-cpnT-rv3902c -FRT, 9790 bp ( Danilchanka et al., 2014 ) pML3167 PBR322 origin, aph, L5 attP, FRT-int-P imyc - esxF-esxE-cpnT H792N/Q822K -rv3902c -FRT, 9789 bp ( Sun et al., 2015 ) Open in a separate window Strains and plasmids used in this work.

Techniques: Infection, Detection Assay

Journal: STAR Protocols

Article Title: Protocol for assessing myogenic tone and perfusion pressure in isolated mouse kidneys

doi: 10.1016/j.xpro.2024.102845

Figure Lengend Snippet:

Article Snippet: PowerLab , ADInstruments , ML845 4/25, or PL2604 4/26 series, or PowerLab C or similar alternative data acquisition hardware can be used.

Techniques: Recombinant